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Eppendorf 5425R Refrigerated Microcentrifuge with FA-24-2 Rotor
American Instrument Exchange offers used and refurbished laboratory centrifuges for sale, including refrigerated centrifuges, floor model centrifuges, benchtop centrifuges, and large-capacity bioprocessing centrifuges from leading manufacturers including Thermo Scientific, Beckman Coulter, Eppendorf, and more.
The Eppendorf 5425R is a refrigerated microcentrifuge designed for molecular biology laboratories, biotechnology applications, pharmaceutical workflows, clinical research environments, and general laboratory sample processing requiring high-speed microcentrifugation in a compact benchtop footprint. The 5425R combines refrigerated operation, high RCF performance, rapid cooling capability, and intuitive digital controls to support temperature-sensitive molecular biology and life science workflows.
Laboratories evaluating refrigerated microcentrifuges often compare maximum RPM and RCF performance, cooling capability, rotor flexibility, throughput capacity, and compact footprint design when selecting a centrifuge system. This particular Eppendorf 5425R configuration includes the Eppendorf FA-24-2 fixed-angle rotor, providing high-speed processing capability for up to 24 x 1.5/2.0 mL microcentrifuge tubes.
At American Instrument Exchange, our technicians inspect and test each centrifuge to verify proper refrigeration performance, motor operation, braking systems, lid lock functionality, and controller operation prior to shipment.
Features of the Eppendorf 5425R
Refrigerated microcentrifuge
Includes Eppendorf FA-24-2 fixed-angle rotor
Maximum speed of 15,060 RPM
Maximum force of 21,300 x g
24-place microtube capacity
Compact benchtop footprint
FastTemp rapid pre-cooling functionality
Temperature range from -10°C to +40°C
ECO shut-off refrigeration technology
Quiet laboratory operation
Automatic rotor recognition
Digital control interface
Short-spin quick centrifugation functionality
Supports molecular biology and microtube workflows
Compatible with PCR strips and spin columns depending on rotor configuration
90-day warranty included from American Instrument Exchange
Refurbished and tested by American Instrument Exchange
The included FA-24-2 aerosol-tight fixed-angle rotor is designed for high-speed molecular biology workflows and supports up to 24 x 1.5/2.0 mL tubes with maximum speeds up to 15,060 RPM.
Overview of the Eppendorf 5425R Refrigerated Microcentrifuge
The Eppendorf 5425R is a refrigerated benchtop microcentrifuge optimized for molecular biology, genomics, proteomics, PCR preparation, nucleic acid purification, and other life science laboratory applications requiring high-speed refrigerated sample processing. Unlike larger clinical centrifuges or floor model centrifuge systems designed for large-volume workflows, the 5425R is engineered specifically for microtube applications where compact size, refrigeration stability, and rapid acceleration are essential.
The included FA-24-2 fixed-angle rotor accommodates 24 x 1.5/2.0 mL tubes and is commonly used for DNA and RNA extraction, protein purification, spin column workflows, microbiology applications, and molecular biology sample preparation. The refrigerated chamber helps maintain stable temperatures during centrifugation, making the 5425R particularly well suited for temperature-sensitive DNA, RNA, protein, and enzyme workflows where sample integrity is critical.
The combination of compact footprint, high-speed operation, advanced refrigeration technology, and intuitive controls makes the Eppendorf 5425R especially valuable in shared research laboratories, biotechnology facilities, pharmaceutical laboratories, and academic molecular biology environments where bench space and throughput flexibility are important considerations.
Browse additional used centrifuges for sale from American Instrument Exchange.
Common Applications for the Eppendorf 5425R
The Eppendorf 5425R is commonly used for DNA and RNA extraction, PCR sample preparation, protein purification, molecular biology workflows, genomics research, proteomics applications, spin column processing, microbiology workflows, and general laboratory microcentrifugation procedures. The included FA-24-2 fixed-angle rotor allows laboratories to process up to 24 microcentrifuge tubes simultaneously, improving workflow efficiency in busy molecular biology and biotechnology environments. The refrigerated chamber helps maintain stable sample temperatures during centrifugation, making the 5425R particularly well suited for temperature-sensitive molecular biology and life science applications where preserving sample integrity is essential.
Rotor Compatibility and Included Accessories
Rotor compatibility is one of the most important considerations when purchasing a laboratory microcentrifuge. The Eppendorf 5425R is designed to support a wide range of molecular biology and microtube workflows depending on installed rotor configuration.
