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Eppendorf 5417R Refrigerated Microcentrifuge with 30-Place Rotor
The Eppendorf 5417R Refrigerated Microcentrifuge has long been a dependable instrument in molecular biology, biotechnology, and life science laboratories. Combining high-speed microcentrifugation with precise refrigeration, this compact benchtop centrifuge is designed for routine DNA and RNA purification, protein processing, sample clarification, and other temperature-sensitive laboratory procedures.
This particular Eppendorf 5417R from American Instrument Exchange includes a 30-place rotor for 1.5/2.0 mL microcentrifuge tubes, providing useful sample capacity for laboratories performing repetitive extraction, purification, and preparation protocols. The centrifuge platform delivers maximum performance of 16,400 RPM and 25,000 × g with an appropriate rotor, while its refrigeration system provides adjustable temperatures from -9°C to +40°C.
Although the 5417R has been succeeded by newer Eppendorf microcentrifuges, its straightforward controls, compact design, and established performance continue to make it a practical option for laboratories seeking a reliable refrigerated microcentrifuge at a lower acquisition cost.
American Instrument Exchange professionally inspects, reconditions, and tests its refurbished centrifuges before shipment. Each unit includes a 90-day warranty.
Key Features of the Eppendorf 5417R
Includes a 30-place rotor for 1.5/2.0 mL microcentrifuge tubes
Maximum centrifuge platform speed of 16,400 RPM
Maximum platform RCF of 25,000 × g, rotor dependent
Standard F-45-30-11 rotor rated to 14,000 RPM and 20,800 × g
Refrigerated temperature range from -9°C to +40°C
Fast Cool function for rapid pre-cooling
Automatic rotor recognition and imbalance detection
SOFT function for gentler acceleration and braking
Short-spin function for quick sample collection
Selectable RPM or RCF display
Timer up to 99 minutes or continuous operation
Compact, low-profile benchtop construction
120V electrical configuration
Refurbished and tested by American Instrument Exchange
90-day warranty included
A Proven Refrigerated Microcentrifuge for Everyday Laboratory Work
The 5417R was designed for laboratories requiring reliable, high-speed centrifugation of small biological samples. Its combination of refrigeration, programmable operating parameters, and multiple rotor configurations made it a popular choice in molecular biology laboratories and research facilities.
Unlike larger general-purpose centrifuges designed for conical tubes and bottles, the 5417R focuses on small-volume processing. This specialization makes it especially useful for laboratories performing repeated centrifugation steps during nucleic acid extraction, sample preparation, and protein purification.
The included 30-place rotor offers greater sample capacity than conventional 24-place microcentrifuge configurations. For laboratories routinely processing 25 to 30 samples at a time, this can reduce the number of centrifugation cycles required.
The low-profile design also provides convenient access to the rotor chamber, while the straightforward keypad controls allow technicians to adjust speed, centrifugal force, temperature, and run time without navigating complex software menus.
Common Applications
The Eppendorf 5417R is well suited for molecular biology and biotechnology workflows involving small-volume samples.
Typical applications include DNA and RNA extraction, plasmid purification, spin-column processing, nucleic acid precipitation, protein precipitation, cell lysate clarification, enzyme preparation, PCR sample processing, microbiology research, and general laboratory sample preparation.
The refrigerated design is particularly useful when protocols specify controlled temperatures during centrifugation. This can be important for temperature-sensitive proteins, enzymes, nucleic acids, and other biological materials.
Included 30-Place Rotor and Rotor Compatibility
This Eppendorf 5417R includes a rotor accommodating 30 standard 1.5/2.0 mL microcentrifuge tubes.
Eppendorf offered several rotor configurations for the 5417 series, allowing laboratories to select the capacity and centrifugal force best suited to their applications.
The standard F-45-30-11 fixed-angle rotor accommodates 30 microcentrifuge tubes and operates at up to 14,000 RPM and 20,800 × g. An aerosol-tight FA-45-30-11 version provides similar capacity and performance when properly fitted with its sealing lid.
Other compatible configurations historically included a 24-place high-speed fixed-angle rotor capable of reaching the platform maximum of 16,400 RPM and 25,000 × g, an eight-place swinging-bucket rotor, and a rotor designed for PCR tubes and strips.
