Thermo Scientific Heracell 150i Copper Tri-Gas CO₂ Incubator – Refurbished
The Thermo Scientific Heracell 150i Copper Tri-Gas CO₂ Incubator is a premium laboratory cell culture incubator designed to provide precise temperature control, a stable CO₂ atmosphere, elevated humidity, and enhanced protection against microbial contamination. Featuring a 150 L (5.3 cu. ft.) copper-lined chamber, direct-heat air-jacket technology, integrated humidification, and Thermo Scientific's ContraCon moist-heat decontamination system, the Heracell 150i is particularly well suited for biotechnology, pharmaceutical research, stem cell culture, tissue engineering, and other demanding biological applications.
Available used and refurbished from American Instrument Exchange, this Thermo Heracell 150i Copper offers laboratories a cost-effective alternative to purchasing a new high-performance cell culture incubator. Its antimicrobial copper surfaces, sophisticated environmental monitoring, and contamination-control features make it an excellent choice for laboratories seeking dependable incubation conditions while protecting valuable cell cultures.
Key Features
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150 L (5.3 cu. ft.) incubation chamber
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Copper-lined interior with naturally antimicrobial surfaces
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Tri-gas configuration as identified by American Instrument Exchange
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Direct-heat, air-jacketed temperature control
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Temperature range from ambient +3°C to 55°C
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CO₂ control range from 0% to 20%
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Thermal conductivity (TC) CO₂ sensor
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Integrated heated water reservoir for humidification
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High-humidity environment approaching 95% RH under specified conditions
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Gentle fan-assisted air circulation
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ContraCon 90°C moist-heat decontamination cycle
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iCAN touchscreen controller with performance trend monitoring
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Dual temperature probes and overtemperature protection
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Heated inner glass observation door
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RS-232 communications interface
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Adjustable shelving for flexible sample organization
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Refurbished and tested by American Instrument Exchange
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90-day warranty included
Thermo Scientific Heracell 150i Copper Overview
The Thermo Heracell 150i Copper was developed for laboratories that require reliable cell culture conditions while minimizing the risks associated with microbial contamination.
Its 150 L chamber provides sufficient capacity for routine mammalian cell culture, stem cell research, and biotechnology applications without occupying the substantial floor space required by larger reach-in incubators.
The copper-lined chamber is one of the incubator's most important distinguishing characteristics. Copper possesses naturally antimicrobial properties that can reduce the survival of many microorganisms on exposed surfaces. This provides an additional contamination-control measure during routine laboratory operation.
The Heracell 150i also incorporates a direct-heat air-jacketed temperature-control system. Heating elements maintain the surrounding air jacket at the appropriate temperature, while gentle internal air circulation helps distribute conditioned air throughout the chamber.
An integrated heated water reservoir provides elevated humidity, helping prevent excessive evaporation from culture media during extended incubation procedures.
The incubator's iCAN touchscreen controller provides access to temperature and CO₂ settings, performance monitoring, alarm information, and operating logs.
Together, these features make the Heracell 150i Copper an attractive option for laboratories maintaining sensitive or valuable biological cultures.
Common Applications
The Thermo Heracell 150i Copper is suitable for a wide range of mammalian cell culture and biotechnology applications, including stem cell research, primary cell culture, pharmaceutical development, tissue engineering, regenerative medicine, antibody production, and vaccine research.
In academic and biotechnology laboratories, the incubator provides a controlled environment for routine cell line maintenance and experimental culture procedures.
Pharmaceutical laboratories may use the Heracell 150i for drug discovery, cell-based assays, toxicology studies, and biological research requiring consistent incubation conditions.
The copper chamber and ContraCon decontamination system are particularly attractive for laboratories working with sensitive cell lines or expensive cultures where contamination can result in substantial losses.
For laboratories performing aseptic cell culture, biosafety cabinets provide appropriate workspaces for handling cells and culture media. Laboratory centrifuges support cell harvesting and sample preparation, while laboratory refrigerators and ultra-low temperature freezers provide complementary storage for reagents and biological materials.
Cell Types & Culture Applications
The Heracell 150i Copper is designed for CO₂-dependent cell culture procedures requiring stable temperature, atmospheric conditions, and elevated humidity.
