Sartorius Certomat CT Plus Shaking Incubator

Brand : Sartorius

Product Code : SKU 4841L SHAKER

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Sartorius CERTOMAT CT Plus CO2 Incubation Shaker

The Sartorius CERTOMAT CT Plus is a specialized CO2 incubation shaker designed for mammalian cell culture and other biological applications requiring simultaneous control of temperature, CO2 concentration, humidity, and orbital agitation. Unlike a conventional heated incubator shaker, the CERTOMAT CT Plus creates a controlled CO2 incubation environment while continuously shaking culture vessels, making it particularly well suited for suspension cell culture, process development, and scale-up applications.

Available used and refurbished from American Instrument Exchange, this Sartorius CERTOMAT CT Plus is inspected, cleaned, and tested prior to shipment. The unit includes a 90-day warranty from American Instrument Exchange.

Key Features

CO2-controlled incubation shaker for mammalian cell culture
40–400 rpm shaking speed range
Controlled temperature, CO2, and humidity environment
Temperature range from ambient +8°C to 70°C
Fully encapsulated incubation chamber
Separate heating systems help minimize condensation
Programmable operating parameters
Stores up to five programs with four steps plus a pre-step
Memory recovery of operating setpoints following a power interruption
Stainless steel spill tray
Analog outputs for continuous parameter recording
115 V, 60 Hz electrical configuration
Refurbished and tested by American Instrument Exchange
90-day warranty included

Overview

The Sartorius CERTOMAT CT Plus was developed to combine the controlled environment of a CO2 incubator with the continuous agitation of an orbital shaker. This makes it fundamentally different from standard incubated shakers intended primarily for bacterial and yeast culture.

Temperature, CO2 concentration, and humidity are controlled within the incubation chamber to support mammalian-cell cultivation and related workflows. The chamber is isolated from the mechanical drive and control components, helping protect these systems from the corrosive conditions that can develop in warm, humid environments containing elevated concentrations of CO2.

Separate heating systems are used to help reduce condensation, while advanced insulation and sealing support stable environmental conditions during extended culture procedures.

The CT Plus is also programmable. Up to five programs can be stored, with as many as four operating steps and a pre-step in each program. This allows researchers to establish multi-stage protocols involving changes in applicable operating parameters over time.

Key Specifications

Specification	Details
Manufacturer	Sartorius
Model	CERTOMAT CT Plus
Equipment Type	CO2 incubation orbital shaker
Primary Application	Mammalian and suspension cell culture
Speed Range	40–400 rpm
Orbital Motion	25 or 50 mm depending on drive configuration; verify individual unit
Temperature Range	Ambient +8°C to 70°C
Environmental Control	Temperature, CO2, and humidity
Programming	Up to 5 programs with 4 steps plus a pre-step
Chamber	Fully encapsulated incubation chamber
Interior Construction	Stainless steel spill tray
Data Output	Analog output for parameter recording
Exterior Dimensions	Approx. 50"W × 27.25"D × 65"H
Electrical	115 V, 60 Hz
Condition	Used / Refurbished
Warranty	90 days from American Instrument Exchange

Sartorius produced CERTOMAT CT Plus systems with different shaking-drive and platform configurations. The orbit, platform, clamps, racks, and vessel arrangement of the individual AIE unit should therefore be confirmed before purchase. Published manufacturer flask capacities should be considered configuration-specific rather than guaranteed capacity for every used CT Plus.

Common Applications

The CERTOMAT CT Plus is particularly well suited for mammalian suspension cell culture, recombinant protein production, monoclonal antibody research, bioprocess development, media optimization, seed-train development, process scale-up studies, and other biological workflows requiring orbital agitation within a controlled CO2 environment.

The ability to combine shaking with controlled CO2, temperature, and humidity can be valuable when moving suspension cultures from smaller incubator-based workflows toward larger shake-flask processes.

Ideal Laboratory Types

The Sartorius CERTOMAT CT Plus is appropriate for biotechnology and biopharmaceutical companies, mammalian cell culture laboratories, cell-line development groups, bioprocess development facilities, protein-expression laboratories, pharmaceutical research organizations, academic research laboratories, and university core facilities.

