Baker SCI-tive Hypoxia Workstation
Shipping
All products ship from our 30,000 sq. ft. Haverhill, MA warehouse
Shipping
All products ship from our 30,000 sq. ft. Haverhill, MA warehouse
The Baker SCI-tive Hypoxia Workstation is an advanced controlled-atmosphere cell culture system designed to let researchers culture, manipulate, observe, and analyze cells without repeatedly exposing them to ambient laboratory oxygen. Often described as a hypoxia workstation, physiological oxygen workstation, or cell culture glovebox, the SCI-tive provides precise control of oxygen, carbon dioxide, temperature, and humidity inside a large enclosed workspace.
Unlike a conventional CO₂ incubator, which maintains culture conditions only while samples remain behind a closed door, the SCI-tive creates a complete working environment in which cell culture procedures can be performed under the same controlled atmosphere used for incubation. Researchers can seed cultures, feed cells, perform manipulations, conduct assays, use compatible analytical equipment, and harvest samples without routinely transferring cultures to an open laboratory bench.
This capability is especially important for hypoxia and physiological oxygen research. Cells cultured at reduced oxygen concentrations can respond rapidly when exposed to normal atmospheric oxygen. By maintaining continuous environmental control throughout the workflow, the Baker SCI-tive allows researchers to investigate cell behavior under conditions designed to more closely reproduce physiologically relevant oxygen environments.
With approximately 420 liters of usable chamber volume, the SCI-tive provides enough internal space for cell culture vessels, racks, microscopes, imaging systems, analyzers, and other compatible equipment. Glove-port access allows researchers to perform procedures inside the chamber, while a controlled interlock provides a means of transferring materials into and out of the workstation with reduced disruption to the established atmosphere.
This Baker SCI-tive Hypoxia Workstation is used and refurbished by American Instrument Exchange. AIE technicians inspect and test the system before shipment, including environmental controls, chamber integrity, access systems, alarms, controls, and other installed functions. The workstation is cleaned and includes a 90-day warranty.
Advanced physiological oxygen and hypoxia cell culture workstation
Approximately 420 L usable chamber volume
Controlled oxygen environment for hypoxia, physoxia, and related cell culture research
Oxygen control approximately 0.1% to 23%
Carbon dioxide control approximately 0.1% to 30%
Temperature control from approximately ambient +5°C to 45°C
Humidity control up to approximately 85% RH
Continuous controlled atmosphere during culture and cell manipulation
Direct glove-port access to the internal workspace
Controlled transfer interlock
Spacious interior accommodates compatible analytical and imaging equipment
Internal electrical connections for compatible equipment
Optional HEPA filtration depending on installed configuration
Used and refurbished by American Instrument Exchange
90-day warranty included
The Baker SCI-tive is more than an incubator and more specialized than a general-purpose laboratory glovebox.
It creates an enclosed cell culture workspace in which oxygen, carbon dioxide, temperature, and humidity can be maintained at user-selected conditions while researchers actively work with their cultures.
This addresses a significant challenge in hypoxia and physiological oxygen research.
A conventional controlled-oxygen incubator can maintain reduced oxygen while culture vessels are inside the chamber. Once those vessels are removed for feeding, microscopy, manipulation, sampling, or assay preparation, however, they are exposed to ambient laboratory conditions.
Atmospheric oxygen is approximately 21%, substantially higher than the oxygen concentrations encountered by many cells within living tissues.
The SCI-tive allows much of the workflow to remain inside the controlled environment, reducing the need for repeated transitions between experimental and ambient conditions.
The Baker SCI-tive is well suited to hypoxia research, physiological oxygen cell culture, cancer biology, tumor microenvironment studies, stem cell research, regenerative medicine, cell and gene therapy development, drug discovery, translational research, metabolic research, developmental biology, primary cell culture, organoid research, tissue engineering, ischemia research, and other experiments in which oxygen concentration can influence cellular behavior.
The large internal chamber also allows compatible analytical equipment to be operated inside the controlled atmosphere, enabling researchers to extend physiological oxygen conditions beyond incubation alone.