Typical applications include:
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CHO and HEK293 cell cultures
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Stem cells and progenitor cells
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Primary mammalian cell cultures
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Fibroblast cultures
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Epithelial and endothelial cells
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Hybridoma cultures
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Cell-based pharmaceutical assays
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Tissue engineering and regenerative medicine
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Vaccine and antibody research
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Long-term cell culture experiments
The chamber accommodates standard cell culture flasks, dishes, multiwell plates, bottles, and other vessels used in mammalian cell culture.
The incubator's high-humidity environment helps reduce evaporation from culture media, supporting consistent concentrations of dissolved nutrients and other media components during extended incubation.
Because this unit is identified as a tri-gas configuration, it may also be suitable for applications requiring oxygen regulation, provided the installed oxygen-control hardware and operating range are verified.
Temperature, CO₂ & Environmental Stability
The Thermo Heracell 150i Copper combines direct-heat temperature control, thermal conductivity CO₂ sensing, gentle chamber circulation, and integrated humidification to maintain stable culture conditions.
Its operating temperature range extends from approximately 3°C above ambient to 55°C, accommodating conventional mammalian cell culture at 37°C and other temperature-controlled biological procedures.
The direct-heat air-jacket system distributes heat around the chamber, while a low-velocity internal fan promotes consistent temperature and atmospheric conditions throughout the interior.
The CO₂ concentration is adjustable from 0% to 20%. This particular Heracell 150i uses a thermal conductivity sensor rather than the infrared sensor available in certain other configurations.
Thermal conductivity sensing measures changes in the thermal properties of the chamber atmosphere to determine CO₂ concentration. Because temperature and humidity can influence these measurements, maintaining stable environmental conditions and proper sensor calibration is important.
Dual temperature probes provide additional monitoring and support overtemperature protection.
The iCAN controller displays chamber conditions and allows operators to review performance trends and alarm information, helping identify deviations during extended incubation procedures.
Integrated Humidification & Rapid Recovery
Maintaining elevated humidity is essential for many mammalian cell culture applications because evaporation can alter media composition and affect cell growth.
The Heracell 150i incorporates a heated water reservoir designed to maintain a high-humidity chamber environment.
Unlike conventional incubators that rely on passive evaporation from a removable water pan, the integrated heated reservoir supports rapid humidity recovery following routine door openings.
Thermo's design is intended to restore humidity substantially faster than traditional passive humidification methods under comparable operating conditions.
This can be particularly beneficial in busy cell culture laboratories where multiple researchers access the incubator throughout the day.
The heated inner glass door also helps minimize condensation while allowing researchers to inspect cultures without fully opening the chamber.
The reservoir should be maintained with water meeting the manufacturer's requirements, and routine cleaning remains important for minimizing contamination.
Copper Chamber & ContraCon Decontamination
The copper-lined chamber is a defining feature of this Heracell 150i configuration.
Copper possesses natural antimicrobial properties that can inhibit the survival of many bacteria and other microorganisms on exposed surfaces.
For laboratories working with valuable or contamination-sensitive cultures, these surfaces provide an additional protective measure alongside routine cleaning and aseptic technique.
The Heracell 150i also incorporates Thermo Scientific's ContraCon moist-heat decontamination system.
ContraCon uses a controlled 90°C moist-heat cycle designed to reduce microbial contamination throughout the chamber. The process provides an automated alternative to relying exclusively on manual chemical disinfection.
The combination of copper surfaces and ContraCon decontamination is particularly valuable for laboratories performing repeated culture experiments, maintaining sensitive cell lines, or operating multiple incubators with different biological materials.
Copper surfaces do not eliminate the need for cleaning, and the ContraCon cycle should be performed according to the manufacturer's instructions.
iCAN Controller & Monitoring
The Heracell 150i features Thermo Scientific's iCAN touchscreen controller, providing centralized access to operating settings and environmental monitoring information.
The controller displays temperature and CO₂ conditions and incorporates performance trend graphing, error logs, and usage information.
These features allow laboratory personnel to review incubation performance and identify changes that could affect culture conditions.
The incubator also incorporates alarms and protective controls to alert users to certain operating deviations.
An RS-232 communications interface allows connection to compatible external monitoring systems, providing additional flexibility for laboratories requiring independent data collection.
