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Fisher Isotemp Hybridization Oven
The Fisher Isotemp Hybridization Oven is a compact temperature-controlled laboratory oven designed specifically for nucleic acid and protein hybridization procedures. Combining precise temperature control with a variable-speed rotating bottle system, the Fisher Isotemp Hybridization Oven provides continuous sample movement throughout incubation while eliminating the need for many traditional water-bath-based hybridization methods.
Available used and refurbished from American Instrument Exchange, this Fisher Isotemp hybridization oven is particularly well suited for molecular biology, genetics, biotechnology, pharmaceutical research, and academic laboratories performing Southern blots, Northern blots, Western blot procedures, and other membrane-based hybridization protocols. Its compact benchtop design provides dedicated hybridization capability without occupying the space required by a larger general-purpose incubator or shaking water bath.
Key Features
Designed specifically for nucleic acid and protein hybridization
Temperature range from approximately ambient +7°C to 85°C
Variable-speed rotisserie from approximately 5 to 15 RPM
Approximately ±0.5°C chamber temperature uniformity at 55°C
Approximately ±0.24°C sample-to-sample uniformity at 55°C
Approximately 20-minute heat-up time from ambient to 65°C
Digital menu-driven temperature and rotisserie controls
Rotating bottle system provides continuous sample movement
Compact benchtop configuration
Chamber dimensions approximately 13"W × 8"D × 8"H
120V, 60 Hz electrical configuration
Professionally inspected and temperature tested by American Instrument Exchange
90-day warranty included
Fisher Isotemp Hybridization Oven Overview
The Fisher Isotemp Hybridization Oven was developed specifically around the requirements of membrane hybridization rather than general-purpose laboratory incubation. Its combination of controlled heat and continuous rotation creates an environment in which hybridization membranes can remain evenly exposed to hybridization solution throughout an extended procedure.
The central feature of the system is its variable-speed rotisserie. Hybridization bottles containing membranes and solution are mounted horizontally on the rotating assembly, which turns continuously at approximately 5 to 15 RPM. As each bottle rotates, hybridization solution repeatedly flows across the membrane surface.
This approach provides continuous mixing without requiring large quantities of hybridization solution. It also eliminates the need to place bottles or sealed bags inside a shaking water bath, simplifying the workflow while keeping the hybridization process contained inside a dedicated benchtop instrument.
Temperature can be controlled from approximately 7°C above ambient to 85°C, providing the range required for many nucleic acid and protein hybridization procedures. Digital menu-driven controls allow researchers to establish both chamber temperature and rotisserie speed according to the requirements of the protocol.
With exterior dimensions of approximately 18"W × 16.5"D × 17"H, the Fisher Isotemp Hybridization Oven requires relatively little laboratory bench space and can remain available as a dedicated instrument for blotting and molecular biology workflows.
Common Applications
The Fisher Isotemp Hybridization Oven is particularly well suited for molecular biology, genetics, genomics, biotechnology, pharmaceutical research, clinical research, and academic laboratories performing membrane-based detection procedures.
Typical applications include Southern blot hybridization for DNA analysis, Northern blot hybridization for RNA analysis, protein blotting procedures, membrane washing, probe hybridization, and other temperature-controlled molecular biology protocols.
The rotating bottle format is especially useful for procedures requiring membranes to remain continuously exposed to hybridization or wash solutions over extended periods. Continuous rotation helps distribute solution across the membrane while reducing the volume of reagent needed compared with some open-tray or water-bath methods.
Molecular biology laboratories may use the Fisher Isotemp Hybridization Oven alongside laboratory centrifuges for sample preparation and nucleic acid processing, laboratory refrigerators for reagent storage, and laboratory freezers for longer-term storage of biological samples and molecular biology reagents.
Samples, Membranes & Hybridization Applications
Unlike a conventional incubator designed primarily around flasks, dishes, or sample containers, the Fisher Isotemp Hybridization Oven is optimized for membranes contained inside rotating hybridization bottles.
Common Samples & Applications
DNA blots
RNA blots
Protein blots
Southern blot membranes
Northern blot membranes
Membrane-bound nucleic acids
Membrane-bound proteins
Labeled probe hybridization
Typical Hybridization Materials
Glass hybridization bottles
Nylon membranes
Nitrocellulose membranes
Supported nitrocellulose membranes
Hybridization buffers
Wash solutions
Labeled DNA or RNA probes
Protein detection reagents
Bottle rotation provides continuous contact between the membrane and hybridization solution. This can improve consistency while allowing researchers to use relatively small reagent volumes compared with procedures that require membranes to be completely submerged in larger trays or baths.
The appropriate temperature, rotation speed, bottle size, hybridization solution, and incubation duration depend on the membrane, probe, detection chemistry, and individual laboratory protocol.