The SCI-tive is particularly valuable for cancer research laboratories, stem cell laboratories, cell and gene therapy developers, biotechnology companies, pharmaceutical R&D groups, academic research institutions, regenerative medicine programs, translational research laboratories, tissue engineering groups, drug-discovery laboratories, and other facilities studying oxygen-sensitive biological processes.
It can be especially valuable for laboratories where researchers have outgrown the limitations of a conventional tri-gas incubator and need to manipulate cultures while maintaining the experimental oxygen environment.
| Specification | Details |
|---|---|
| Manufacturer | The Baker Company / Baker Ruskinn |
| Product Family | SCI-tive |
| Equipment Type | Hypoxia / Physiological Oxygen Cell Culture Workstation |
| Usable Chamber Volume | Approx. 420 L |
| Oxygen Control | Approx. 0.1%–23% |
| Carbon Dioxide Control | Approx. 0.1%–30% |
| Temperature Range | Approx. Ambient +5°C to 45°C |
| Humidity | Up to Approx. 85% RH |
| Chamber Access | Glove Ports |
| Material Transfer | Controlled Interlock |
| Internal Power | Multiple Internal Power Connections |
| Vacuum Connection | Available |
| Gas Sample Port | Available |
| Cable Access | Multi-Cable Gland |
| Culture Capacity | Up to Approx. 180 T75 Flasks |
| HEPA Filtration | Configuration Dependent / Optional |
| Enhanced User Containment | Configuration Dependent |
| Condition | Used / Refurbished |
| Warranty | 90 Days from American Instrument Exchange |
The defining advantage of the SCI-tive is the ability to maintain cells under controlled oxygen conditions throughout much more of the experimental workflow.
A standard incubator maintains the selected environment while its door remains closed. Researchers must normally remove cultures for media changes, passaging, microscopy, harvesting, or other manipulations.
The SCI-tive turns the controlled environment into a workspace.
Researchers can reach through the glove ports and perform procedures inside the chamber while the workstation continues regulating the atmosphere surrounding the cells.
For oxygen-sensitive experiments, this can substantially reduce unwanted environmental changes between incubation and manipulation.
Hypoxia plays an important role in many biological processes.
Reduced oxygen conditions can influence cell signaling, metabolism, gene expression, differentiation, proliferation, angiogenesis, stress responses, and other cellular functions.
The SCI-tive allows researchers to establish controlled low-oxygen environments and maintain those conditions while cultures are actively handled.
This makes the workstation particularly useful when brief exposure to atmospheric oxygen could become an uncontrolled experimental variable.
Not all reduced-oxygen research involves extreme hypoxia.
Many mammalian tissues naturally experience oxygen concentrations considerably below atmospheric levels. Researchers increasingly use physiologically relevant oxygen conditions, sometimes referred to as physoxia, to model these environments more accurately in vitro.
The SCI-tive can provide a controlled environment for these experiments while allowing cells to remain at the selected oxygen concentration during routine manipulation.
This makes it useful for researchers studying the relationship between in vitro culture conditions and in vivo cell behavior.
The SCI-tive platform provides controlled oxygen conditions across a broad range, allowing laboratories to establish environments appropriate for different experimental models.
Nitrogen is typically used to reduce oxygen concentration below atmospheric levels.
The ability to define and maintain oxygen conditions allows one workstation to support research ranging from pronounced hypoxia to moderate physiological oxygen and near-atmospheric conditions.
Exact gas requirements and operating configuration should be confirmed for the individual system before installation.
Carbon dioxide control allows the SCI-tive to support common bicarbonate-buffered mammalian cell culture media.
Maintaining the appropriate CO₂ concentration helps regulate media pH while cells remain inside the workstation.
Because CO₂ control extends throughout the working chamber, researchers can perform cell culture manipulations without immediately removing cultures from their controlled CO₂ environment.
This contributes to the SCI-tive's ability to function as an integrated cell culture workspace rather than simply an incubation chamber.
The SCI-tive maintains a heated working environment suitable for cell culture, with platform specifications extending to approximately 45°C.
Temperature control throughout the chamber allows cultures to remain within the controlled environment during both incubation and active manipulation.
