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Item 4293F READER - MD Spectra Max Gemini XS

4293F READER

 Molecular Devices SpectraMax Gemini XS

The Molecular Devices SpectraMax Gemini XS spectrofluorometer can detect signals either from fluorescence or luminescence based assays. The SpectraMax Gemini XS has all the features of a standard microplate reader (temperature control, shaker, etc.) as well as two monochromators to select desired wavelengths – this means that you won’t have to purchase or swap out filters, and that you can set the reader in 1nm increments. The SpectraMax Gemini XS can be used for both fluorescence and luminescence based assays. Along with the multi-well plate tray, this feature makes the Gemini XS one of the most versatile spectrophotometers on the market today. Applications include cellular and molecular binding studies, DNA quantitation, drug screening, and SNP genotyping analysis. Features include:

Dual Monochromators — The right pair of excitation and emission wavelengths is always available because the dual monochromators allow the selection of any wavelength in 1 nm increments. New fluorophores can easily be evaluated without purchasing additional filters.

 

 

Wavelength Scanning — The most sensitive results are achieved by using optimal excitation and emission wavelengths. Literature wavelengths are often based on results from wavelength-limited, filter-based readers. Wavelength scanning ensures that the most sensitive assay conditions are used.

 

 

 

Well Scanning — Gemini XS can report a single point from the well center, or multiple data points from large well tissue culture plates. This provides high sensitivity for cell-based assays.

 

 

 

Auto PMT Gain — Because a single microplate often presents a range of fluorescence intensities greater than three orders of magnitude, Gemini XS features “Auto PMT Gain” to avoid saturating the photomultiplier tube. The signal is calibrated against an internal standard, so the reported RFU values of individual samples can be accurately compared.

 

All of the equipment we sell is used and reconditioned. Our technicians test all equipment to confirm that it operates properly and to the manufacturer’s specifications. While we can’t test for a specific analytical application, we will do our best to simulate the conditions under which the equipment will be run. If you have particular testing requirements or requests please let us know and we’ll work with you to make sure these requirements are addressed during testing.

Specifications

 

 

 

Fluorescence photometric performance

Dual Monochromators

1 nm increment selection

 

EX Wavelength

250-850 nm

EM Wavelength

360-850 nm

 

Bandwidth (Excitation & Emission)

9 nm

 

Detection Limit (Signal 3X SD of Baseline)

2.0 fmol/well FITC 200 µL in 96 wells

 

Time Resolved Fluorescence (secondary mode)

 

Data Collection

50-1450 µsec, 200 µsec increments

 

Sensitivity

0.5 fmol/well

 

Luminescence Photometric Performance

 

Wavelength Range                                  250-850nm

 

Detection Limit

10 amol/well Alkaline Phosphatase 200 µl/well

 

General photometric specifications

Microplate Formats

6-, 12-, 24-, 48-, 96-, and 384-well

 

Light Source

Xenon flash lamp (1 joule/flash)

Detector

Photomultiplier (R-3896)

 

Read Time***

96 wells in <27 seconds
384 wells in <45 seconds

Shaker Time

0 to 999 seconds

 

Temperature Control (Chamber)

Ambient + 4 °C, to 45 °C

 

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4293F READER  -   MD Spectra Max Gemini XS

American Instrument Exchange, Inc.
1023 Western Avenue
Haverhill, MA 01832

Phone: 978-521-2221
Fax: 978-521-8822

EMAIL: info@americaninstrument.com