Temperature Control & Hybridization Performance
Precise temperature control is particularly important during nucleic acid hybridization because incubation temperature influences hybridization stringency and probe binding. The Fisher Isotemp Hybridization Oven combines controlled heating with continuous bottle rotation to maintain repeatable conditions throughout the procedure.
The chamber operates from approximately ambient +7°C to 85°C, providing sufficient range for many commonly used hybridization and membrane-washing protocols.
Published performance specifications indicate chamber temperature uniformity of approximately ±0.5°C at 55°C, with sample-to-sample uniformity of approximately ±0.24°C at the same setpoint. Consistent temperature between bottles helps researchers maintain comparable hybridization conditions when several samples are processed simultaneously.
The oven can heat from ambient conditions to approximately 65°C in 20 minutes, reducing the amount of preparation time required before beginning a temperature-sensitive procedure.
The rotisserie operates from approximately 5 to 15 RPM, allowing researchers to adjust bottle movement according to the requirements of the protocol. Continuous rotation repeatedly coats the membrane with hybridization solution while helping maintain mixing throughout the incubation period.
This combination of precise temperature control, continuous rotation, compact chamber size, and dedicated hybridization-bottle handling makes the Fisher Isotemp a more purpose-built solution for blotting workflows than a conventional laboratory incubator.
Fisher Isotemp Hybridization Oven Specifications
Specification
Details
Manufacturer
Fisher Scientific
Product Series
Isotemp
Equipment Type
Hybridization Oven / Rotisserie Incubator
Primary Application
Nucleic acid and protein hybridization
Temperature Range
Ambient +7°C to 85°C
Temperature Uniformity
±0.5°C at 55°C within chamber
Sample-to-Sample Uniformity
±0.24°C at 55°C
Heat-Up Time
Approximately 20 minutes from ambient to 65°C
Rotisserie Speed
Approximately 5–15 RPM
Rotation
Variable speed
Temperature Control
Digital
Control Interface
Menu-driven
Refrigeration
No
CO₂ Control
No
Active Humidity Control
No
Interior Dimensions (W × D × H)
Approximately 13" × 8" × 8"
Exterior Dimensions (W × D × H)
Approximately 18" × 16.5" × 17"
Electrical Requirements
120V, 60 Hz
Configuration
Benchtop
Specifications and included rotisserie components can vary according to production date and individual unit configuration. Confirm bottle capacity, rotisserie configuration, accessories, electrical requirements, and included hybridization bottles for the individual unit before purchase.
A hybridization oven provides controlled temperature and continuous membrane agitation for nucleic acid and protein hybridization procedures. Common applications include Southern blots, Northern blots, membrane washing, probe hybridization, and related molecular biology procedures.
What is the advantage of a rotating hybridization oven?
The rotisserie continuously rotates bottles containing membranes and hybridization solution. This keeps the membrane exposed to moving solution throughout the procedure while allowing researchers to use relatively small reagent volumes compared with some open-tray or water-bath methods.
What is the temperature range of the Fisher Isotemp Hybridization Oven?
The Fisher Isotemp Hybridization Oven operates from approximately 7°C above ambient to 85°C, providing a broad range for nucleic acid and protein hybridization as well as membrane-washing procedures.
How fast does the rotisserie rotate?
Rotisserie speed is adjustable from approximately 5 to 15 RPM. Variable speed allows researchers to select an appropriate rotation rate for the bottles, membranes, and hybridization protocol being used.
Can the Fisher Isotemp Hybridization Oven replace a shaking water bath?
For many membrane hybridization procedures, yes. A dedicated hybridization oven provides controlled heating and continuous movement without requiring hybridization bottles or bags to be immersed in a water bath. The appropriate equipment ultimately depends on the specific laboratory protocol.
Is this a general-purpose laboratory incubator?
The Fisher Isotemp Hybridization Oven can provide controlled elevated temperatures, but it is specifically designed for hybridization bottles and molecular biology workflows. Laboratories primarily incubating cultures, plates, or general laboratory samples will usually be better served by a conventional laboratory incubator.
Is this Fisher Isotemp Hybridization Oven refurbished?
Used Fisher Isotemp Hybridization Ovens offered by American Instrument Exchange are inspected, cleaned, and tested prior to shipment. Temperature performance and the accuracy of the controls and readouts are evaluated using calibrated temperature-monitoring equipment, while the rotisserie drive, speed control, door, safety systems, and other installed functions are tested as applicable. Refurbished equipment includes a 90-day warranty.
Click Hereto see the Fisher Isotemp Hybridization Incubator on the American Instrument Exchange YouTube channel.