This can reduce the temperature fluctuations that occur when culture vessels are repeatedly moved between an incubator and an ambient laboratory bench.
Humidity control helps limit evaporation during cell culture and contributes to environmental stability within the workstation.
The SCI-tive platform can maintain high relative humidity appropriate for extended cell culture.
This is particularly useful when cultures remain inside the workstation for extended periods or when researchers are working with small liquid volumes that can be sensitive to evaporation.
Humidity capability and operating limits should be confirmed for the exact workstation configuration and experimental conditions.
The SCI-tive provides approximately 420 liters of usable internal chamber volume.
This gives researchers substantially more working space than a conventional small glovebox or incubator.
The chamber can accommodate cell culture vessels, racks, pipettes, media containers, microscopes, imaging equipment, and other compatible instruments required during an experiment.
Baker indicates capacity of up to approximately 180 T75 flasks under appropriate configurations.
Actual working capacity depends on culture-rack arrangement, installed instruments, and the amount of open workspace required for manipulation.
The large chamber allows the SCI-tive to function as a self-contained cell culture laboratory.
Rather than using the workstation only for incubation, researchers can place compatible equipment inside the chamber and perform multiple experimental steps without returning cultures to an open bench.
This can include media changes, passaging, visual inspection, imaging, sampling, assay preparation, and harvesting.
For experiments where oxygen exposure matters, consolidating these steps within one controlled environment can simplify workflow design and reduce environmental transitions.
Integrated glove ports allow researchers to manipulate cultures and equipment without opening the main chamber.
The operator can reach into the workstation while remaining physically separated from the internal environment.
This provides the dexterity required for common cell culture procedures while allowing the workstation to maintain its controlled atmosphere.
Glove and sleeve components should be inspected routinely for damage and replaced when necessary to maintain chamber integrity.
Materials must frequently enter and leave the workstation during an experiment.
The SCI-tive incorporates a transfer interlock so culture vessels, media, consumables, and other materials can be introduced without opening the entire working chamber to room air.
The interlock is designed to minimize atmospheric disruption during transfers.
Proper use of the interlock is important when maintaining low oxygen concentrations because opening the main chamber directly to the laboratory would rapidly alter the internal environment.
The transfer interlock can maintain controlled conditions during the transfer process, helping reduce the environmental transition experienced by cultures and materials entering the main chamber.
This is especially useful for oxygen-sensitive workflows in which abrupt exposure to ambient oxygen could affect experimental conditions.
Transfer protocols should be established according to the oxygen level, materials involved, and requirements of the laboratory's experimental method.
The SCI-tive provides internal electrical connections that allow compatible laboratory equipment to operate inside the controlled atmosphere.
This capability significantly expands the types of procedures that can be performed without removing cells from the workstation.
Researchers may be able to install compatible microscopes, imaging systems, mixers, small analyzers, or other instrumentation depending on physical size, power requirements, heat generation, and compatibility with the workstation atmosphere.
Equipment suitability should be evaluated before installation inside the chamber.
A multi-cable gland allows connections to pass between internal equipment and external systems.
This is useful for analytical instruments, imaging systems, sensors, data acquisition hardware, and other equipment requiring communication outside the chamber.
The ability to integrate instrumentation is one of the major differences between a large physiological oxygen workstation and a conventional controlled-oxygen incubator.
Tumor biology is one of the most important applications for controlled-oxygen workstations.
Solid tumors frequently contain regions where oxygen availability differs substantially from atmospheric culture conditions.
Researchers studying tumor metabolism, therapeutic response, angiogenesis, cell signaling, invasion, and other processes may therefore need to culture and manipulate cells at reduced oxygen concentrations.
The SCI-tive allows these workflows to remain within a controlled environment from culture through experimental manipulation.
Stem cells can respond strongly to environmental conditions, including oxygen concentration.
Physiological oxygen culture is therefore important in many stem cell and regenerative medicine studies.
The SCI-tive allows researchers to establish controlled oxygen conditions while reducing repeated transitions between an incubator and ambient air.