The rotor supplied with this particular unit provides 30-place microtube capacity. Customers should confirm its exact rotor model, lid, adapters, and accessories before purchase, as rotor configurations and included components can vary.
Refrigeration and Temperature Control
The Eppendorf 5417R provides adjustable temperature settings from -9°C to +40°C, allowing laboratories to perform centrifugation procedures under controlled thermal conditions.
Its Fast Cool function can bring the centrifuge chamber from approximately 20°C to 4°C in roughly 20 minutes under specified operating conditions. This allows technicians to prepare the instrument before loading temperature-sensitive samples.
The centrifuge also provides standby cooling to help maintain the chamber temperature between runs.
These features are useful in laboratories performing repeated sample-preparation procedures throughout the day, particularly when maintaining consistent temperature conditions is important for reproducibility.
Actual sample temperature depends on rotor selection, operating speed, sample load, and ambient conditions.
Eppendorf 5417R Key Specifications
Specification
Details
Manufacturer
Eppendorf
Model
5417R
Condition
Used / Refurbished
Centrifuge Type
Refrigerated Microcentrifuge
Included Rotor
30-Place Microtube Rotor
Included Rotor Capacity
30 × 1.5/2.0 mL
Standard 30-Place Rotor Maximum Speed
14,000 RPM
Standard 30-Place Rotor Maximum RCF
20,800 × g
Maximum Platform Speed
16,400 RPM, rotor dependent
Maximum Platform RCF
25,000 × g, rotor dependent
Temperature Range
-9°C to +40°C
Fast Cool Function
Yes
Rotor Recognition
Automatic
Imbalance Detection
Automatic
SOFT Acceleration / Braking
Yes
Short-Spin Function
Yes
Timer
1–99 Minutes / Continuous
Dimensions
12.2"W × 23.5"D × 9.8"H
Weight
Approximately 77 lb
Electrical Requirements
120V, 50/60 Hz
Maximum Power Requirement
Approximately 700 W
Warranty
90 Days
Specifications compiled from Eppendorf manufacturer documentation, distributor technical references, and American Instrument Exchange product information. Rotor-specific specifications should be verified against the rotor supplied with the instrument.
Power Requirements and Installation
The Eppendorf 5417R operates on 120V electrical service, making it suitable for installation in many U.S. laboratory environments.
Measuring approximately 12.2 inches wide, 23.5 inches deep, and 9.8 inches high, the centrifuge provides high-speed refrigerated operation without requiring the bench space associated with larger multipurpose centrifuges.
At approximately 77 pounds, it should be positioned on a sturdy, level laboratory bench with adequate clearance for ventilation and access to the rotor chamber.
The refrigeration system requires unobstructed airflow around the instrument. Laboratories should follow the manufacturer's installation and ventilation recommendations to maintain proper cooling performance.
Is the Eppendorf 5417R Still a Good Laboratory Centrifuge?
The 5417R remains a practical option for laboratories that need refrigerated microcentrifugation but do not necessarily require the additional features of a current-generation instrument.
Its principal advantages are its established design, relatively straightforward operation, 30-place sample capacity, and ability to perform routine high-speed molecular biology procedures.
However, the 5417R is a discontinued model, and replacement parts and certain rotor accessories may be more difficult to obtain than those for newer Eppendorf centrifuges.
For laboratories evaluating an existing 5417R, the condition of the refrigeration system, motor, bearings, electronic controls, and rotor should be considered when determining whether continued service is practical.
Laboratories looking for a newer alternative may consider the Eppendorf 5424R, 5425R, or 5430R, depending on required capacity, rotor flexibility, and maximum centrifugal force.
A properly tested refurbished 5417R can still provide considerable value for laboratories whose applications fall within its operating capabilities.
Refurbishment and Testing at American Instrument Exchange
American Instrument Exchange technicians inspect and test refurbished centrifuges before shipment to verify proper mechanical and electrical operation.
Testing includes evaluation of the rotating assembly, spindle and bearing condition, seals and gaskets, braking, electronic controls, lid safety mechanisms, and rotor recognition.
