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Nuaire LabGard NU-425-400 Class II Type A2 Biosafety Cabinet – Refurbished
The Nuaire LabGard NU-425-400 biosafety cabinet is designed to provide personnel, product, and environmental protection for laboratory procedures requiring contamination-controlled airflow and sterile working conditions. Commonly used for cell culture, microbiology, pharmaceutical research, and biotechnology workflows, this refurbished Nuaire biosafety cabinet offers reliable containment performance for laboratories requiring advanced airflow protection and contamination-sensitive sample handling.
The NU-425-400 is part of Nuaire’s well-known LabGard platform and has been widely used throughout biotechnology laboratories, pharmaceutical facilities, hospital laboratories, academic research institutions, and cleanroom environments because of its durable welded-steel construction, stable airflow performance, ergonomic operating platform, and long-term reliability. The NU-425 series has historically been one of Nuaire’s most widely deployed Class II Type A2 biosafety cabinet platforms for general laboratory containment applications.
At American Instrument Exchange, each animal transfer station is inspected and tested by our technicians prior to shipment. Unless otherwise specified, new HEPA filters are installed at our facility before delivery. Laboratories and vivarium facilities outfitting containment-focused workflows may also browse additional refurbished biosafety cabinets, laboratory incubators, centrifuges, laboratory refrigerators, and freezers available through American Instrument Exchange.
Nuaire LabGard NU-425-400 Key Features
Nuaire LabGard NU-425-400 biosafety cabinet
Class II Type A2 biosafety cabinet
4-foot workspace configuration
Recirculating HEPA-filtered airflow system
30% exhaust / 70% recirculation airflow design
Suitable for microbiology and tissue culture workflows
Stainless steel work surface
HEPEX zero-leak airflow system
Ergonomic armrest configuration
Energy-efficient blower motor design
Includes stand and new HEPA filters
90-day warranty included from American Instrument Exchange
Refurbished and tested by American Instrument Exchange
Nuaire LabGard NU-425-400 Overview
The Nuaire LabGard NU-425-400 is a 4-foot Class II Type A2 biosafety cabinet engineered for laboratory environments requiring contamination control, sterile airflow, and operator protection. The cabinet operates using a recirculating HEPA-filtered airflow design in which approximately 70% of cabinet air is recirculated through the work area while approximately 30% is exhausted through HEPA filtration.
This airflow configuration may make the NU-425-400 especially appropriate for microbiology procedures, tissue culture workflows, biotechnology research, pharmaceutical preparation, sterile compounding procedures, and contamination-sensitive laboratory applications that do not require total exhaust operation.
The NU-425-400 operates using HEPA-filtered laminar airflow designed to maintain ISO Class 5 working conditions while supporting containment protection for biological workflows. Nuaire’s LabGard cabinet platforms are recognized throughout the laboratory industry for combining ergonomic operation, durable construction, stable airflow performance, and long-term reliability in demanding research environments.
Many NU-425 cabinets feature Nuaire’s HEPEX zero-leak airflow system, pressure-tight welded steel construction, ergonomic armrest design, stainless steel work surfaces, and removable work trays for easier cleaning and maintenance.
The compact 4-foot workspace configuration may make this biosafety cabinet especially appropriate for laboratories requiring contamination-controlled airflow while conserving valuable laboratory floor space. Many laboratories prefer 4-foot biosafety cabinets because they provide substantial sterile working area while fitting more easily into existing laboratory layouts.
The Nuaire LabGard NU-425-400 biosafety cabinet is commonly used for laboratory procedures requiring sterile airflow and contamination control. Typical applications may include tissue culture procedures, microbiology workflows, biotechnology research, pharmaceutical preparation, biological sample handling, and contamination-sensitive laboratory applications.
This biosafety cabinet may also be appropriate for biotechnology laboratories, pharmaceutical production environments, hospital laboratories, clinical testing laboratories, academic research facilities, and cleanroom applications requiring a Class II Type A2 biosafety cabinet.