This can be useful when studying stem cell maintenance, expansion, differentiation, metabolism, and other oxygen-sensitive processes.
Cell and gene therapy workflows often involve sensitive living cell populations that undergo multiple culture and manipulation steps.
A controlled-atmosphere workstation can allow researchers to perform these procedures while maintaining selected oxygen, CO₂, temperature, and humidity conditions.
The SCI-tive can therefore support research and process-development workflows where environmental consistency is important.
Use in regulated manufacturing environments requires facility-specific qualification and validation and should not be assumed solely from the workstation's capabilities.
Organoids, spheroids, and other three-dimensional cell models are increasingly used to reproduce aspects of tissue physiology in vitro.
Oxygen gradients can play an important role in these models.
A physiological oxygen workstation allows researchers to manipulate cultures without repeatedly returning them to atmospheric oxygen.
This can be particularly valuable when investigating tumor spheroids, stem-cell-derived organoids, tissue models, and other complex culture systems.
Drug response observed under atmospheric oxygen does not always reproduce behavior under physiologically relevant conditions.
The SCI-tive allows researchers to investigate candidate compounds and cellular responses under defined oxygen environments.
This capability can support translational studies seeking to create experimental conditions that better represent the biological environment being modeled.
The workstation itself does not guarantee improved biological relevance; appropriate experimental design and validation remain essential.
A conventional CO₂ incubator provides a stable temperature, humidity, and CO₂ environment while cultures are stored inside.
Some incubators also provide oxygen control.
The principal limitation is that cultures generally leave that environment whenever researchers need to manipulate them.
The SCI-tive extends environmental control into the actual working space.
Researchers can incubate and manipulate cultures under the selected atmosphere, making it particularly useful when oxygen exposure during handling is experimentally important.
A tri-gas incubator can maintain reduced oxygen during incubation by controlling oxygen, carbon dioxide, and nitrogen.
For many applications, that is sufficient.
The SCI-tive becomes especially valuable when researchers need to perform substantial manipulation under the same oxygen conditions.
Rather than transferring vessels from a tri-gas incubator to an open bench, researchers can work directly inside the controlled chamber.
The appropriate choice depends on whether continuous environmental control during manipulation is important to the experimental design.
Traditional laboratory gloveboxes are commonly designed to provide an inert, dry, oxygen-free, or otherwise controlled atmosphere for chemical and materials applications.
The SCI-tive is specifically optimized around biological cell culture.
Its combination of oxygen, CO₂, temperature, and humidity control supports living cell cultures rather than simply creating an inert atmosphere.
It should therefore be considered a physiological cell culture workstation rather than a generic inert-atmosphere glovebox.
The Baker SCI-tive should not automatically be treated as a replacement for a Class II biosafety cabinet.
A biosafety cabinet is specifically designed and tested to provide personnel and environmental protection from appropriate biological aerosols while also providing product protection.
The standard SCI-tive is primarily designed to create and maintain a controlled cell culture environment.
Baker offers configuration-dependent HEPA filtration and enhanced containment options, but user protection capabilities depend on the specific workstation configuration.
Laboratories handling hazardous biological materials should perform an appropriate risk assessment and use containment equipment suitable for the required biosafety level and procedure.
Baker offers HEPA filtration for the SCI-tive platform to provide cleaner air within the working environment and help protect cultures from airborne contamination.
Importantly, HEPA filtration is configuration dependent and should not be assumed to be installed on every SCI-tive workstation.
Customers considering this used system should confirm whether HEPA filtration is present on the specific unit being purchased.
HEPA filtration also should not automatically be interpreted as providing biosafety-cabinet-level personnel containment.
Maintaining a closed cell culture environment can reduce the frequency with which cultures are exposed to the surrounding laboratory.
This can support contamination-control practices by keeping cell culture workflows within a dedicated enclosed space.
Good aseptic technique remains essential.
Researchers should establish appropriate procedures for transferring materials, cleaning interior surfaces, maintaining gloves and sleeves, managing humidity, and introducing equipment into the chamber.
Baker offers an enhanced containment package for SCI-tive systems where additional operator-protection capability is required.