Thermo Scientific Heracell 150i Copper Specifications
| Specification |
Details |
| Manufacturer |
Thermo Scientific |
| Model |
Heracell 150i Copper |
| AIE Product Code |
3180RR INC |
| Equipment Type |
Copper-Lined Tri-Gas CO₂ Incubator |
| Chamber Capacity |
150 L (5.3 cu. ft.) |
| Chamber Material |
Copper-lined interior |
| Heating System |
Direct heat / air jacket |
| Temperature Range |
Ambient +3°C to 55°C |
| Temperature Stability |
Approximately ±0.1°C under specified conditions |
| Temperature Uniformity |
Approximately ±0.3°C at 37°C, configuration-dependent |
| CO₂ Range |
0–20% |
| CO₂ Sensor |
Thermal conductivity (TC) |
| Oxygen Control |
Tri-gas configuration listed; installed sensor and range to be verified |
| Humidification |
Integrated heated water reservoir |
| Relative Humidity |
Up to approximately 95% RH at 37°C |
| Air Circulation |
Gentle fan-assisted circulation |
| Decontamination |
ContraCon moist-heat cycle |
| Decontamination Temperature |
90°C |
| Controller |
iCAN touchscreen |
| Inner Door |
Heated glass |
| Standard Shelves |
3 |
| Maximum Shelves |
Up to 10 |
| Interior Dimensions (W × D × H) |
Approximately 20.9" × 21.7" × 24.8" |
| Exterior Dimensions (W × D × H) |
Approximately 27.6" × 31.5" × 38.2" |
| Electrical Requirements |
120V, 50/60 Hz; verify nameplate |
| Communication Interface |
RS-232 |
| Warranty |
90 days through American Instrument Exchange |
Specifications are based on the Heracell 150i Copper platform and the AIE listing for SKU 3180RR INC. Oxygen-control hardware, sensor type, range, electrical configuration, dimensions, and included accessories should be verified for the individual refurbished unit.
Thermo Scientific Heracell 150i Copper Frequently Asked Questions
What is the advantage of a copper-lined CO₂ incubator?
Copper has naturally antimicrobial properties that can reduce the survival of many microorganisms on exposed chamber surfaces. The Heracell 150i Copper incorporates these surfaces as an additional contamination-control measure, helping protect sensitive cultures when used alongside appropriate aseptic procedures.
Is this Heracell 150i a tri-gas incubator?
American Instrument Exchange identifies this particular unit as a tri-gas incubator. Tri-gas systems are designed to regulate temperature, CO₂, and oxygen when equipped with the appropriate controls and gas connections. The installed oxygen sensor and operating range should be verified before purchase.
What type of CO₂ sensor does this unit use?
This Heracell 150i uses a thermal conductivity CO₂ sensor with a 0–20% control range. Other Heracell configurations may incorporate infrared CO₂ sensors, so it is important to distinguish between individual units.
Is the Heracell 150i Copper suitable for stem cell culture?
Yes. The incubator provides controlled temperature, CO₂ concentration, and elevated humidity for many stem cell culture applications. Its copper-lined chamber and ContraCon decontamination system are particularly useful for laboratories maintaining contamination-sensitive cultures.
How does the ContraCon decontamination system work?
ContraCon uses a controlled 90°C moist-heat decontamination cycle designed to reduce microbial contamination within the incubator chamber. The system helps simplify routine contamination-control procedures and reduces reliance on chemical disinfection alone.
Does the Heracell 150i control relative humidity?
The incubator uses an integrated heated water reservoir to maintain elevated humidity, approaching approximately 95% RH under specified conditions. It is not a general-purpose programmable relative-humidity chamber with independently adjustable RH setpoints.
How many shelves can the Heracell 150i accommodate?
The Heracell 150i platform normally includes three shelves and can accommodate up to ten, depending on the configuration. The number of shelves included with this individual refurbished unit should be confirmed before purchase.
What is the difference between a copper Heracell 150i and a stainless steel model?
Both versions provide controlled temperature, CO₂ concentration, and humidity for cell culture. The primary distinction is the chamber material. The copper version provides naturally antimicrobial interior surfaces, while stainless steel versions use conventional corrosion-resistant chamber construction.
Is this Thermo Heracell 150i Copper refurbished?
This Thermo Scientific Heracell 150i Copper is offered used and refurbished by American Instrument Exchange. AIE technicians inspect, clean, and test incubators to verify temperature performance, CO₂ control, humidification, and other installed functions as applicable. Components are repaired or replaced as necessary, and refurbished equipment includes a 90-day warranty.