This biosafety cabinet may be suitable for biotechnology laboratories, pharmaceutical production environments, hospital laboratories, academic research facilities, clinical diagnostics laboratories, GMP facilities, and cleanroom applications.
Its compact 4-foot footprint and ergonomic operating design may make it especially appropriate for laboratories requiring contamination-controlled airflow while maximizing available laboratory space.
Nuaire LabGard NU-425-400 Key Specifications
Specification
Details
Manufacturer
Nuaire
Model
LabGard NU-425-400
Cabinet Class
Class II Type A2
Nominal Width
4 ft
Airflow Design
Recirculating HEPA-filtered airflow
Airflow Ratio
30% exhaust / 70% recirculation
Inflow Velocity
Approx. 105 FPM
Downflow Velocity
Approx. 60 FPM
Filtration
HEPA-filtered supply and exhaust airflow
Work Surface
Stainless steel
Cabinet Construction
Pressure-tight welded steel construction
Plenum Design
HEPEX zero-leak airflow system
Exterior Dimensions
Approx. 53.6” W × 32.9” D × 63” H
Interior Dimensions
Approx. 46.5” W × 23.5” D × 28.5” H
Weight
Approx. 500 lbs
Sash Opening
8” or 10” operating height
Electrical Requirements
115V, 60Hz
Included Accessories
Stand and new HEPA filters
Warranty
90-day warranty
Condition
Refurbished
Specifications compiled from Nuaire manufacturer literature, distributor technical references, and American Instrument Exchange product information.
Nuaire LabGard NU-425-400 Refurbishment and Testing
American Instrument Exchange has extensive experience refurbishing used biosafety cabinets and laboratory containment equipment for research and pharmaceutical environments.
Each biosafety cabinet is inspected by our technicians to verify proper blower operation, airflow performance, sash operation, control operation, lighting operation, cabinet integrity, and overall system condition prior to shipment. Unless otherwise specified, new HEPA filters are installed at our facility before delivery.
Testing procedures include major functional and airflow verification elements associated with biosafety cabinet performance to help support certification readiness upon installation at your facility.
Nuaire LabGard NU-425-400 Certification Readiness
Class II Type A2 biosafety cabinets should be certified by qualified field technicians after installation and before laboratory use.
American Instrument Exchange performs functional testing prior to shipment to support certification readiness upon arrival. Buyers should confirm facility compatibility, airflow requirements, electrical requirements, and certification standards prior to installation.
Certification services are available throughout New England through AIE Laboratory Services, which provides biosafety cabinet certification, preventative maintenance, testing, repair, and field service support for laboratory containment equipment.
Certification requirements may vary depending on laboratory protocols, facility standards, and application requirements.
Nuaire LabGard NU-425-400 Shipping and Installation Considerations
Biosafety cabinets are oversized laboratory containment systems that may require special handling during transportation, delivery, and installation. Buyers should confirm facility access dimensions, doorway clearances, elevator compatibility, electrical compatibility, and final cabinet placement before shipment.
Depending on the installation environment, liftgate delivery, inside delivery services, rigging assistance, or coordinated facility access may be recommended.
The 4-foot cabinet configuration may provide easier transport and placement flexibility compared to larger biosafety cabinet platforms.
Why has the NU-425 series remained popular in laboratories for so many years?
The NU-425 series became widely adopted because it combines durable welded-steel construction, dependable airflow performance, ergonomic design, and relatively straightforward serviceability for long-term laboratory use.
Is the NU-425-400 appropriate for tissue culture applications?
Yes. The NU-425-400 is commonly used for tissue culture, microbiology, biotechnology research, and sterile pharmaceutical preparation workflows requiring Class II Type A2 containment.
What is the advantage of the HEPEX airflow system?
