Nuaire S427-400 Class II/B1 Biosafety Cabinet

Nuaire NU427-600 Class II/B1 Biosafety Cabinet

by Nuaire

SKU 0878CC BIO CAB

FULLY TESTED & WARRANTIED*

Nuaire LabGard NU-427-600 Class II/Type B1 biological safety cabinet. 6’ wide model. 70%/30% exhausted air vs. recirculated air.

Quantity

Shipping

All products ship from our 30,000 sq. ft. Haverhill, MA warehouse

Contact Us

Call 978-521-2221
to request information & pricing for item 0878CC BIO CAB

Nuaire NU427-600 6’ Biological Safety Cabinet

The Nuaire NU427-600 6’ Biological Safety Cabinet is a Class II, Type B1 hood. This designation of biological safety cabinets is designed to protect the researcher from potentially dangerous samples, and the samples from contamination.

Nuaire NU427-600 - Specifications

Class II, Type B1
70% exhausted air vs. 30% recirculated air
Requires ducting
6’ wide (78” External / 70.5” Internal)
Includes new HEPA filters
90-day warranty

CLICK HERE to see the Nuaire NU427-600Class II/Type B1 biological safety cabinet on our YouTube channel.

NU427-600 Features

This B1 biosafety cabinet is carefully designed to handle work involving small amounts of volatile toxic chemicals or trace radionuclides. It ensures safety by directly exhausting these substances when used in the appropriate portion of the cabinet.

When recirculated in the downflow air, the system minimizes interference, allowing seamless microbiological studies. This dual functionality makes it versatile and reliable for complex laboratory applications.

These ventilated workstations offer positive pressure and three-sided enclosure, ensuring maximum containment and sample protection. They are ideal for microbiology, toxicology, and research labs, providing a safe and controlled workspace. The system draws air inward at a minimum inflow velocity of 100 ft/min, passing it through a HEPA filter. Finally, all air is safely exhausted outside through another HEPA filter and connected HVAC ductwork, preventing environmental contamination.

Testing and Certification

This Nuaire NU427-600 is used and reconditioned. Our technicians test each biosafety cabinet and clean bench to ensure that the controls and blower motor work properly, as well as ensuring that the HEPA filter is suitable for use in lab conditions. Our testing procedure includes all the elements of certification in order to ensure that the unit will pass certification once it is at your site.

If you're in the Greater Boston or New England area, contact our sister company, AIE Lab Services, to schedule your certification. In many cases, certification can be completed on the day of or following delivery. Their services include testing, certification, repair, replacement, decontamination, and removal of biosafety cabinets and clean benches. You can reach them at 978-521-2221 or by email at services@aielaboratoryservices.com.

Nuaire NU427-600 - Additional Specifications

External Dimensions	78”W x 33”D x 60”H
Interior work area dimensions	70.5”W x 23.5”D x 21”H
Electrical requirements	115V, 60Hz, 12 Amp

Pictures

The photos above represent our equipment, which is currently stored in our warehouse. Since we often stock multiple units of each model, we may not feature photos of every unit. However, if you request, we will provide photos of your specific equipment after we test and clean it.

For inquiries about the Nuaire NU427-600 6’ Biological Safety Cabinet, call us at 978-521-2221 or fill out the form on this page.

Testing Procedures for Used and Refurbished Biosafety Cabinets

Ensure Containment, Airflow Integrity, and Operator Safety

Used and refurbished biosafety cabinets (BSCs) from American Instrument Exchange are thoroughly tested to ensure they meet NSF/ANSI 49 performance standards for containment, airflow uniformity, and HEPA filtration. Each Class I, II, or III unit is evaluated through a series of specialized inspections and certifications, ensuring the cabinet performs reliably in research, clinical, and pharmaceutical lab environments.

Why Testing Used Biosafety Cabinets Is Essential

Before any biosafety cabinet is deployed in a working lab, it must be tested for:

• HEPA filter integrity and leak-free performance
• Inflow and downflow air velocity balance
• Containment efficiency and personnel protection
• Electrical system function and safety compliance
• Cleanliness and readiness for sterile applications

Proper testing of refurbished biosafety cabinets helps prevent cross-contamination, exposure to hazardous materials, and airflow disruption—critical for maintaining laboratory protocols. Our structured multi-point BSC testing protocol ensures dependable protection for operators, products, and the lab environment.