Because this is configuration dependent, enhanced containment should not be assumed on a used SCI-tive unless the installed components and configuration have been verified.
Customers who require personnel containment for a specific biological application should discuss those requirements with American Instrument Exchange before purchase.
Controlled oxygen and carbon dioxide environments require appropriate laboratory gas supplies.
Depending on the operating configuration, the workstation may use nitrogen to reduce oxygen concentration along with carbon dioxide for cell culture control.
Gas quality, pressure, regulators, tubing, and facility connections should meet Baker's requirements for the specific system.
Customers should review utility requirements before delivery so the workstation can be installed and tested efficiently.
A physiological oxygen workstation is most valuable when maintaining controlled oxygen during active cell manipulation is important to the experiment.
Laboratories that only need reduced oxygen during incubation may find a tri-gas incubator sufficient.
The SCI-tive becomes particularly compelling when cultures must be fed, passaged, imaged, analyzed, or otherwise manipulated without repeated exposure to atmospheric oxygen.
The large chamber also makes it appropriate for laboratories that want to integrate compatible analytical equipment directly into the controlled environment.
American Instrument Exchange inspects and tests used Baker SCI-tive workstations before shipment.
Testing includes inspection of the chamber, glove and sleeve access points, seals, transfer interlock, gas connections, controls, displays, alarms, environmental-control components, and other installed systems.
Chamber integrity is evaluated for leaks, and AIE performs pressure-decay testing to verify that the workstation maintains pressure appropriately.
Environmental controls are operated and tested to confirm proper response and stability. Installed accessories and configuration-dependent features are also evaluated as applicable.
Accessible interior and exterior surfaces are thoroughly cleaned before shipment.
If your laboratory has particular testing requirements, American Instrument Exchange can discuss those requirements before the system ships.
Physiological oxygen workstations are sophisticated cell culture systems, and purchasing refurbished equipment can significantly reduce the cost of adding continuous hypoxia or physoxia capability to a laboratory.
The Baker SCI-tive provides a large controlled workspace where researchers can maintain oxygen, carbon dioxide, temperature, and humidity while actively manipulating cell cultures.
For cancer research, stem cell biology, regenerative medicine, cell and gene therapy, organoid research, drug discovery, and other oxygen-sensitive applications, this capability can provide experimental flexibility that conventional incubators cannot offer.
American Instrument Exchange tests and cleans this used SCI-tive before shipment and includes a 90-day warranty.
The Baker SCI-tive is a large controlled-atmosphere workstation that requires appropriate planning before installation.
Customers should confirm laboratory space, access dimensions, electrical service, gas supplies, regulators, ventilation considerations, and clearance requirements before delivery.
The final installation should provide adequate space for chamber access, gas connections, interlock operation, maintenance, and any analytical equipment that will be installed inside the workstation.
After installation and connection to the required utilities, the chamber and environmental controls should be evaluated before experimental cultures are introduced.
Laboratories using a Baker SCI-tive Hypoxia Workstation may also require CO₂ incubators, tri-gas incubators, biosafety cabinets, inverted microscopes, centrifuges, laboratory refrigerators, laboratory freezers, ultra-low freezers, cryogenic storage systems, water baths, shakers, and other equipment supporting mammalian cell culture and translational research.
American Instrument Exchange supplies used and refurbished cell culture equipment for biotechnology, pharmaceutical, cell and gene therapy, academic, clinical, and research laboratories.
The Baker SCI-tive is a physiological oxygen cell culture workstation that provides a controlled environment for culturing and manipulating cells under user-selected oxygen, CO₂, temperature, and humidity conditions.
Yes. Hypoxia and physiological oxygen cell culture are primary applications of the SCI-tive platform.
The SCI-tive platform supports controlled oxygen conditions from approximately 0.1% to 23%, depending on configuration and operating conditions.
Yes. It is specifically designed to support cell culture under controlled physiological oxygen conditions as well as more pronounced hypoxia.
Yes. The platform provides CO₂ control for cell culture applications, with published capabilities extending approximately from 0.1% to 30%.
Yes. The workstation maintains a heated environment, with published platform specifications extending from approximately ambient +5°C to 45°C.