It can be especially useful for laboratories working with suspension-adapted mammalian cells that need larger-scale orbital culture while maintaining environmental conditions similar to those provided by a conventional CO2 incubator.

CO2, Temperature & Orbital Shaking Performance

The CERTOMAT CT Plus combines orbital shaking from 40 to 400 rpm with controlled temperature, CO2 concentration, and humidity. This integrated design allows researchers to maintain cultures in a controlled incubation environment while continuously agitating the vessels.

Sartorius offered the CT Plus with different orbital-drive configurations, including 25 and 50 mm orbits. Because orbit diameter has a significant effect on liquid movement, mixing intensity, and oxygen transfer, the orbit installed on an individual used system should be verified rather than assumed from the model name alone.

The incubation chamber is isolated from the mechanical drive and control components. This separation is particularly important in a CO2 shaker because moisture and CO2 can combine to create a corrosive environment for exposed mechanical and electronic components.

Separate heating systems also help minimize condensation while maintaining appropriate conditions within the incubation chamber.

As with any cell-culture shaker, actual process performance depends on multiple variables, including shaking speed, orbit diameter, vessel geometry, working volume, cell line, medium formulation, gas conditions, and platform configuration.

Platform, Flask & Vessel Compatibility

The CERTOMAT CT Plus was designed to support large shake-flask culture applications, and manufacturer literature describes configurations capable of accommodating multiple large-volume flasks.

Actual capacity of a used CT Plus, however, depends on the shaking platform and vessel-holding hardware installed on the specific unit. Platform design, flask clamps, vessel diameter, flask geometry, working volume, orbit, spacing, and load distribution can all substantially affect real-world capacity.

For this reason, published capacities such as six 5 L flasks should be treated as examples of particular manufacturer configurations rather than a guaranteed capacity for every CERTOMAT CT Plus.

American Instrument Exchange can confirm the platform, clamps, racks, and other accessories included with the specific unit. Customers intending to use particular shake flasks or other culture vessels should verify compatibility before purchase.

Refurbishment & Testing

Used Sartorius CERTOMAT CT Plus incubation shakers from American Instrument Exchange are inspected and tested prior to shipment. The shaking mechanism is operated for extended periods to verify reliable orbital movement and operation at applicable speed setpoints.

Temperature-controlled operation is independently evaluated using external temperature-measurement equipment to verify chamber performance. Applicable controls, programming functions, displays, alarms, memory functions, and other mechanical and electrical systems are also inspected and tested.

Because the CERTOMAT CT Plus incorporates additional environmental systems beyond those of a conventional incubator shaker, customers with specific requirements for CO2 or humidity operation should discuss those requirements with American Instrument Exchange before shipment. AIE will work to simulate intended operating conditions and verify applicable functions when practical.

The unit is also cleaned and cosmetically prepared as appropriate before shipment.

Shipping & Installation

The CERTOMAT CT Plus is a large floor-standing laboratory system measuring approximately 50 inches wide by 27.25 inches deep by 65 inches high. Laboratories should verify doorway, hallway, elevator, and final installation clearances before delivery.

The shaker should be positioned on a stable, level surface with appropriate clearance for chamber access, ventilation, electrical connections, and service. Because the system controls CO2 as part of the incubation environment, the laboratory should also plan for the appropriate gas supply and connections required by the installed configuration.

This AIE unit is configured for 115 V, 60 Hz electrical service. Gas requirements, shaker orbit, platform configuration, and included accessories should be confirmed for the individual unit before installation.

Related Equipment

Laboratories using the Sartorius CERTOMAT CT Plus may also require CO2 incubators, incubators, centrifuges, laboratory refrigerators, laboratory freezers, ultra-low freezers, and other laboratory shakers. American Instrument Exchange supplies used and refurbished laboratory equipment for biotechnology, biopharmaceutical, academic, clinical, and industrial research laboratories.

Frequently Asked Questions

What is the Sartorius CERTOMAT CT Plus?

The CERTOMAT CT Plus is a CO2-controlled incubation shaker designed to combine orbital agitation with controlled temperature, CO2 concentration, and humidity.

How is the CERTOMAT CT Plus different from the CERTOMAT BS-1?

The BS-1 is primarily a heated incubator shaker. The CT Plus adds CO2 and humidity control, making it specifically suited to mammalian-cell and other CO2-dependent culture applications.

