Eppendorf 5810 Benchtop Centrifuge

Eppendorf 5810 Benchtop Centrifuge with A-4-62 Swing-Bucket Rotor

Not every laboratory needs refrigerated centrifugation. For routine cell culture, clinical research, sample preparation, blood tube processing, and general biotechnology workflows where temperature control is not required, eliminating refrigeration can mean a simpler instrument, a smaller footprint, and less equipment to maintain.

The Eppendorf 5810 Benchtop Centrifuge with A-4-62 swing-bucket rotor combines the versatility of Eppendorf's popular 58xx centrifuge platform with a highly adaptable general-purpose rotor. This particular unit includes the A-4-62 rotor with four 250 mL rectangular buckets, providing a maximum rotor speed of 4,000 RPM and 3,220 × g. With appropriate adapters, the rotor can accommodate laboratory vessels ranging from small tubes through 250 mL bottles, making this configuration particularly useful in laboratories that regularly switch between different sample formats.

Although the included A-4-62 is designed for medium-speed, higher-capacity processing, the Eppendorf 5810 itself is capable of substantially greater performance when equipped with compatible fixed-angle rotors. The platform can reach 14,000 RPM and 20,913 × g, allowing laboratories to expand the centrifuge's capabilities if their workflows change.

At American Instrument Exchange, each refurbished centrifuge is professionally inspected, cleaned, and tested before shipment. Our technicians evaluate the drive system, bearings, braking, controls, lid and safety mechanisms, and overall mechanical performance. Because this centrifuge includes an A-4-62 rotor, the centrifuge and rotor are tested together as a complete system prior to shipment.

Features of the Eppendorf 5810 with A-4-62 Rotor

• Includes Eppendorf A-4-62 swing-bucket rotor

• Includes four 250 mL rectangular buckets

• Maximum speed with A-4-62 rotor: 4,000 RPM

• Maximum RCF with A-4-62 rotor: 3,220 × g

• Maximum A-4-62 capacity: 4 × 250 mL

• Accommodates tubes and bottles from approximately 0.2 mL through 250 mL with appropriate adapters

• Plate-processing capability with appropriate A-4-62 plate buckets

• Centrifuge platform capable of up to 14,000 RPM and 20,913 × g, rotor dependent

• Non-refrigerated design

• Automatic rotor recognition

• Imbalance detection

• 35 user-defined program spaces

• 10 acceleration and 10 braking ramps

• Low access height for comfortable sample loading

• Compact footprint for a high-capacity multipurpose centrifuge

• 120V, 50–60 Hz electrical configuration

• 90-day warranty included from American Instrument Exchange

• Refurbished and tested by American Instrument Exchange

For laboratories that do not routinely centrifuge temperature-sensitive samples, the non-refrigerated 5810 provides much of the versatility of the 5810R while eliminating the compressor and refrigeration system.

Why Choose the Non-Refrigerated Eppendorf 5810?

The decision between an Eppendorf 5810 and 5810R should largely come down to the samples being processed.

If a protocol requires samples to remain chilled during centrifugation, the refrigerated 5810R is the appropriate choice. But many routine centrifugation procedures are performed at ambient temperature. In those situations, refrigeration may add cost, weight, power consumption, and maintenance requirements without providing a meaningful benefit to the workflow.

The 5810 weighs approximately 150 pounds, compared with roughly 218 pounds for the refrigerated 5810R. Its compact benchtop design and lack of a refrigeration system can make it easier to install in laboratories where space and equipment mobility matter.

Importantly, choosing the non-refrigerated model does not mean giving up the broader versatility of the 5810 platform. Compatible swing-bucket, fixed-angle, and plate rotors allow the centrifuge to support applications ranging from routine tube processing to high-speed molecular biology.

For laboratories comparing different configurations, browse American Instrument Exchange's selection of used and refurbished laboratory centrifuges from Eppendorf, Thermo Scientific, Beckman Coulter, and other manufacturers.

Common Applications for the Eppendorf 5810 with A-4-62 Rotor

The A-4-62 configuration is especially useful for laboratories that process multiple vessel formats at moderate centrifugal forces.

Common applications include:

• Routine mammalian cell culture processing

• Cell harvesting and pelleting

• 15 mL and 50 mL conical tube processing

• Blood collection tube centrifugation

• Clinical and translational research

• Bacterial culture processing

• General biotechnology workflows

• Sample clarification

• Molecular biology sample preparation

• Pharmaceutical research

• Buffer and reagent preparation

• Plate processing with appropriate buckets

For laboratories running conical tubes, the appropriate A-4-62 adapters can accommodate multiple tubes per bucket, allowing considerably more samples to be processed during each run than with many smaller general-purpose centrifuges.

Eppendorf A-4-62 Rotor and Included Accessories

This particular Eppendorf 5810 includes the A-4-62 swing-bucket rotor with four 250 mL rectangular buckets.

The A-4-62 provides:

• 4 × 250 mL maximum bucket capacity

• 4,000 RPM maximum speed

• 3,220 × g maximum RCF

• Swinging-bucket geometry

• Compatibility with vessels ranging from approximately 0.2 mL through 250 mL with appropriate adapters

• Support for common conical and blood collection tube formats

• Optional aerosol-tight bucket caps

• Plate-processing capability using appropriate MTP buckets

With adapters for conical tubes, the 5810/A-4-62 combination can accommodate configurations of up to 36 × 15 mL or 16 × 50 mL conical tubes, making it a useful choice for laboratories processing multiple samples simultaneously.