Yes. The SCI-tive platform can maintain elevated humidity, with published capability up to approximately 85% relative humidity.
The standard SCI-tive provides approximately 420 liters of usable chamber volume.
Baker indicates capacity of up to approximately 180 T75 flasks, although practical capacity depends on rack arrangement, installed equipment, and required working space.
Yes. This is one of the SCI-tive's principal advantages. Glove-port access allows researchers to manipulate cultures while maintaining the controlled internal atmosphere.
Yes. Its large interior and internal electrical connections allow compatible microscopes, imaging systems, analyzers, and other instruments to operate within the controlled environment.
A controlled transfer interlock allows materials to enter and leave the workstation while minimizing disruption to the main chamber atmosphere.
A tri-gas incubator maintains controlled oxygen while cultures remain inside it. The SCI-tive additionally allows researchers to manipulate cultures within the same controlled atmosphere.
Yes. Tumor hypoxia and physiological oxygen studies are important applications for the SCI-tive.
Yes. Baker specifically positions SCI-tive for physiological oxygen cell culture, including stem cell research where oxygen conditions may influence cellular behavior.
It can be valuable for organoid and 3D cell culture research when maintaining defined oxygen conditions during manipulation is important to the experimental design.
Yes. The SCI-tive can support cell and gene therapy research and process-development workflows requiring controlled cell culture environments. Use in regulated manufacturing requires appropriate facility qualification and validation.
No. HEPA filtration is configuration dependent. Customers should verify whether it is installed on the specific used workstation being purchased.
When installed, HEPA filtration helps provide cleaner air within the working environment to protect cultures from airborne particulate contamination.
No. HEPA filtration alone does not make a workstation equivalent to a Class II biosafety cabinet.
The standard platform is primarily intended to provide a controlled environment for cell culture. Baker offers an enhanced containment package for applications requiring additional user protection. The exact configuration should be verified before relying on the workstation for personnel containment.
It is primarily a physiological oxygen and hypoxia cell culture workstation. Laboratories requiring strict anaerobic conditions should verify whether the specific model and configuration meet their required oxygen limits and application.
Typical controlled-oxygen cell culture operation requires appropriate gas supplies such as nitrogen and carbon dioxide. Exact gas and utility requirements should be confirmed for the individual workstation.
Yes. Media changes and other manipulations can be performed through the glove ports while the culture remains inside the controlled chamber.
Compatible microscopy or imaging equipment can be placed inside the workstation, allowing appropriate observations to occur without routinely exposing cultures to ambient oxygen.
The large chamber provides room not only for culture vessels but also for racks, supplies, and compatible analytical or imaging equipment, allowing more of the experimental workflow to occur inside the controlled atmosphere.
This workstation is used and refurbished by American Instrument Exchange.
AIE inspects and tests the chamber, environmental controls, gas connections, glove access, transfer system, controls, alarms, seals, and installed accessories. Chamber integrity is also evaluated, including pressure-decay testing.
Yes. American Instrument Exchange includes a 90-day warranty with this refurbished SCI-tive Hypoxia Workstation.
Yes, all equipment from American Instrument Exchange comes with a warranty.
Used/reconditioned/refurbished equipment is tested to make sure it operates to the manufacturer's specifications and comes with a 90-day warranty (unless otherwise noted). New equipment comes with a warranty from the manufacturer.
Testing requirements vary across all the equipment categories we sell. Temperature controlled units are tested with NIST traceable probes and data loggers. Gaskets, external housing, glass, handles, controls and control screens are all thoroughly inspected for integrity and functionality.
We test all equipment using the manufacturer's parameters and specifications to confirm that the unit operates properly.
We can ship products both nationally and internationally using a well-established network of transporters. Smaller items will ship via UPS. Larger equipment will be palletized and shipped either LTL or air-ride, depending on the specific equipment and/or delivery requirements.
American Instrument Exchange will accept the return of any product that is not working as represented and could not be replaced or repaired to the customer's satisfaction during the warranty period. Customers who cancel orders after refurbishing has begun will be subject to a cancellation and restocking fee of between 20% and 40%.
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