Because the Eppendorf 5417R is refrigerated, technicians also evaluate cooling performance to confirm that the instrument reaches and maintains appropriate operating temperatures.
When a rotor is supplied with a centrifuge, the rotor and instrument are tested together to evaluate operation as a complete system.
The centrifuge is thoroughly cleaned and prepared for shipment, and the refurbished unit is backed by American Instrument Exchange's 90-day warranty.
Related Laboratory Equipment
American Instrument Exchange offers a broad selection of used and refurbished laboratory centrifuges, including refrigerated microcentrifuges, general-purpose benchtop centrifuges, and high-speed floor-model systems.
American Instrument Exchange and AIE Laboratory Services provide diagnostic evaluation, preventative maintenance, and repair services for centrifuges and other laboratory equipment.
For an older refrigerated microcentrifuge such as the 5417R, service evaluation may include the drive motor, bearings, refrigeration components, temperature sensors, lid interlocks, electronic controls, and rotor condition.
Because replacement parts for discontinued centrifuges may be limited, our technicians can help laboratories determine whether repair or replacement with another refurbished instrument is the more economical option.
Eppendorf 5417R Frequently Asked Questions
Does this Eppendorf 5417R include a rotor?
Yes. This particular unit includes a 30-place rotor designed for 1.5/2.0 mL microcentrifuge tubes. Customers should confirm the exact rotor model and included accessories before purchase.
What is the maximum speed of the Eppendorf 5417R?
The centrifuge platform can reach 16,400 RPM and 25,000 × g with an appropriate high-speed rotor. The standard 30-place F-45-30-11 rotor is rated for 14,000 RPM and 20,800 × g.
What is the difference between the Eppendorf 5417C and 5417R?
The primary difference is refrigeration. The 5417R provides adjustable temperature control from -9°C to +40°C, while the 5417C is a non-refrigerated model. Both share similar high-speed microcentrifuge capabilities.
Can the Eppendorf 5417R maintain samples at 4°C?
The 5417R is designed for refrigerated centrifugation and can maintain low chamber temperatures during operation. Eppendorf documents the ability to maintain sample temperatures below 4°C at maximum speed under specified conditions. Actual performance depends on rotor selection, loading, and operating conditions.
Can the Eppendorf 5417R process PCR tubes?
Yes. Compatible rotor configurations and adapters were available for PCR tubes and strips. However, this particular unit includes a 30-place microtube rotor, so customers should verify the required adapters or alternative rotor before purchase.
Is the Eppendorf 5417R suitable for DNA and RNA purification?
Yes. DNA and RNA extraction, precipitation, and spin-column purification are among the common applications for the 5417R. Its refrigeration system is useful when protocols require temperature-controlled sample processing.
What does the SOFT function do?
The SOFT function provides gentler acceleration and deceleration. This can help minimize disturbance of sensitive samples or pellets during centrifugation.
How does the Eppendorf 5417R compare with the 5424R or 5425R?
The 5417R is an older refrigerated microcentrifuge with a 30-place rotor option. Newer models such as the 5424R and 5425R offer updated controls, rotor systems, and other design improvements. The best choice depends on required sample capacity, rotor compatibility, budget, and the importance of ongoing manufacturer support.
Are replacement parts still available for the 5417R?
Availability varies by component. Because the 5417R is discontinued, certain parts and accessories may be difficult to obtain. American Instrument Exchange can help evaluate repair feasibility and potential replacement options.
Why purchase a refurbished Eppendorf 5417R?
For laboratories that need reliable refrigerated microcentrifugation without the cost of a new instrument, a professionally tested 5417R can be a practical solution. Its 30-place capacity, high-speed performance, refrigeration, and straightforward operation remain useful for many routine molecular biology applications.
Click Hereto see the Eppendorf 5417R Microfuge on the American Instrument Exchange YouTube channel.
Testing Procedures for Used and Refurbished Laboratory Centrifuges
Ensure Quality, Accuracy, and Compliance Before Deployment
Used and refurbished laboratory centrifuges from American Instrument Exchange undergo testing procedures to ensure they meet the performance, safety, and compliance standards required in modern laboratories.