Key Testing Procedures for Refurbished and Used Biosafety Cabinets

1. Initial Visual Inspection

• Inspect exterior panels, window sashes, hinges, and seals for signs of wear or damage
• Confirm visibility and integrity of the viewing window
• Check power cords, display screens, and control interfaces for usability

2. Cabinet Decontamination and Cleaning

• Interior and exterior cleaned with non-corrosive disinfectants
• Decontamination protocols followed to remove biological contaminants
• Work surface, sidewalls, and return air grills are fully sanitized

3. HEPA Filter Integrity Testing (DOP/PAO Leak Test)

• Conduct aerosol challenge test using PAO or DOP to verify HEPA filter seal integrity
• Identify and resolve any filter leaks in accordance with NSF/ANSI 49 standards
• Ensure HEPA filters are correctly seated and uncompromised

4. Inflow and Downflow Velocity Testing

• Measure air velocities using calibrated thermo-anemometers
• Confirm inflow velocity meets requirements
• Verify downflow uniformity within the work zone to prevent turbulence and contamination

5. Airflow Smoke Pattern Testing

• Conduct visual smoke tests to demonstrate laminar airflow and containment
• Confirm that airflow barriers prevent cross-contamination and exposure

6. Electrical Safety and Alarm System Checks

• Inspect grounding, insulation resistance, and GFCI protection
• Test alarm functionality for sash position, airflow disruption, and power failure
• Verify interior lighting, outlets, and blower controls operate reliably

7. Final Certification and Quality Assurance

• Run full performance test cycle and confirm compliance with NSF/ANSI 49 and manufacturer specs
• Document data and generate a detailed service report

Guaranteed Performance from American Instrument Exchange

All refurbished biosafety cabinets from American Instrument Exchange are tested and verified to ensure maximum containment, user protection, and airflow performance. Whether used for microbiology, tissue culture, pharmaceutical compounding, or other work necessitating containment, our BSCs meet industry standards—so your team can work safely and confidently from day one.

Repair and Refurbishment Protocol for Used Biosafety Cabinets

Certified Protection and Performance from American Instrument Exchange

At American Instrument Exchange, every used biosafety cabinet (BSC) we offer is thoroughly repaired and refurbished to meet the highest standards for safety, airflow performance, and compliance. Whether it’s a Class I, Class II Type A2/B2, or Class III unit, we follow a meticulous refurbishment process to ensure your BSC provides reliable containment, sterile workspace conditions, and long-term durability.

Why Choose a Refurbished Biosafety Cabinet from American Instrument Exchange?

Fully tested and certified for airflow integrity and containment
Backed by warranty and performance guarantee
Cost-effective alternative to new biosafety cabinets
Environmentally responsible through expert reuse and restoration
Serviced by experienced technicians trained in NSF/ANSI 49 protocols

Step-by-Step Refurbishment Protocol

Every biosafety cabinet at American Instrument Exchange undergoes the following comprehensive multi-stage process to ensure it is lab-ready:

1. Initial Intake & Inspection

• Visual inspection of cabinet exterior, control panel, and sash mechanism
• Evaluation of hinges, seals, gaskets, and electrical components
• Missing parts or functional issues are documented for repair

2. Cleaning and Decontamination

• Full internal and external cleaning using non-abrasive, disinfectant-safe products
• Decontamination protocols followed to remove biohazardous or chemical residue
• Removable surfaces, work trays, and grilles are cleaned and sanitized

3. Mechanical and Electrical Repair

• Repair or replacement of worn components, including sash latches, lighting, and controls
• Electrical systems including switches, alarms, and outlets are tested and serviced
• Wiring is inspected and repaired to meet safety and performance standards

4. HEPA Filter Evaluation and Replacement

• Filters are tested for integrity using DOP/PAO aerosol challenge testing
• HEPA filters are replaced with OEM or equivalent filters
• Filter seals are verified for leak-free performance

5. Airflow and Performance Testing

• Inflow and downflow velocities are measured using calibrated instruments
• Smoke pattern testing confirms laminar airflow and containment effectiveness
• Blower motor function and airflow uniformity are verified across the cabinet

6. Safety System Verification

• Alarms for airflow disruption, sash height, and power loss are tested
• GFCI protection, lighting, and emergency shutoff systems are confirmed functional

7. Final Quality Control and Certification

• Performance testing is conducted to confirm NSF/ANSI 49 compliance (where applicable)
• Final visual inspection and cleaning performed prior to shipping
• Detailed service report and airflow test results provided with each unit

Trust the Experts at American Instrument Exchange

With over 50 years of experience in the lab equipment industry, American Instrument Exchange is a trusted name in refurbished biosafety cabinets. Our repair and refurbishment protocol ensures each cabinet provides dependable protection for personnel, products, and the environment—delivering maximum safety at a fraction of the cost of new.

Warranty Information

All used equipment sold by American Instrument Exchange is refurbished/reconditioned, tested to meet specifications, cleaned, and comes with a 90-day warranty from the date of shipment unless otherwise specified. New products include a warranty from the manufacturer. Your warranty is only valid if you have paid your invoice. If your invoice is over 30 days old, warranty work will not be performed until your invoice is paid in full. American Instrument Exchange is not liable for any special or consequential damages that result from the use of, or the inability to use, the materials on this site or the performance of the products,

Exceptions include international sales and items that are agreed to be sold with a lesser warranty or items that are agreed to be sold “as is.”

Should there be visible shipping damage to packaging or concealed shipping damage to item(s) supplied, buyer must communicate such damage to the delivering entity or carrier upon receipt first and to American Instrument the same day item is accepted / signed for. Failure to do so voids warranty. Customer decision to involve / procure third party or OEM service other than American Instrument or AIE assigned vendors to resolve in-warranty instrument failure or defect without first placing a service call to American Instrument voids warranty.