Testing Procedures for Used and Refurbished Laboratory Incubators Ensure Quality and Stability Before Use
Used and refurbished laboratory incubators from American Instrument Exchange are thoroughly tested to ensure optimal performance and temperature stability with laboratory safety standards. Each unit undergoes a detailed inspection and functionality assessment to meet the needs of research, clinical, and industrial labs.
Why Testing Used Laboratory Incubators Is Essential
Before a laboratory incubator is reintroduced into any work environment, it must be tested for:
• Temperature uniformity and accuracy
• Airflow and humidity control (if applicable)
• Door seal integrity and insulation effectiveness
• Electrical and safety system compliance
Comprehensive testing reduces the risk of culture loss, contamination, and equipment failure—especially critical in applications involving cell culture, microbiology, pharmaceutical research, and diagnostics.
Our multi-point testing protocol for used and refurbished incubators ensures reliability, safety, and performance across all models and brands.
Key Testing Procedures for Refurbished and Used Laboratory Incubators
1. Initial Visual Inspection
• Check for cosmetic damage, corrosion, and wear to exterior surfaces, door hinges, and gasket seals
• Inspect power cord, wiring, and display panel for any defects or signs of tampering
• Confirm that shelves, chamber components, and internal fixtures are present and undamaged
2. Functional Testing
• Power on the incubator and verify all display indicators and alarms function correctly
• Test door latch and seal mechanisms for proper closure and insulation
• Run basic temperature cycles to ensure the unit maintains stability over time
3. Temperature Accuracy and Uniformity Tests
• Test internal sensors and controllers using temperature probes
• Measure temperature consistency at multiple chamber locations over several hours
• Confirm that programmable temperature settings respond accurately
4. Airflow and Humidity Control (if applicable)
• Test internal fan operation and airflow distribution
• For humidified incubators, inspect and test humidity reservoirs, sensors, and control systems
• Check for condensation buildup and confirm environmental stability
5. Electrical Safety and Compliance Checks
• Ensure all electrical components are properly grounded
• Perform insulation resistance and leakage current tests
• Verify compliance with current electrical safety standards for laboratory equipment
6. Final Quality Assurance Checklist
• Perform full cycle testing at various temperature setpoints
• Document performance benchmarks and results
All refurbished incubators from American Instrument Exchange are backed by our commitment to quality and precision. With our strict testing procedures, you can rely on your incubator to deliver dependable results from day one.
Repair and Refurbishment Protocol for Used Laboratory Incubators Performance and Quality from American Instrument Exchange
At American Instrument Exchange, every used laboratory incubator we sell goes through a repair and refurbishment protocol. Whether it’s a standard, CO₂, or refrigerated incubator, our goal is to restore each unit to laboratory-level performance—ensuring temperature accuracy, environmental stability, and long-term reliability in your lab.
Why Choose a Refurbished Laboratory Incubator from American Instrument Exchange?
• Fully tested for performance and safety
• Backed by a warranty
• Cost-effective alternative to new equipment
• Environmentally sustainable through reuse and restoration
• Refurbished by expert technicians with decades of lab equipment experience
Step-by-Step Refurbishment Protocol
Every incubator at American Instrument Exchange follows this comprehensive, multi-stage process:
1. Initial Intake & Inspection
• Visual inspection for cosmetic wear, corrosion, dents, or broken components
• Mechanical assessment of door hinges, gaskets, seals, and chamber components
• Document any missing parts or issues requiring repair or replacement
2. Cleaning and Decontamination
• Thorough internal and external cleaning using non-abrasive solutions
• Decontamination/cleaning of surfaces to remove biological, chemical, or particulate residue
• Cleaning of removable parts (e.g., shelves, trays, etc.)
3. Mechanical and Electrical Repair
• Repair or replacement of worn mechanical components such as door latches, fans, hinges, and seals
• Evaluation and repair of control panels, switches, thermostats, and displays
• Inspection and testing of wiring, fuses, relays, and power supplies
4. Environmental Control System Assessment
• For CO₂ incubators: CO₂ sensors, regulators, and gas delivery systems are tested
• For refrigerated incubators: compressors, condensers, and cooling systems are evaluated for performance and leaks
• Fan motors and airflow systems are tested to ensure uniform environmental conditions
5. Temperature and Sensor Testing
• All temperature sensors and controllers are tested using thermometers/probes
• Uniformity and accuracy tested at multiple chamber locations
• For humidified or CO₂ incubators, humidity and CO₂ stability are verified
6. Safety System Testing
• Test of over-temperature protection systems and alarms
• Evaluation of door sensors, safety interlocks, and power failure recovery
7. Final Quality Control and Documentation
• Full performance test run over 24 hours
• Validation of setpoints for temperature, CO₂ (if applicable), and humidity (if applicable)
• Final inspection and cleaning before packaging
• Complete service report
Trust the Experts at American Instrument Exchange
With over 50 years in the lab equipment industry, American Instrument Exchange is a trusted source for high-quality refurbished laboratory incubators. Our proven refurbishment protocol ensures every unit is reliable, accurate, and ready to meet the demands of your lab from day one.