What is the shaking speed range?

The CERTOMAT CT Plus operates from 40 to 400 rpm.

What is the orbit diameter?

Sartorius produced CT Plus systems with 25 mm and 50 mm shaking orbits. The drive configuration of the individual AIE unit should be verified before purchase rather than assuming that one orbit applies to every CT Plus.

What is the temperature range?

The CERTOMAT CT Plus provides temperature control from approximately 8°C above ambient to 70°C.

Does the CERTOMAT CT Plus control CO2?

Yes. CO2 control is one of the defining features of the CT Plus. The system is designed to maintain controlled CO2 conditions along with temperature and humidity for appropriate cell-culture applications.

Can the CERTOMAT CT Plus be used for mammalian cell culture?

Yes. The CT Plus was specifically developed for cultivation applications involving mammalian cells and is particularly useful for suspension culture and bioprocess-development workflows.

Can the CERTOMAT CT Plus hold six 5 L flasks?

Sartorius documentation and published specifications describe configurations capable of accommodating up to six 5 L flasks. However, actual capacity depends on the installed platform, clamps, orbit, vessel geometry, and available clearance. Customers should confirm the configuration of the specific used unit before relying on a published flask count.

Why is the incubation chamber isolated from the shaker drive?

Warm, humid environments containing CO2 can be corrosive to mechanical and electronic components. Separating the incubation chamber from the drive and control systems helps protect these components from the chamber environment.

Can operating programs be stored?

Yes. The CERTOMAT CT Plus can store up to five programs, with four steps and a pre-step available within each program. This allows multi-stage operating protocols to be established and repeated.

What utilities are required for installation?

In addition to appropriate electrical service, CO2 operation requires a suitable external gas supply and connections. Exact utility and connection requirements should be confirmed for the specific CT Plus configuration before installation.

Does American Instrument Exchange test the CERTOMAT CT Plus before shipment?

Yes. AIE tests the shaking mechanism, temperature-controlled operation, controls, programming functions, and other applicable systems. Customers requiring specific CO2 or humidity testing should discuss those requirements with AIE prior to shipment so they can be incorporated into testing when practical.

Does this CERTOMAT CT Plus include a warranty?

Yes. This used and refurbished Sartorius CERTOMAT CT Plus includes a 90-day warranty from American Instrument Exchange.

Testing Procedures for Used and Refurbished Laboratory Equipment

Ensure Accuracy, Safety, and Dependable Performance Across Applications

At American Instrument Exchange, all used and refurbished laboratory equipment undergoes a rigorous, multi-point testing protocol to ensure optimal performance, operational safety, and long-term reliability. Whether the equipment is destined for research, diagnostics, quality control, or production environments, our thorough inspection and testing process is designed to meet the highest standards of laboratory excellence.

Why Testing Used Lab Equipment Is Essential

Before any refurbished laboratory equipment is returned to service, it must be carefully tested to verify:

• Functional integrity and operational performance
• Setpoint accuracy and repeatability (where applicable)
• User interface responsiveness and control features

Proper testing reduces the risk of equipment failure, measurement inaccuracies, or user hazards—helping to ensure consistent results in clinical, academic, biotech, and industrial labs.

Our standardized lab equipment testing procedures are tailored to each equipment type, ensuring every unit meets manufacturer specifications and industry benchmarks.

Key Testing Procedures for Refurbished and Used Laboratory Equipment

1. Initial Visual Inspection

• Examine equipment housing for signs of wear, corrosion, or damage
• Check ports, power cords, and exterior components for tampering or deterioration
• Verify that internal and external parts (racks, shelves, connectors, accessories) are present and undamaged

2. Functional Startup and Operational Testing

• Power on the equipment and verify startup diagnostics
• Confirm functionality of displays, buttons, switches, and control panels
• Run basic functions to confirm proper response and behavior under standard operating conditions

3. Testing and Performance Verification

• Test internal sensors or control systems using industry-standard tools
• Perform operational tests to validate accuracy and consistency
• Verify key performance specifications such as temperature control, timing, speed, or measurement outputs, depending on equipment type