Why Testing Used Laboratory Centrifuges Is Essential
Before a centrifuge is reintroduced into a lab environment, it must be tested for:
Mechanical integrity
Rotor balance and speed accuracy
Safety system functionality
Electrical compliance
Proper testing helps extend equipment lifespan, reduce downtime, and ensure safety in high-stakes lab environments such as clinical diagnostics, biotechnology, and research applications.
Our structured testing protocol for used and refurbished centrifuges ensures equipment safety, accuracy, and longevity.
Key Testing Procedures for Refurbished and Used Centrifuges
1. Initial Visual Inspection
Check for signs of wear, corrosion, or damage to housing, lid, and control panels
Inspect power cords, plugs, and connectors for fraying or exposed wiring
Confirm that the rotor and accessories are compatible and undamaged
2. Functional Testing
Power up the centrifuge and confirm successful boot-up of digital or analog displays
Test lid lock and emergency stop mechanisms
Run initial cycles at various speeds to check for abnormal noise or vibration
3. Rotor and Speed Verification
Test all programmable speed settings to confirm consistent acceleration and deceleration
Monitor temperature, vibration, and RPM consistency throughout the cycle
Balance testing ensures smooth operation and prevents wear on internal components
4. Electrical Safety and Grounding Tests
Verify that all electrical components are properly grounded and shielded
5. Temperature Control (if applicable)
For refrigerated centrifuges, test cooling function to ensure temperature stability within set ranges
Check refrigerant levels and inspect for compressor noise or failure
6. Final Quality Assurance Checklist
Conduct a full run-through using the user interface
Document all test results
Repair and Refurbishment Protocol for Used Laboratory Centrifuges
Certified Quality from American Instrument Exchange
At American Instrument Exchange, we follow a meticulous repair and refurbishment protocol to ensure that every used laboratory centrifuge we sell. We strive for reliable performance and long-term durability—providing labs with exceptional value on high-quality pre-owned centrifuges.
Why Choose a Refurbished Centrifuge from American Instrument Exchange?
Fully tested
Backed by warranty and performance guarantee
Cost-effective alternative to new lab equipment
Environmentally responsible through equipment reuse
Supported by expert technicians with decades of experience
Step-by-Step Refurbishment Protocol
Every used centrifuge at American Instrument Exchange goes through the following comprehensive repair and refurbishment process:
1. Initial Intake & Inspection
Our technicians perform a complete visual and mechanical inspection
Evaluation of hinges, seals, gaskets, and electrical components
Any physical damage, missing parts, or wear is documented for repair
2. Cleaning and decontamination
Full internal and external cleaning using non-abrasive, disinfectant-safe products
Biological or chemical residue is decontaminated/cleaned
3. Mechanical & Electrical Repairs
Damaged or worn parts (e.g., drive motors, lid latches, rotor buckets) are replaced or repaired
Electrical components, sensors, and fuses are tested and replaced as needed
Wiring is inspected for integrity and safety per industry standards
4. Rotor & Accessory Evaluation
All rotors, buckets, and adapters are inspected for cracks, corrosion, and balance
Non-conforming components are replaced with OEM or equivalent alternatives
5. Performance Testing
Rotor speed (RPM), acceleration, and deceleration are verified
Refrigerated centrifuges undergo temperature stability testing across set ranges
6. Safety System Verification
Lid lock and interlock systems are tested for proper function
Emergency stop and imbalance detection systems are verified
Electrical safety and grounding tests are conducted
Trust the Experts at American Instrument Exchange
With over 50 years of experience in the industry, American Instrument Exchange is a trusted name for refurbished lab equipment. Our centrifuge refurbishment protocol reflects our commitment to quality, safety, and customer satisfaction.
Warranty Information
All used equipment sold by American Instrument Exchange is refurbished/reconditioned, tested to meet specifications, cleaned, and comes with a 90-day warranty from the date of shipment unless otherwise specified. New products include a warranty from the manufacturer. Your warranty is only valid if you have paid your invoice. If your invoice is over 30 days old, warranty work will not be performed until your invoice is paid in full. American Instrument Exchange is not liable for any special or consequential damages that result from the use of, or the inability to use, the materials on this site or the performance of the products,
Exceptions include international sales and items that are agreed to be sold with a lesser warranty or items that are agreed to be sold “as is.”