Return & Cancellation Policy

American Instrument Exchange will authorize the return of and issue a full refund for any equipment that is not working as represented and could not be replaced or repaired to the customer’s satisfaction by AIE or a representative of AIE during the warranty period. Returns without prior authorization and an RMA number will not be accepted.

Customers are responsible for proper packaging of the equipment and all included accessories. American Instrument will pay for insurance and shipping costs. Non-inventory items bought specifically to fill customer orders may possibly be returned, but on a case by case basis.

Customers who cancel orders after refurbishing has begun or after delivery where there are no equipment performance issues may be subject to a Restocking/Cancellation Fee of 20% or more and responsible for shipping costs for both the delivery and return.

Ongoing and Extended Support Options

American Instrument Exchange is able to provide additional repair and support services outside the warranty terms for most products that sell.

For biosafety cabinets, clean benches, chemical fume hoods, and other clean air products our team at AIE Laboratory Services can provide certifications, repairs, decontaminations, and other services for customers throughout New England, and guidance and limited technical assistance for any customers in the US and Canada for new or refurbished biosafety cabinets purchased from American Instrument Exchange.

For all other products, the repair service team at American Instrument Exchange can provide both field and depot repair evaluations. We can easily schedule and coordinate the pick-up and transportation of your malfunctioning equipment. By offering hourly rates lower than most OEM service providers and eliminating travel time for technicians, we help reduce repair costs. Furthermore, our lab depot repair service minimizes or eliminates scheduling delays. Our experienced service technicians are qualified to restore your equipment to factory specifications.

Please contact our team at info@americaninstrument.com for more details.

FAQ

Does the equipment come with a warranty?

Yes, all equipment from American Instrument Exchange comes with a warranty.
Used/reconditioned/refurbished equipment is tested to make sure it operates to the manufacturer’s specifications and comes with a 90-day warranty (unless otherwise noted). New equipment comes with a warranty from the manufacturer.

How do you test and recondition the equipment?

Testing requirements vary across all the equipment categories we sell. Temperature controlled units are tested with NIST traceable probes and data loggers. Gaskets, external housing, glass, handles, controls and control screens are all thoroughly inspected for integrity and functionality.
We test all equipment using the manufacturer's parameters and specifications to confirm that the unit operates properly. While we may not be able to test for specific analytical applications we do our best to simulate the conditions under which the equipment will be used in your lab. If you have particular testing requirements please let us know and we’ll work with you to make sure these are addressed during testing.

How do products ship from American Instrument Exchange?

We can ship products both nationally and internationally using a well-established network of transporters. Smaller items will ship via UPS. Larger equipment will be palletized and shipped either LTL or air-ride, depending on the specific equipment and/or delivery requirements. In many cases we can offer inside delivery. Customers are also welcome to make pick-up arrangements using their own preferred carrier.

All shipments are FOB Haverhill, MA. American Instrument Exchange will take reasonable steps to protect the equipment during shipment and the equipment will be fully insured. The customer will be charged for all shipping and handling charges, and fees incurred in the process of delivering the order.

For local customers in and around New England we can offer shipping and delivery using a local moving company trained by our staff to handle and deliver lab equipment. Our local movers can provide either standard dock-to-dock delivery or bring the equipment into your lab and set it in place.

Prior to signing the POD (Proof of Delivery), you are responsible for inspecting the goods for any damage. You MUST note any damage on the POD prior to letting the driver leave. This is to protect you, the receiver of the goods in the unlikely event of any damage. Once you sign the POD free and clear from damage, the goods are then deemed delivered in good order and no further claims would be applicable. This is the standard for all transportation so we want to protect you, our valued client.

Return and order cancellation policy

American Instrument Exchange will accept the return of any product that is not working as represented and could not be replaced or repaired to the customer’s satisfaction by American Instrument or a technician hired by/vetted by American Instrument Exchange’s during the warranty period. The customer will be responsible for proper packaging of the equipment and all included accessories.

Customers who cancel orders after refurbishing has begun or after delivery where there are no equipment performance issues will be subject to a cancellation and restocking fee of between 20% and 40% and responsible for the full total of the shipping & handling costs for both the delivery and the return of the equipment.

Are discounts available for educational or non-profit institutions, or for bulk purchases?

Yes – call or email us today with a list of equipment you’re looking for and we can potentially put together a quote that includes a discount.

How does your price matching policy work?

We strive to keep our pricing competitive and reasonable. If you find comparable items for sale at a lower price from other vendors please send us a link and or quote for the item and we’ll do our best to match or beat the price.

Can I see the equipment in person before committing to a purchase?

Customers are welcome to make an appointment to view equipment in our warehouse. Given our workload we may not be able to test or clean the equipment prior to your arrival if you have not committed to an order.

1023 Western Avenue is a working warehouse, not a showroom, and customers should dress appropriately and wear closed toe shoes.

Can I ship products that I purchased elsewhere to American Instrument for repair?

Yes – please see our repair services page for more information.