Eppendorf 5430R Refrigerated Centrifuge with S-24-11-AT Rotor

Eppendorf 5430R Refrigerated Centrifuge with S-24-11-AT Swinging-Bucket Rotor

The Eppendorf 5430R Refrigerated Centrifuge with S-24-11-AT Rotor combines high-speed microcentrifugation, precise temperature control, and swinging-bucket rotor technology in a versatile benchtop instrument. Designed for molecular biology, biotechnology, genomics, proteomics, and general laboratory research, the Eppendorf 5430R centrifuge provides reliable performance for laboratories processing temperature-sensitive biological samples.

This particular Eppendorf 5430R is available used and refurbished from American Instrument Exchange and includes the Eppendorf S-24-11-AT aerosol-tight swinging-bucket rotor. The rotor accommodates 24 standard 1.5/2.0 mL microcentrifuge tubes and operates at speeds up to 12,700 RPM, generating centrifugal forces up to 16,049 × g.

Unlike conventional fixed-angle microcentrifuge rotors, the S-24-11-AT allows sample tubes to swing outward into a horizontal position during centrifugation. This design directs sedimentation toward the bottom of the tubes rather than along the outer tube wall, making it useful for procedures requiring consistent pellet positioning and efficient sample recovery.

The refrigerated 5430R platform supports 12 rotor configurations and can reach maximum speeds of 17,500 RPM and centrifugal forces of 30,130 × g with an appropriate high-speed rotor. Its temperature range of -11°C to +40°C helps laboratories maintain controlled conditions for sensitive proteins, enzymes, nucleic acids, and other biological materials.

American Instrument Exchange professionally inspects, cleans, and tests refurbished centrifuges before shipment. This unit includes a 90-day warranty.

Key Features

• Includes Eppendorf S-24-11-AT swinging-bucket rotor

• Aerosol-tight rotor design with QuickLock lid system

• Capacity for 24 × 1.5/2.0 mL microcentrifuge tubes

• Included rotor maximum speed of 12,700 RPM

• Included rotor maximum RCF of 16,049 × g

• Horizontal tube positioning during centrifugation

• Maximum centrifuge platform speed of 17,500 RPM

• Maximum platform RCF of 30,130 × g, rotor dependent

• Refrigerated temperature range from -11°C to +40°C

• FastTemp pre-cooling for temperature-sensitive samples

• Automatic rotor recognition and imbalance detection

• SOFT brake function for sensitive pellets

• Stores up to 50 user-defined programs

• Five direct-access program keys

• Large backlit display

• Compatible with 12 Eppendorf rotor configurations

• Refurbished and tested by American Instrument Exchange

• 90-day warranty included

Overview of the Eppendorf 5430R

The Eppendorf 5430R was developed to bridge the gap between compact microcentrifuges and larger multipurpose laboratory centrifuges.

Traditional microcentrifuges are generally optimized for small-volume samples processed in fixed-angle rotors. Larger general-purpose centrifuges provide greater rotor flexibility but often require substantially more bench space.

The 5430R combines these capabilities in a refrigerated instrument that supports microcentrifuge tubes, conical tubes, PCR formats, microplates, and other laboratory vessels with appropriate rotor configurations.

With the included S-24-11-AT rotor, this particular centrifuge is configured for swinging-bucket microcentrifugation rather than conventional fixed-angle separation. This makes it particularly useful for laboratories requiring controlled sedimentation of small-volume samples while maintaining refrigerated operating conditions.

The instrument's programmable controls, automatic rotor recognition, and pre-cooling functions also make it suitable for shared laboratory environments where different researchers may require consistent operating parameters.

Common Applications

The Eppendorf 5430R with S-24-11-AT rotor is well suited for laboratory procedures requiring controlled sedimentation of small-volume biological samples.

Common applications include molecular biology research, DNA and RNA sample preparation, protein precipitation, enzyme preparation, cell lysate processing, microbiology research, biotechnology applications, pharmaceutical research, and general refrigerated microcentrifugation.

The swinging-bucket rotor is particularly useful for procedures where pellet location and recovery are important considerations. Refrigeration also makes the centrifuge suitable for protocols involving temperature-sensitive proteins, enzymes, nucleic acids, and other biological materials.

For laboratories performing repeated sample-preparation procedures, the 24-place capacity allows multiple samples to be processed simultaneously while maintaining consistent operating conditions.

Included S-24-11-AT Swinging-Bucket Rotor

This Eppendorf 5430R centrifuge includes the S-24-11-AT aerosol-tight swinging-bucket rotor, designed specifically for 24 standard 1.5/2.0 mL microcentrifuge tubes.