Nuaire’s HEPEX system is designed to create a pressure-tight airflow plenum intended to minimize the risk of contaminated air leakage while improving long-term airflow stability.
Why do many laboratories prefer 4-foot biosafety cabinets?
A 4-foot biosafety cabinet provides substantial sterile working area while fitting more easily into crowded laboratory environments compared to larger 5-foot or 6-foot cabinets.
Does American Instrument Exchange replace HEPA filters before shipment?
Yes. Unless otherwise specified, American Instrument Exchange installs new HEPA filters before shipment. Cabinets are inspected and tested by our technicians prior to delivery to help support certification readiness after installation.
What testing does American Instrument Exchange perform during refurbishment?
American Instrument Exchange technicians inspect airflow systems, blower operation, sash functionality, cabinet controls, lighting systems, and overall cabinet condition prior to shipment.
Can American Instrument Exchange help coordinate biosafety cabinet certification?
Yes. AIE Laboratory Services provides biosafety cabinet certification, preventative maintenance, repair, testing, and field service support throughout New England for many Nuaire containment systems.
Does the cabinet include a warranty?
Yes. A 90-day warranty from American Instrument Exchange is included.
Click here to see the Nuaire NU425-400 on the American Instrument Exchange YouTube channel. Video incorrectly states the model as the 5' NU425-500.
Testing Procedures for Used and Refurbished Biosafety Cabinets
Ensure Containment, Airflow Integrity, and Operator Safety
Used and refurbished biosafety cabinets (BSCs) from American Instrument Exchange are thoroughly tested to ensure they meet NSF/ANSI 49 performance standards for containment, airflow uniformity, and HEPA filtration. Each Class I, II, or III unit is evaluated through a series of specialized inspections and certifications, ensuring the cabinet performs reliably in research, clinical, and pharmaceutical lab environments.
Why Testing Used Biosafety Cabinets Is Essential
Before any biosafety cabinet is deployed in a working lab, it must be tested for:
• HEPA filter integrity and leak-free performance
• Inflow and downflow air velocity balance
• Containment efficiency and personnel protection
• Electrical system function and safety compliance
• Cleanliness and readiness for sterile applications
Proper testing of refurbished biosafety cabinets helps prevent cross-contamination, exposure to hazardous materials, and airflow disruption—critical for maintaining laboratory protocols. Our structured multi-point BSC testing protocol ensures dependable protection for operators, products, and the lab environment.
Key Testing Procedures for Refurbished and Used Biosafety Cabinets
1. Initial Visual Inspection
• Inspect exterior panels, window sashes, hinges, and seals for signs of wear or damage
• Confirm visibility and integrity of the viewing window
• Check power cords, display screens, and control interfaces for usability
2. Cabinet Decontamination and Cleaning
• Interior and exterior cleaned with non-corrosive disinfectants
• Decontamination protocols followed to remove biological contaminants
• Work surface, sidewalls, and return air grills are fully sanitized
• Conduct aerosol challenge test using PAO or DOP to verify HEPA filter seal integrity
• Identify and resolve any filter leaks in accordance with NSF/ANSI 49 standards
• Ensure HEPA filters are correctly seated and uncompromised
4. Inflow and Downflow Velocity Testing
• Measure air velocities using calibrated thermo-anemometers
• Confirm inflow velocity meets requirements
• Verify downflow uniformity within the work zone to prevent turbulence and contamination
5. Airflow Smoke Pattern Testing
• Conduct visual smoke tests to demonstrate laminar airflow and containment
• Confirm that airflow barriers prevent cross-contamination and exposure
6. Electrical Safety and Alarm System Checks
• Inspect grounding, insulation resistance, and GFCI protection
• Test alarm functionality for sash position, airflow disruption, and power failure
• Verify interior lighting, outlets, and blower controls operate reliably
7. Final Certification and Quality Assurance
• Run full performance test cycle and confirm compliance with NSF/ANSI 49 and manufacturer specs
• Document data and generate a detailed service report
Guaranteed Performance from American Instrument Exchange
All refurbished biosafety cabinets from American Instrument Exchange are tested and verified to ensure maximum containment, user protection, and airflow performance. Whether used for microbiology, tissue culture, pharmaceutical compounding, or other work necessitating containment, our BSCs meet industry standards—so your team can work safely and confidently from day one.