Should there be visible shipping damage to packaging or concealed shipping damage to item(s) supplied, buyer must communicate such damage to the delivering entity or carrier upon receipt first and to American Instrument the same day item is accepted / signed for. Failure to do so voids warranty. Customer decision to involve / procure third party or OEM service other than American Instrument or AIE assigned vendors to resolve in-warranty instrument failure or defect without first placing a service call to American Instrument voids warranty.
Return & Cancellation Policy
American Instrument Exchange will authorize the return of and issue a full refund for any equipment that is not working as represented and could not be replaced or repaired to the customer’s satisfaction by AIE or a representative of AIE during the warranty period. Returns without prior authorization and an RMA number will not be accepted.
Customers are responsible for proper packaging of the equipment and all included accessories. American Instrument will pay for insurance and shipping costs. Non-inventory items bought specifically to fill customer orders may possibly be returned, but on a case by case basis.
Customers who cancel orders after refurbishing has begun or after delivery where there are no equipment performance issues may be subject to a Restocking/Cancellation Fee of 20% or more and responsible for shipping costs for both the delivery and return.
Ongoing and Extended Support Options
American Instrument Exchange is able to provide additional repair and support services outside the warranty terms for most products that sell.
For biosafety cabinets, clean benches, chemical fume hoods, and other clean air products our team at AIE Laboratory Services can provide certifications, repairs, decontaminations, and other services for customers throughout New England, and guidance and limited technical assistance for any customers in the US and Canada for new or refurbished biosafety cabinets purchased from American Instrument Exchange.
For all other products, the repair service team at American Instrument Exchange can provide both field and depot repair evaluations. We can easily schedule and coordinate the pick-up and transportation of your malfunctioning equipment. By offering hourly rates lower than most OEM service providers and eliminating travel time for technicians, we help reduce repair costs. Furthermore, our lab depot repair service minimizes or eliminates scheduling delays. Our experienced service technicians are qualified to restore your equipment to factory specifications.
Yes, all equipment from American Instrument Exchange comes with a warranty. Used/reconditioned/refurbished equipment is tested to make sure it operates to the manufacturer's specifications and comes with a 90-day warranty (unless otherwise noted). New equipment comes with a warranty from the manufacturer.
How do you test and recondition the equipment?
Testing requirements vary across all the equipment categories we sell. Temperature controlled units are tested with NIST traceable probes and data loggers. Gaskets, external housing, glass, handles, controls and control screens are all thoroughly inspected for integrity and functionality. We test all equipment using the manufacturer's parameters and specifications to confirm that the unit operates properly.
How do products ship from American Instrument Exchange?
We can ship products both nationally and internationally using a well-established network of transporters. Smaller items will ship via UPS. Larger equipment will be palletized and shipped either LTL or air-ride, depending on the specific equipment and/or delivery requirements.
Return and order cancellation policy
American Instrument Exchange will accept the return of any product that is not working as represented and could not be replaced or repaired to the customer's satisfaction during the warranty period. Customers who cancel orders after refurbishing has begun will be subject to a cancellation and restocking fee of between 20% and 40%.
Are discounts available for educational or non-profit institutions, or for bulk purchases?
Yes – call or email us today with a list of equipment you're looking for and we can potentially put together a quote that includes a discount.
How does your price matching policy work?
We strive to keep our pricing competitive and reasonable. If you find comparable items for sale at a lower price from other vendors please send us a link and or quote for the item and we'll do our best to match or beat the price.
Can I see the equipment in person before committing to a purchase?
Customers are welcome to make an appointment to view equipment in our warehouse. Given our workload we may not be able to test or clean the equipment prior to your arrival if you have not committed to an order.
1023 Western Avenue is a working warehouse, not a showroom, and customers should dress appropriately and wear closed toe shoes.
Can I ship products that I purchased elsewhere to American Instrument for repair?
Yes – please see our repair services page for more information.