The rotor operates at a maximum speed of 12,700 RPM and generates centrifugal forces up to 16,049 × g.

During centrifugation, the rotor's tube holders swing outward until the tubes are positioned horizontally. This changes the direction of sedimentation compared with a fixed-angle rotor, allowing particles to collect toward the bottom of the vessel.

The S-24-11-AT incorporates Eppendorf's QuickLock rotor lid system, providing convenient access while maintaining an aerosol-tight configuration when the appropriate lid and sealing components are properly installed.

The rotor must always be operated with its designated lid.

Eppendorf specifies that the S-24-11-AT is intended exclusively for 1.5/2.0 mL reaction vessels. Spin columns, smaller tubes, and adapters for 0.2 mL, 0.4 mL, 0.5 mL, and 0.6 mL vessels are not approved for use with this rotor.

Laboratories requiring those sample formats should select another compatible Eppendorf rotor. Customers should verify all included rotor accessories before purchase.

Key Specifications

Specifications compiled from Eppendorf manufacturer literature and technical documentation. Maximum speed and centrifugal force depend on the installed rotor.

Swinging-Bucket Centrifugation and Sample Recovery

The S-24-11-AT provides a different sedimentation geometry from the fixed-angle rotors commonly supplied with high-speed microcentrifuges.

In a fixed-angle rotor, particles typically sediment along the outer wall of the tube before collecting toward the bottom. This can produce a pellet extending along the side of the vessel.

With a swinging-bucket rotor, the tubes move into a horizontal position during operation, directing sedimentation toward the bottom of the tube.

This can be advantageous for procedures where laboratories want more predictable pellet positioning, particularly when recovering supernatant or handling delicate biological material.

For laboratories processing 24 microcentrifuge tubes at a time, the S-24-11-AT provides a useful combination of sample capacity, high centrifugal force, and controlled sedimentation geometry.

Eppendorf 5430R centrifuge Refrigeration and Temperature Stability

The Eppendorf 5430R provides an adjustable temperature range from -11°C to +40°C, allowing laboratories to select operating conditions appropriate for their samples.

High-speed centrifugation generates heat, and temperature increases can affect sensitive biological materials. The 5430R refrigeration system helps maintain controlled chamber conditions during centrifugation.

Its FastTemp function allows the instrument to be pre-cooled before samples are loaded, reducing unnecessary exposure of temperature-sensitive materials to ambient conditions.

The centrifuge also supports FastTemp pro programming, allowing laboratories to schedule pre-cooling around routine operating requirements.

These capabilities are particularly useful for laboratories performing repeated sample-preparation procedures involving proteins, enzymes, nucleic acids, and other biological materials.

Actual sample temperature depends on rotor selection, operating speed, sample load, ambient conditions, and other operating parameters.

Rotor Compatibility and Additional Applications

Although this particular Eppendorf 5430R includes the S-24-11-AT swinging-bucket rotor, the centrifuge supports 12 compatible rotor configurations.

Additional rotor options allow laboratories to process standard microcentrifuge tubes, Eppendorf 5.0 mL tubes, 15 mL and 50 mL conical tubes, PCR tubes and strips, cryotubes, spin columns, and microplates.

With an appropriate high-speed fixed-angle rotor, the centrifuge can reach its maximum platform performance of 17,500 RPM and 30,130 × g.

This versatility allows the 5430R to serve multiple laboratory functions without requiring separate centrifuges for every sample format.

For laboratories that initially require swinging-bucket microtube processing, the ability to add compatible rotors later can extend the instrument's usefulness as research requirements change.

Additional rotors and adapters are not included unless specifically listed. Customers should verify included rotors and accessories before purchase.

Power Requirements and Shipping

The Eppendorf 5430R operates on 120V, 50/60 Hz electrical service, making it suitable for installation in many U.S. laboratory environments.

The centrifuge measures approximately 15.0 inches wide × 25.2 inches deep × 11.7 inches high and weighs approximately 123.5 pounds without a rotor.

Approximately 24.8 inches of vertical clearance is required with the centrifuge lid fully open.

The instrument should be positioned on a sturdy, level laboratory bench with adequate ventilation clearance to support refrigeration performance.

American Instrument Exchange prepares centrifuges for secure shipment, with appropriate protection for the rotor chamber, drive assembly, refrigeration components, controls, and other sensitive parts.

Refurbished Eppendorf Centrifuges from American Instrument Exchange

American Instrument Exchange technicians inspect and test refurbished centrifuges before shipment to verify proper mechanical and electrical operation.