This particular system includes:
Eppendorf FA-24-2 fixed-angle rotor
Rotor lid
Power cord
Depending on configuration, the Eppendorf 5425R are able to support:
Standard microcentrifuge tubes
PCR strips
Spin column applications
Molecular biology workflows
Genomics applications
Proteomics sample preparation
The included FA-24-2 rotor supports up to 24 x 1.5/2.0 mL tubes and provides maximum performance up to 15,060 RPM and 21,300 x g.
Customers should verify included rotors prior to purchase, as centrifuge configurations can vary between systems.
Specifications for the Eppendorf 5425R
Specification
Details
Manufacturer
Eppendorf
Model
5425R
Centrifuge Type
Refrigerated Microcentrifuge
Included Rotor
FA-24-2 Fixed-Angle Rotor
Maximum Speed
15,060 RPM
Maximum RCF
21,300 x g
Rotor Capacity
24 x 1.5/2.0 mL
Temperature Range
-10°C to +40°C
Refrigeration Features
FastTemp and ECO Shut-Off
Electrical Requirements
120V, 50/60Hz
Dimensions
Approx. 11.4" W x 18.9" D x 10.2" H
Weight
Approx. 46 lbs
Specifications are provided to assist with planning and installation. Rotor compatibility, included accessories, and system configuration may vary depending on the specific unit available for sale.
Power Requirements and Shipping Considerations
The Eppendorf 5425R typically requires 120V, 50/60Hz electrical service and is designed for benchtop laboratory installation. Laboratories should verify electrical compatibility, available bench space, and ventilation requirements prior to purchasing this centrifuge.
Although the 5425R is compact compared to larger refrigerated centrifuges, the system still contains refrigeration components and sensitive internal electronics that typically require protective freight or carefully packaged shipment procedures during transport. Laboratories should verify installation space and electrical compatibility prior to delivery.
American Instrument Exchange can assist customers with shipping coordination, freight preparation, and installation planning for refrigerated laboratory centrifuges.
Refurbished Centrifuges from American Instrument Exchange
American Instrument Exchange specializes in refurbished laboratory centrifuges including refrigerated centrifuges, floor model centrifuges, benchtop centrifuges, blood banking centrifuges, and bioprocessing centrifuge systems.
Our technicians inspect and test centrifuges for:
Refrigeration performance
Rotor operation
Brake functionality
Controller operation
Lid lock safety systems
General operational reliability
If the centrifuge is sold with a rotor, we also test the rotor and centrifuge combination together to verify compatibility and operational performance.
American Instrument Exchange and AIE Laboratory Services provide centrifuge repair, preventive maintenance, diagnostics, and laboratory equipment service for many Eppendorf centrifuge models.
The Eppendorf 5424R is a refrigerated microcentrifuge designed for molecular biology and life science applications such as DNA/RNA extraction, PCR prep, and protein analysis. With support for standard 1.5/2.0 mL tubes and spin columns, it’s ideal for clinical labs, biotech research, and academic laboratories.
What are the key specifications of the Eppendorf 5424R?
The 5424R combines high performance with a compact footprint:
How reliable is a refurbished Eppendorf 5424R centrifuge?
Eppendorf centrifuges are known for durability and long service life. When refurbished, critical components such as bearings and seals, cooling systems, and electrical controls are tested and serviced to ensure dependable operation. Many labs rely on refurbished 5424R units for daily, high-throughput workflows.
Does the Eppendorf 5424R maintain accurate temperature control?
Yes. The 5424R features advanced refrigeration with:
Temperature range from -10°C to +40°C
Ability to reach 4°C quickly (≈8 minutes)
Stable temperature control even at maximum speed
This ensures optimal protection for temperature-sensitive samples like enzymes and nucleic acids.
Is the Eppendorf 5424R easy to use?
Yes, it is designed for intuitive operation with:
Simple rotary knobs or digital controls
One-finger lid closure
Short-spin function for quick runs
Clear digital display of speed and time
These features streamline workflows and reduce user error.
How energy efficient is the Eppendorf 5424R?
The centrifuge includes energy-saving features such as:
ECO shut-off mode after inactivity
Reduced power consumption during standby
Efficient cooling system
This can reduce energy usage by up to 40% overnight, lowering operating costs.
Why should I buy a used or refurbished Eppendorf 5424R?
Environmentally responsible reuse of high-quality equipment
Each unit is inspected for rotor balance, temperature accuracy, and mechanical integrity before shipment.
How does the 5424R compare to newer centrifuge models?