4. Mechanical and Electrical System Testing

• Evaluate motors, pumps, fans, or other moving parts for smooth operation
• Inspect and test wiring, relays, fuses, and circuit boards for safety and reliability
• Test power delivery and grounding compliance

5. Safety and Compliance Checks

• Ensure equipment is grounded and insulated to meet laboratory safety standards
• Test alarms, interlocks, emergency stops, or safety shutdown systems if applicable

6. Final Quality Assurance and Documentation

• Perform extended runtime testing
• Document operational logs, testing results, and any repairs or adjustments made
• Complete a final inspection, thorough cleaning, and packaging for delivery

Trusted Quality from American Instrument Exchange

With over 50 years of experience in laboratory equipment refurbishment, American Instrument Exchange is committed to delivering certified, lab-ready instruments backed by expert testing. Our testing protocols ensure that each piece of equipment—regardless of type—is safe, accurate, and dependable from day one.

Whether you're equipping a startup lab or expanding a high-throughput facility, you can count on our refurbished laboratory equipment to perform with precision and reliability.

Refurbishment Protocol for Used Laboratory Equipment from American Instrument Exchange

At American Instrument Exchange, every piece of used laboratory equipment we offer undergoes a multi-step refurbishment process. Whether it's an analytical balance, centrifuge, freezer, oven, or any other essential lab instrument, our goal is to restore each unit to peak performance—ensuring accuracy, safety, and long-term reliability in scientific, clinical, industrial, and academic environments.

Why Choose Refurbished Lab Equipment from American Instrument Exchange?

• Thoroughly tested for performance, precision, and safety
• Backed by a warranty
• A cost-effective alternative to new equipment
• Environmentally sustainable through equipment reuse and waste reduction
• Serviced by expert technicians with decades of laboratory equipment experience

Step-by-Step Refurbishment Protocol

Every instrument and system refurbished at American Instrument Exchange follows a detailed, industry-aligned process:

1. Initial Intake & Inspection

• Complete visual and structural inspection for cosmetic damage, wear, or corrosion
• Mechanical assessment of hinges, seals, fasteners, motors, and moving parts
• Missing components and damaged items are documented for repair or replacement

2. Cleaning and Decontamination

• Full internal and external cleaning using non-abrasive, lab-safe solutions
• Removal of biological, chemical, or particulate contamination
• Disassembly and cleaning of removable parts such as trays, shelves, and filters

3. Mechanical and Electrical Repair

• Replacement or repair of worn or broken mechanical parts
• Evaluation and servicing of control panels, sensors, switches, and displays
• Testing and repair of electrical wiring, fuses, circuit boards, and power supplies

4. Functional System Testing

• Assessment of all major functions specific to the equipment type (e.g., speed, temperature, force, weight, timing)
• Operation of control interfaces, programming menus, and digital displays
• Verification of stabilization time, responsiveness, and user input reliability

5. Testing and Performance Verification

• Testing of internal sensors, controllers, and measurement systems using certified tools
• Multi-point testing to ensure repeatability, accuracy, and performance consistency
• Confirmation of user-defined setpoints, timing sequences, or programmable cycles

6. Safety and Compliance Checks

• Testing of safety interlocks, alarms, and over-limit protection systems (as applicable)
• Electrical safety inspections, including grounding and insulation verification

7. Final Quality Assurance and Documentation

• Full operational testing under simulated lab conditions
• Completion of performance benchmarks, testing results, and repair logs
• Final cleaning and visual inspection

Trust the Experts at American Instrument Exchange

With over 50 years of experience in the laboratory equipment industry, American Instrument Exchange is a trusted leader in high-quality refurbished lab instruments. Our refurbishment protocols ensure that every piece of equipment we sell is lab-ready—offering the precision, performance, and durability your team can count on.

Whether you’re outfitting a new lab or expanding an existing operation, choose American Instrument Exchange for tested, warrantied, and cost-effective laboratory equipment.

Warranty Information

All used equipment sold by American Instrument Exchange is refurbished/reconditioned, tested to meet specifications, cleaned, and comes with a 90-day warranty from the date of shipment unless otherwise specified. New products include a warranty from the manufacturer. Your warranty is only valid if you have paid your invoice. If your invoice is over 30 days old, warranty work will not be performed until your invoice is paid in full. American Instrument Exchange is not liable for any special or consequential damages that result from the use of, or the inability to use, the materials on this site or the performance of the products,

Exceptions include international sales and items that are agreed to be sold with a lesser warranty or items that are agreed to be sold “as is.”