Warranty Information
All used equipment sold by American Instrument Exchange is refurbished/reconditioned, tested to meet specifications, cleaned, and comes with a 90-day warranty from the date of shipment unless otherwise specified. New products include a warranty from the manufacturer. Your warranty is only valid if you have paid your invoice. If your invoice is over 30 days old, warranty work will not be performed until your invoice is paid in full. American Instrument Exchange is not liable for any special or consequential damages that result from the use of, or the inability to use, the materials on this site or the performance of the products,
Exceptions include international sales and items that are agreed to be sold with a lesser warranty or items that are agreed to be sold “as is.”
Should there be visible shipping damage to packaging or concealed shipping damage to item(s) supplied, buyer must communicate such damage to the delivering entity or carrier upon receipt first and to American Instrument the same day item is accepted / signed for. Failure to do so voids warranty. Customer decision to involve / procure third party or OEM service other than American Instrument or AIE assigned vendors to resolve in-warranty instrument failure or defect without first placing a service call to American Instrument voids warranty.
Return & Cancellation Policy
American Instrument Exchange will authorize the return of and issue a full refund for any equipment that is not working as represented and could not be replaced or repaired to the customer’s satisfaction by AIE or a representative of AIE during the warranty period. Returns without prior authorization and an RMA number will not be accepted.
Customers are responsible for proper packaging of the equipment and all included accessories. American Instrument will pay for insurance and shipping costs. Non-inventory items bought specifically to fill customer orders may possibly be returned, but on a case by case basis.
Customers who cancel orders after refurbishing has begun or after delivery where there are no equipment performance issues may be subject to a Restocking/Cancellation Fee of 20% or more and responsible for shipping costs for both the delivery and return.
Ongoing and Extended Support Options
American Instrument Exchange is able to provide additional repair and support services outside the warranty terms for most products that sell.
For biosafety cabinets, clean benches, chemical fume hoods, and other clean air products our team at AIE Laboratory Services can provide certifications, repairs, decontaminations, and other services for customers throughout New England, and guidance and limited technical assistance for any customers in the US and Canada for new or refurbished biosafety cabinets purchased from American Instrument Exchange.
For all other products, the repair service team at American Instrument Exchange can provide both field and depot repair evaluations. We can easily schedule and coordinate the pick-up and transportation of your malfunctioning equipment. By offering hourly rates lower than most OEM service providers and eliminating travel time for technicians, we help reduce repair costs. Furthermore, our lab depot repair service minimizes or eliminates scheduling delays. Our experienced service technicians are qualified to restore your equipment to factory specifications.
Yes, all equipment from American Instrument Exchange comes with a warranty. Used/reconditioned/refurbished equipment is tested to make sure it operates to the manufacturer's specifications and comes with a 90-day warranty (unless otherwise noted). New equipment comes with a warranty from the manufacturer.
How do you test and recondition the equipment?
Testing requirements vary across all the equipment categories we sell. Temperature controlled units are tested with NIST traceable probes and data loggers. Gaskets, external housing, glass, handles, controls and control screens are all thoroughly inspected for integrity and functionality. We test all equipment using the manufacturer's parameters and specifications to confirm that the unit operates properly.
How do products ship from American Instrument Exchange?
We can ship products both nationally and internationally using a well-established network of transporters. Smaller items will ship via UPS. Larger equipment will be palletized and shipped either LTL or air-ride, depending on the specific equipment and/or delivery requirements.
Return and order cancellation policy
American Instrument Exchange will accept the return of any product that is not working as represented and could not be replaced or repaired to the customer's satisfaction during the warranty period. Customers who cancel orders after refurbishing has begun will be subject to a cancellation and restocking fee of between 20% and 40%.
Are discounts available for educational or non-profit institutions, or for bulk purchases?
Yes – call or email us today with a list of equipment you're looking for and we can potentially put together a quote that includes a discount.
How does your price matching policy work?
We strive to keep our pricing competitive and reasonable. If you find comparable items for sale at a lower price from other vendors please send us a link and or quote for the item and we'll do our best to match or beat the price.
Can I see the equipment in person before committing to a purchase?
Customers are welcome to make an appointment to view equipment in our warehouse. Given our workload we may not be able to test or clean the equipment prior to your arrival if you have not committed to an order.
1023 Western Avenue is a working warehouse, not a showroom, and customers should dress appropriately and wear closed toe shoes.
Can I ship products that I purchased elsewhere to American Instrument for repair?
Yes – please see our repair services page for more information.