The 5424R remains a lab-standard microcentrifuge thanks to its:
High speed and RCF
Reliable refrigeration
Compact design
For many labs, it delivers the same core functionality as newer models at a fraction of the cost, making it a smart investment.
Why buy from American Instrument Exchange?
American Instrument Exchange provides:
Thoroughly tested and reconditioned equipment
Fast shipping from a U.S. warehouse
Expert support and responsive service
Competitive pricing on premium lab instruments
This makes them a trusted source for used Eppendorf centrifuges and other laboratory equipment.
CLICK HEREto see the Eppendorf 5425R on our YouTube channel
Testing Procedures for Used and Refurbished Laboratory Centrifuges
Ensure Quality, Accuracy, and Compliance Before Deployment
Used and refurbished laboratory centrifuges from American Instrument Exchange undergo testing procedures to ensure they meet the performance, safety, and compliance standards required in modern laboratories.
Why Testing Used Laboratory Centrifuges Is Essential
Before a centrifuge is reintroduced into a lab environment, it must be tested for:
Mechanical integrity
Rotor balance and speed accuracy
Safety system functionality
Electrical compliance
Proper testing helps extend equipment lifespan, reduce downtime, and ensure safety in high-stakes lab environments such as clinical diagnostics, biotechnology, and research applications.
Our structured testing protocol for used and refurbished centrifuges ensures equipment safety, accuracy, and longevity.
Key Testing Procedures for Refurbished and Used Centrifuges
1. Initial Visual Inspection
Check for signs of wear, corrosion, or damage to housing, lid, and control panels
Inspect power cords, plugs, and connectors for fraying or exposed wiring
Confirm that the rotor and accessories are compatible and undamaged
2. Functional Testing
Power up the centrifuge and confirm successful boot-up of digital or analog displays
Test lid lock and emergency stop mechanisms
Run initial cycles at various speeds to check for abnormal noise or vibration
3. Rotor and Speed Verification
Test all programmable speed settings to confirm consistent acceleration and deceleration
Monitor temperature, vibration, and RPM consistency throughout the cycle
Balance testing ensures smooth operation and prevents wear on internal components
4. Electrical Safety and Grounding Tests
Verify that all electrical components are properly grounded and shielded
5. Temperature Control (if applicable)
For refrigerated centrifuges, test cooling function to ensure temperature stability within set ranges
Check refrigerant levels and inspect for compressor noise or failure
6. Final Quality Assurance Checklist
Conduct a full run-through using the user interface
Document all test results
Repair and Refurbishment Protocol for Used Laboratory Centrifuges
Certified Quality from American Instrument Exchange
At American Instrument Exchange, we follow a meticulous repair and refurbishment protocol to ensure that every used laboratory centrifuge we sell. We strive for reliable performance and long-term durability—providing labs with exceptional value on high-quality pre-owned centrifuges.
Why Choose a Refurbished Centrifuge from American Instrument Exchange?
Fully tested
Backed by warranty and performance guarantee
Cost-effective alternative to new lab equipment
Environmentally responsible through equipment reuse
Supported by expert technicians with decades of experience
Step-by-Step Refurbishment Protocol
Every used centrifuge at American Instrument Exchange goes through the following comprehensive repair and refurbishment process:
1. Initial Intake & Inspection
Our technicians perform a complete visual and mechanical inspection
Evaluation of hinges, seals, gaskets, and electrical components
Any physical damage, missing parts, or wear is documented for repair
2. Cleaning and decontamination
Full internal and external cleaning using non-abrasive, disinfectant-safe products
Biological or chemical residue is decontaminated/cleaned
3. Mechanical & Electrical Repairs
Damaged or worn parts (e.g., drive motors, lid latches, rotor buckets) are replaced or repaired
Electrical components, sensors, and fuses are tested and replaced as needed
Wiring is inspected for integrity and safety per industry standards
4. Rotor & Accessory Evaluation
All rotors, buckets, and adapters are inspected for cracks, corrosion, and balance
Non-conforming components are replaced with OEM or equivalent alternatives
5. Performance Testing
Rotor speed (RPM), acceleration, and deceleration are verified
Refrigerated centrifuges undergo temperature stability testing across set ranges
6. Safety System Verification
Lid lock and interlock systems are tested for proper function
Emergency stop and imbalance detection systems are verified
Electrical safety and grounding tests are conducted
Trust the Experts at American Instrument Exchange
With over 50 years of experience in the industry, American Instrument Exchange is a trusted name for refurbished lab equipment. Our centrifuge refurbishment protocol reflects our commitment to quality, safety, and customer satisfaction.