Should there be visible shipping damage to packaging or concealed shipping damage to item(s) supplied, buyer must communicate such damage to the delivering entity or carrier upon receipt first and to American Instrument the same day item is accepted / signed for. Failure to do so voids warranty. Customer decision to involve / procure third party or OEM service other than American Instrument or AIE assigned vendors to resolve in-warranty instrument failure or defect without first placing a service call to American Instrument voids warranty.

Return & Cancellation Policy

American Instrument Exchange will authorize the return of and issue a full refund for any equipment that is not working as represented and could not be replaced or repaired to the customer’s satisfaction by AIE or a representative of AIE during the warranty period. Returns without prior authorization and an RMA number will not be accepted.

Customers are responsible for proper packaging of the equipment and all included accessories. American Instrument will pay for insurance and shipping costs. Non-inventory items bought specifically to fill customer orders may possibly be returned, but on a case by case basis.

Customers who cancel orders after refurbishing has begun or after delivery where there are no equipment performance issues may be subject to a Restocking/Cancellation Fee of 20% or more and responsible for shipping costs for both the delivery and return.

Ongoing and Extended Support Options

American Instrument Exchange is able to provide additional repair and support services outside the warranty terms for most products that sell.

For biosafety cabinets, clean benches, chemical fume hoods, and other clean air products our team at AIE Laboratory Services can provide certifications, repairs, decontaminations, and other services for customers throughout New England, and guidance and limited technical assistance for any customers in the US and Canada for new or refurbished biosafety cabinets purchased from American Instrument Exchange.

For all other products, the repair service team at American Instrument Exchange can provide both field and depot repair evaluations. We can easily schedule and coordinate the pick-up and transportation of your malfunctioning equipment. By offering hourly rates lower than most OEM service providers and eliminating travel time for technicians, we help reduce repair costs. Furthermore, our lab depot repair service minimizes or eliminates scheduling delays. Our experienced service technicians are qualified to restore your equipment to factory specifications.

Please contact our team at info@americaninstrument.com for more details.

FAQ

Does the equipment come with a warranty?

Yes, all equipment from American Instrument Exchange comes with a warranty.
Used/reconditioned/refurbished equipment is tested to make sure it operates to the manufacturer's specifications and comes with a 90-day warranty (unless otherwise noted). New equipment comes with a warranty from the manufacturer.

How do you test and recondition the equipment?

Testing requirements vary across all the equipment categories we sell. Temperature controlled units are tested with NIST traceable probes and data loggers. Gaskets, external housing, glass, handles, controls and control screens are all thoroughly inspected for integrity and functionality.
We test all equipment using the manufacturer's parameters and specifications to confirm that the unit operates properly.

How do products ship from American Instrument Exchange?

We can ship products both nationally and internationally using a well-established network of transporters. Smaller items will ship via UPS. Larger equipment will be palletized and shipped either LTL or air-ride, depending on the specific equipment and/or delivery requirements.

Return and order cancellation policy

American Instrument Exchange will accept the return of any product that is not working as represented and could not be replaced or repaired to the customer's satisfaction during the warranty period. Customers who cancel orders after refurbishing has begun will be subject to a cancellation and restocking fee of between 20% and 40%.

Are discounts available for educational or non-profit institutions, or for bulk purchases?

Yes – call or email us today with a list of equipment you're looking for and we can potentially put together a quote that includes a discount.

How does your price matching policy work?

We strive to keep our pricing competitive and reasonable. If you find comparable items for sale at a lower price from other vendors please send us a link and or quote for the item and we'll do our best to match or beat the price.

Can I see the equipment in person before committing to a purchase?

Customers are welcome to make an appointment to view equipment in our warehouse. Given our workload we may not be able to test or clean the equipment prior to your arrival if you have not committed to an order.

1023 Western Avenue is a working warehouse, not a showroom, and customers should dress appropriately and wear closed toe shoes.

Can I ship products that I purchased elsewhere to American Instrument for repair?

Yes – please see our repair services page for more information.