Repair and Refurbishment Protocol for Used Biosafety Cabinets
Certified Protection and Performance from American Instrument Exchange
At American Instrument Exchange, every used biosafety cabinet (BSC) we offer is thoroughly repaired and refurbished to meet the highest standards for safety, airflow performance, and compliance. Whether it’s a Class I, Class II Type A2/B2, or Class III unit, we follow a meticulous refurbishment process to ensure your BSC provides reliable containment, sterile workspace conditions, and long-term durability.
Why Choose a Refurbished Biosafety Cabinet from American Instrument Exchange?
Fully tested and certified for airflow integrity and containment
Backed by warranty and performance guarantee
Cost-effective alternative to new biosafety cabinets
Environmentally responsible through expert reuse and restoration
Serviced by experienced technicians trained in NSF/ANSI 49 protocols
Step-by-Step Refurbishment Protocol
Every biosafety cabinet at American Instrument Exchange undergoes the following comprehensive multi-stage process to ensure it is lab-ready:
1. Initial Intake & Inspection
• Visual inspection of cabinet exterior, control panel, and sash mechanism
• Evaluation of hinges, seals, gaskets, and electrical components
• Missing parts or functional issues are documented for repair
2. Cleaning and Decontamination
• Full internal and external cleaning using non-abrasive, disinfectant-safe products
• Decontamination protocols followed to remove biohazardous or chemical residue
• Removable surfaces, work trays, and grilles are cleaned and sanitized
3. Mechanical and Electrical Repair
• Repair or replacement of worn components, including sash latches, lighting, and controls
• Electrical systems including switches, alarms, and outlets are tested and serviced
• Wiring is inspected and repaired to meet safety and performance standards
4. HEPA Filter Evaluation and Replacement
• Filters are tested for integrity using DOP/PAO aerosol challenge testing
• HEPA filters are replaced with OEM or equivalent filters
• Filter seals are verified for leak-free performance
5. Airflow and Performance Testing
• Inflow and downflow velocities are measured using calibrated instruments
• Smoke pattern testing confirms laminar airflow and containment effectiveness
• Blower motor function and airflow uniformity are verified across the cabinet
6. Safety System Verification
• Alarms for airflow disruption, sash height, and power loss are tested
• GFCI protection, lighting, and emergency shutoff systems are confirmed functional
7. Final Quality Control and Certification
• Performance testing is conducted to confirm NSF/ANSI 49 compliance (where applicable)
• Final visual inspection and cleaning performed prior to shipping
• Detailed service report and airflow test results provided with each unit
Trust the Experts at American Instrument Exchange
With over 50 years of experience in the lab equipment industry, American Instrument Exchange is a trusted name in refurbished biosafety cabinets. Our repair and refurbishment protocol ensures each cabinet provides dependable protection for personnel, products, and the environment—delivering maximum safety at a fraction of the cost of new.
Warranty Information
All used equipment sold by American Instrument Exchange is refurbished/reconditioned, tested to meet specifications, cleaned, and comes with a 90-day warranty from the date of shipment unless otherwise specified. New products include a warranty from the manufacturer. Your warranty is only valid if you have paid your invoice. If your invoice is over 30 days old, warranty work will not be performed until your invoice is paid in full. American Instrument Exchange is not liable for any special or consequential damages that result from the use of, or the inability to use, the materials on this site or the performance of the products,
Exceptions include international sales and items that are agreed to be sold with a lesser warranty or items that are agreed to be sold “as is.”