One of the advantages of the A-4-62 is that the rotor does not lock the centrifuge into a single sample format. Changing adapters can allow the same rotor to support different tubes or bottles as laboratory workflows evolve.

Eppendorf 5810 Specifications

Specifications compiled from Eppendorf manufacturer literature, technical references, and American Instrument Exchange product information.

Power Requirements and Installation Considerations

The Eppendorf 5810 operates on 120V, 50–60 Hz electrical service, making installation relatively straightforward in many U.S. laboratories.

At approximately 150 pounds, the 5810 is considerably lighter than the refrigerated 5810R but still requires a sturdy, level laboratory bench or centrifuge stand. Laboratories should also account for approximately 31.5 inches of vertical clearance with the lid open when positioning the centrifuge underneath shelves or cabinetry.

Because the 5810 does not contain a refrigeration system, laboratories also avoid the ventilation considerations and additional refrigeration components associated with a refrigerated centrifuge.

American Instrument Exchange securely prepares centrifuges and included rotors for shipment to help protect the instrument, chamber, controls, and rotating components during transit.

Refurbished Eppendorf Centrifuges from American Instrument Exchange

The Eppendorf 5810 is a long-established laboratory centrifuge platform, making proper evaluation particularly important when purchasing a pre-owned instrument.

American Instrument Exchange technicians inspect and test refurbished centrifuges for drive performance, bearings, braking, speed control, lid operation, safety systems, electronic controls, and overall mechanical condition. Rotors are inspected for condition and proper operation, and when a centrifuge is sold with a rotor, the complete combination is tested together before shipment.

This particular system includes the Eppendorf A-4-62 swing-bucket rotor and four 250 mL buckets, providing a ready-to-configure platform for a wide range of routine laboratory applications.

Laboratories outfitting additional areas of their facility can also source professionally refurbished laboratory incubators, laboratory freezers and ultra-low freezers, and biosafety cabinets from American Instrument Exchange.

Eppendorf 5810 Service and Support

American Instrument Exchange and AIE Laboratory Services provide diagnostics, preventative maintenance, and repair support for centrifuges and other general laboratory equipment.

Because the 5810 is non-refrigerated, troubleshooting is focused primarily on the drive assembly, bearings, braking system, lid and safety mechanisms, electronic controls, rotor recognition, and other mechanical or electrical components rather than refrigeration.

For laboratories with an existing centrifuge experiencing problems, our technicians can help evaluate whether repair or replacement with another refurbished centrifuge is the more practical option.

Eppendorf 5810 with A-4-62 Rotor Frequently Asked Questions

What is the difference between the Eppendorf 5810 and 5810R?

The most important difference is refrigeration. The Eppendorf 5810 is a ventilated, non-refrigerated centrifuge, while the 5810R incorporates a refrigeration system for temperature-controlled centrifugation. Laboratories processing temperature-sensitive biological samples may prefer the 5810R, while the 5810 can be a simpler and more economical choice for routine ambient-temperature workflows.

Does this Eppendorf 5810 include the A-4-62 rotor?

Yes. This particular centrifuge includes the Eppendorf A-4-62 swing-bucket rotor with four 250 mL rectangular buckets. American Instrument Exchange tests the centrifuge and rotor together before shipment.

Can the A-4-62 centrifuge 15 mL and 50 mL conical tubes?

Yes. With the appropriate adapters, the A-4-62 can accommodate common conical tube formats. Configurations are available for processing up to 36 × 15 mL or 16 × 50 mL conical tubes per run.

When would I need the refrigerated 5810R instead?

The 5810R is preferable when protocols require samples to remain cold during centrifugation—for example, certain temperature-sensitive proteins, cells, enzymes, or other biological materials. If your validated protocol permits centrifugation at ambient temperature, the non-refrigerated 5810 may be entirely sufficient.

Can the Eppendorf 5810 process microplates?

Yes. The A-4-62 can be converted for plate processing using the appropriate MTP buckets, and the broader 5810 platform also supports dedicated plate rotor configurations. Laboratories planning to process plates should confirm the necessary rotor and bucket accessories before purchase.

Why is the centrifuge rated to 14,000 RPM if the included rotor only runs at 4,000 RPM?

The 14,000 RPM / 20,913 × g specification describes the maximum capability of the 5810 centrifuge platform with an appropriate high-speed rotor. The included A-4-62 is a larger swing-bucket rotor designed for capacity and versatility and is therefore limited to 4,000 RPM / 3,220 × g.

Is there an advantage to buying the 5810 instead of a smaller benchtop centrifuge?

For laboratories processing several different vessel types, there can be. The 5810 offers a larger rotor ecosystem and greater sample-format flexibility than many smaller centrifuges. A laboratory can use the A-4-62 for routine medium-capacity work and potentially add compatible rotors later if new applications require different capacities or higher speeds.

Is a refurbished Eppendorf 5810 still a good choice for a modern laboratory?

For laboratories that do not require refrigerated centrifugation or the newest instrument interface, a professionally refurbished 5810 can provide substantial capability at a lower acquisition cost. Its broad rotor ecosystem, 120V operation, compact footprint, and ability to process common laboratory vessels make it particularly useful as a general-purpose centrifuge.

CLICK HERE to see the Eppendorf 5810 Benchtop Centrifuge on our YouTube channel