Warranty Information
All used equipment sold by American Instrument Exchange is refurbished/reconditioned, tested to meet specifications, cleaned, and comes with a 90-day warranty from the date of shipment unless otherwise specified. New products include a warranty from the manufacturer. Your warranty is only valid if you have paid your invoice. If your invoice is over 30 days old, warranty work will not be performed until your invoice is paid in full. American Instrument Exchange is not liable for any special or consequential damages that result from the use of, or the inability to use, the materials on this site or the performance of the products,
Exceptions include international sales and items that are agreed to be sold with a lesser warranty or items that are agreed to be sold “as is.”
Should there be visible shipping damage to packaging or concealed shipping damage to item(s) supplied, buyer must communicate such damage to the delivering entity or carrier upon receipt first and to American Instrument the same day item is accepted / signed for. Failure to do so voids warranty. Customer decision to involve / procure third party or OEM service other than American Instrument or AIE assigned vendors to resolve in-warranty instrument failure or defect without first placing a service call to American Instrument voids warranty.
Return & Cancellation Policy
American Instrument Exchange will authorize the return of and issue a full refund for any equipment that is not working as represented and could not be replaced or repaired to the customer’s satisfaction by AIE or a representative of AIE during the warranty period. Returns without prior authorization and an RMA number will not be accepted.
Customers are responsible for proper packaging of the equipment and all included accessories. American Instrument will pay for insurance and shipping costs. Non-inventory items bought specifically to fill customer orders may possibly be returned, but on a case by case basis.
Customers who cancel orders after refurbishing has begun or after delivery where there are no equipment performance issues may be subject to a Restocking/Cancellation Fee of 20% or more and responsible for shipping costs for both the delivery and return.
Ongoing and Extended Support Options
American Instrument Exchange is able to provide additional repair and support services outside the warranty terms for most products that sell.
For biosafety cabinets, clean benches, chemical fume hoods, and other clean air products our team at AIE Laboratory Services can provide certifications, repairs, decontaminations, and other services for customers throughout New England, and guidance and limited technical assistance for any customers in the US and Canada for new or refurbished biosafety cabinets purchased from American Instrument Exchange.
For all other products, the repair service team at American Instrument Exchange can provide both field and depot repair evaluations. We can easily schedule and coordinate the pick-up and transportation of your malfunctioning equipment. By offering hourly rates lower than most OEM service providers and eliminating travel time for technicians, we help reduce repair costs. Furthermore, our lab depot repair service minimizes or eliminates scheduling delays. Our experienced service technicians are qualified to restore your equipment to factory specifications.
Yes, all equipment from American Instrument Exchange comes with a warranty. Used/reconditioned/refurbished equipment is tested to make sure it operates to the manufacturer's specifications and comes with a 90-day warranty (unless otherwise noted). New equipment comes with a warranty from the manufacturer.
How do you test and recondition the equipment?
Testing requirements vary across all the equipment categories we sell. Temperature controlled units are tested with NIST traceable probes and data loggers. Gaskets, external housing, glass, handles, controls and control screens are all thoroughly inspected for integrity and functionality. We test all equipment using the manufacturer's parameters and specifications to confirm that the unit operates properly.
How do products ship from American Instrument Exchange?
We can ship products both nationally and internationally using a well-established network of transporters. Smaller items will ship via UPS. Larger equipment will be palletized and shipped either LTL or air-ride, depending on the specific equipment and/or delivery requirements.
Return and order cancellation policy
American Instrument Exchange will accept the return of any product that is not working as represented and could not be replaced or repaired to the customer's satisfaction during the warranty period. Customers who cancel orders after refurbishing has begun will be subject to a cancellation and restocking fee of between 20% and 40%.
Are discounts available for educational or non-profit institutions, or for bulk purchases?
Yes – call or email us today with a list of equipment you're looking for and we can potentially put together a quote that includes a discount.
How does your price matching policy work?
We strive to keep our pricing competitive and reasonable. If you find comparable items for sale at a lower price from other vendors please send us a link and or quote for the item and we'll do our best to match or beat the price.
Can I see the equipment in person before committing to a purchase?
Customers are welcome to make an appointment to view equipment in our warehouse. Given our workload we may not be able to test or clean the equipment prior to your arrival if you have not committed to an order.
1023 Western Avenue is a working warehouse, not a showroom, and customers should dress appropriately and wear closed toe shoes.
Can I ship products that I purchased elsewhere to American Instrument for repair?
Yes – please see our repair services page for more information.