Should there be visible shipping damage to packaging or concealed shipping damage to item(s) supplied, buyer must communicate such damage to the delivering entity or carrier upon receipt first and to American Instrument the same day item is accepted / signed for. Failure to do so voids warranty. Customer decision to involve / procure third party or OEM service other than American Instrument or AIE assigned vendors to resolve in-warranty instrument failure or defect without first placing a service call to American Instrument voids warranty.
Return & Cancellation Policy
American Instrument Exchange will authorize the return of and issue a full refund for any equipment that is not working as represented and could not be replaced or repaired to the customer’s satisfaction by AIE or a representative of AIE during the warranty period. Returns without prior authorization and an RMA number will not be accepted.
Customers are responsible for proper packaging of the equipment and all included accessories. American Instrument will pay for insurance and shipping costs. Non-inventory items bought specifically to fill customer orders may possibly be returned, but on a case by case basis.
Customers who cancel orders after refurbishing has begun or after delivery where there are no equipment performance issues may be subject to a Restocking/Cancellation Fee of 20% or more and responsible for shipping costs for both the delivery and return.
Ongoing and Extended Support Options
American Instrument Exchange is able to provide additional repair and support services outside the warranty terms for most products that sell.
For biosafety cabinets, clean benches, chemical fume hoods, and other clean air products our team at AIE Laboratory Services can provide certifications, repairs, decontaminations, and other services for customers throughout New England, and guidance and limited technical assistance for any customers in the US and Canada for new or refurbished biosafety cabinets purchased from American Instrument Exchange.
For all other products, the repair service team at American Instrument Exchange can provide both field and depot repair evaluations. We can easily schedule and coordinate the pick-up and transportation of your malfunctioning equipment. By offering hourly rates lower than most OEM service providers and eliminating travel time for technicians, we help reduce repair costs. Furthermore, our lab depot repair service minimizes or eliminates scheduling delays. Our experienced service technicians are qualified to restore your equipment to factory specifications.
Yes, all equipment from American Instrument Exchange comes with a warranty. Used/reconditioned/refurbished equipment is tested to make sure it operates to the manufacturer's specifications and comes with a 90-day warranty (unless otherwise noted). New equipment comes with a warranty from the manufacturer.
How do you test and recondition the equipment?
Testing requirements vary across all the equipment categories we sell. Temperature controlled units are tested with NIST traceable probes and data loggers. Gaskets, external housing, glass, handles, controls and control screens are all thoroughly inspected for integrity and functionality. We test all equipment using the manufacturer's parameters and specifications to confirm that the unit operates properly.
How do products ship from American Instrument Exchange?
We can ship products both nationally and internationally using a well-established network of transporters. Smaller items will ship via UPS. Larger equipment will be palletized and shipped either LTL or air-ride, depending on the specific equipment and/or delivery requirements.
Return and order cancellation policy
American Instrument Exchange will accept the return of any product that is not working as represented and could not be replaced or repaired to the customer's satisfaction during the warranty period. Customers who cancel orders after refurbishing has begun will be subject to a cancellation and restocking fee of between 20% and 40%.
Are discounts available for educational or non-profit institutions, or for bulk purchases?
Yes – call or email us today with a list of equipment you're looking for and we can potentially put together a quote that includes a discount.
How does your price matching policy work?
We strive to keep our pricing competitive and reasonable. If you find comparable items for sale at a lower price from other vendors please send us a link and or quote for the item and we'll do our best to match or beat the price.
Can I see the equipment in person before committing to a purchase?
Customers are welcome to make an appointment to view equipment in our warehouse. Given our workload we may not be able to test or clean the equipment prior to your arrival if you have not committed to an order.
1023 Western Avenue is a working warehouse, not a showroom, and customers should dress appropriately and wear closed toe shoes.
Can I ship products that I purchased elsewhere to American Instrument for repair?
Yes – please see our repair services page for more information.