Testing includes evaluation of the drive motor, spindle and bearing condition, rotating assembly balance, braking, electronic controls, lid safety mechanisms, rotor recognition, and overall operating performance.

Because the Eppendorf 5430R is refrigerated, technicians also evaluate the cooling system to verify that the instrument reaches and maintains appropriate operating temperatures.

The included S-24-11-AT rotor is inspected for structural integrity, wear, corrosion, and proper operation. The rotor and centrifuge are tested together as a complete system.

The instrument is thoroughly cleaned and prepared for shipment after testing.

This refurbished Eppendorf 5430R includes a 90-day warranty from American Instrument Exchange.

Service and Support

American Instrument Exchange and AIE Laboratory Services provide diagnostic evaluation, preventative maintenance, and repair support for centrifuges and other laboratory equipment.

For an Eppendorf 5430R, service evaluation may include the motor, spindle assembly, bearings, braking system, refrigeration components, temperature sensors, rotor recognition, electronic controls, and lid safety mechanisms.

Our technicians can help laboratories determine whether repair or replacement with another refurbished centrifuge is the more economical solution.

Related Laboratory Equipment

American Instrument Exchange offers a broad selection of used and refurbished laboratory centrifuges, including Eppendorf microcentrifuges, refrigerated benchtop centrifuges, general-purpose centrifuges, and high-speed floor-model systems.

Laboratories performing molecular biology, biotechnology, and cell culture research may also be interested in our selection of laboratory incubators, laboratory freezers and ultra-low freezers, and biosafety cabinets.

Eppendorf 5430R with S-24-11-AT Rotor Frequently Asked Questions

What rotor is included with this Eppendorf 5430R?

This particular centrifuge includes the Eppendorf S-24-11-AT aerosol-tight swinging-bucket rotor, designed for 24 standard 1.5/2.0 mL microcentrifuge tubes.

What is the maximum speed of the S-24-11-AT rotor?

The S-24-11-AT operates at speeds up to 12,700 RPM and generates centrifugal forces up to 16,049 × g.

Is the S-24-11-AT a fixed-angle or swinging-bucket rotor?

The S-24-11-AT is a swinging-bucket rotor. Its tube holders move into a horizontal position during centrifugation, allowing sedimentation toward the bottom of the tubes.

Is the S-24-11-AT rotor aerosol-tight?

Yes. The S-24-11-AT incorporates an aerosol-tight rotor lid system when the correct lid and sealing components are installed and maintained according to Eppendorf instructions.

Can the S-24-11-AT process spin columns?

No. Eppendorf specifies that spin columns are not approved for use with this rotor. Laboratories processing spin columns should select an appropriate compatible fixed-angle rotor.

Can the S-24-11-AT accommodate smaller microtubes with adapters?

No. The S-24-11-AT is designed exclusively for 1.5/2.0 mL reaction vessels. Adapters for smaller tube sizes are not approved for this rotor.

What is the advantage of a swinging-bucket microtube rotor?

A swinging-bucket rotor positions tubes horizontally during centrifugation, directing sedimentation toward the bottom of the vessel. This can be useful for applications where consistent pellet positioning and sample recovery are important.

What temperature range does the Eppendorf 5430R provide?

The centrifuge offers an adjustable temperature range from -11°C to +40°C. Actual sample temperature depends on operating conditions and rotor selection.

Why is the centrifuge rated for 30,130 × g if the included rotor only reaches 16,049 × g?

The 30,130 × g specification represents the maximum capability of the centrifuge platform with an appropriate high-speed rotor. The included S-24-11-AT is designed for swinging-bucket microtube processing and therefore operates at a lower maximum centrifugal force.

Can the Eppendorf 5430R accommodate 15 mL and 50 mL conical tubes?

Yes. With an appropriate compatible rotor, such as the F-35-6-30, the 5430R can process 15 mL and 50 mL conical tubes. The included S-24-11-AT rotor is designed only for 1.5/2.0 mL tubes.

How does the Eppendorf 5430R compare with the non-refrigerated 5430?

The primary difference is refrigeration. The 5430R provides controlled temperatures from -11°C to +40°C, while the non-refrigerated 5430 is smaller, lighter, and intended for applications that do not require active cooling. Both platforms support a broad selection of compatible rotors.

Why purchase a refurbished Eppendorf 5430R?

A professionally tested refurbished Eppendorf 5430R can provide versatile refrigerated centrifugation at a lower acquisition cost than a new instrument. With the included S-24-11-AT rotor, it is particularly well suited for laboratories requiring high-speed swinging-bucket microtube processing and controlled sample temperatures.