VWR 5473 ultralow chest freezer

Brand : VWR

Product Code : SKU 2562R FREEZER

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VWR 5473 Ultra-Low Temperature Chest Freezer

For laboratories responsible for preserving valuable biological collections, temperature stability is every bit as important as storage capacity. The VWR 5473 Ultra-Low Temperature Chest Freezer combines dependable -86°C performance with the inherent thermal advantages of a chest freezer, making it an excellent choice for long-term storage of sensitive biological materials where minimizing temperature fluctuations is a priority.

Designed for biobanks, biotechnology companies, pharmaceutical manufacturers, hospitals, universities, and research institutions, the VWR 5473 provides large-capacity ultra-low temperature storage in a cabinet engineered to retain cold air during routine access. The chest configuration helps reduce cold air loss compared to upright freezers, supporting stable storage conditions for irreplaceable research samples while providing generous capacity for organized long-term inventory.

Many laboratories integrate a chest ultra-low freezer like the VWR 5473 into a complete cold storage program alongside laboratory refrigerators for refrigerated media and reagents, laboratory freezers for routine frozen storage, cryogenic storage systems for liquid nitrogen preservation, and biosafety cabinets for sterile sample preparation.

VWR 5473 Key Features

Ultra-low temperature operation from -50°C to -86°C
Chest freezer configuration for improved cold-air retention
Cascade refrigeration system
Microprocessor temperature controller
Advanced audible and visual alarm system
Automatic voltage compensation
Password-protected controls
Lockable lid
Remote alarm contacts
Refurbished and performance tested by American Instrument Exchange
90-day warranty included

VWR 5473 Overview

The VWR 5473 was developed for laboratories where long-term sample preservation takes precedence over frequent day-to-day access. Unlike upright freezers, the chest design naturally retains dense cold air when the lid is opened, helping reduce temperature fluctuations throughout the storage chamber.

The freezer combines proven cascade refrigeration with microprocessor controls and comprehensive alarm monitoring to provide dependable ultra-low performance for demanding laboratory environments. Automatic voltage compensation helps protect the refrigeration system from power fluctuations, while integrated alarms and remote monitoring capability provide additional confidence when storing valuable biological collections.

Common Applications

The VWR 5473 is commonly used for storing stem cells, cell lines, DNA and RNA libraries, PBMCs, plasma and serum, tissue specimens, blood products, vaccines, protein samples, pharmaceutical compounds, clinical trial materials, long-term research collections, and more.

Its chest configuration makes it particularly attractive for archival storage where samples remain undisturbed for extended periods.

Ideal Laboratory Types

The VWR 5473 is well suited for:

Biotechnology companies
Pharmaceutical manufacturers
Academic research institutions
Hospitals
Clinical laboratories
Public health laboratories
Biobanks
Government research facilities
Clinical research organizations

Laboratories maintaining large biological repositories will benefit from the freezer's emphasis on thermal stability and dependable long-term storage.

Chest Freezer Advantages & Sample Protection

One of the greatest advantages of a chest ultra-low freezer is its ability to retain cold air during routine access. Because cold air naturally remains inside the cabinet when the lid is opened, stored samples experience less thermal disturbance than in many upright freezers.

This design is particularly valuable for laboratories preserving long-term research collections, tissue archives, and other irreplaceable biological materials where minimizing temperature fluctuations contributes to sample integrity over many years.

Typical Stored Materials

The VWR 5473 is commonly used to preserve stem cells, cell lines, PBMCs, DNA and RNA libraries, plasma, serum, tissue specimens, vaccines, protein samples, pharmaceutical compounds, and other biological collections requiring storage as low as -86°C.

Applications requiring temperatures approaching -196°C are generally better suited for dedicated cryogenic storage systems utilizing liquid nitrogen.

VWR 5473 Key Specifications

Temperature Range

Adjustable from -50°C to -86°C

Refrigeration System

Cascade refrigeration
Air-cooled system

Monitoring & Controls

Microprocessor controller
Audible and visual alarms
Automatic voltage compensator
Password-protected settings
Remote alarm capability

Security

Lockable lid

Electrical Requirements

Refer to manufacturer specifications for the installed voltage configuration.

Operating Costs & Energy Considerations

Like most traditional chest ultra-low freezers, the VWR 5473 utilizes a cascade refrigeration system designed for dependable long-term operation. Its chest configuration helps reduce cold air loss during access, which can improve temperature recovery and reduce compressor workload over time. Routine condenser cleaning, adequate ventilation, and preventative maintenance are essential for maximizing cooling efficiency and extending equipment life.

Specifications compiled from VWR manufacturer literature and American Instrument Exchange product information.

Refurbishment & Testing Process

Every VWR 5473 received by American Instrument Exchange undergoes a comprehensive inspection and extended performance evaluation before shipment. Our technicians verify pull-down performance, temperature stability, refrigeration operation, controller functionality, alarm systems, lid seals, and overall cabinet condition before thoroughly cleaning the freezer for delivery.

Laboratories building complete cold storage programs frequently purchase laboratory refrigerators, laboratory freezers, cryogenic storage systems, biosafety cabinets, and centrifuges from American Instrument Exchange.

Shipping & Installation Considerations

The VWR 5473 ships by freight from American Instrument Exchange. Before installation, laboratories should verify floor loading capacity, electrical service, available floor space, doorway clearances, and adequate ventilation around the cabinet. Planning rack layout and sample organization before installation can significantly improve retrieval efficiency and reduce lid-open time.

Frequently Asked Questions

Why choose a chest ultra-low freezer instead of an upright model?

Chest ultra-low freezers naturally retain cold air when opened, helping reduce temperature fluctuations during sample retrieval and making them an excellent choice for long-term archival storage.

What types of samples are commonly stored in the VWR 5473?

The freezer is commonly used for stem cells, cell lines, tissue specimens, plasma, serum, DNA and RNA libraries, vaccines, pharmaceutical materials, and other biological collections requiring storage down to -86°C.

Does the VWR 5473 support remote monitoring?

Yes. The freezer includes remote alarm capability, allowing integration with facility monitoring systems for additional sample protection.

How is the freezer tested before shipment?

Every refurbished VWR 5473 is inspected, cleaned, and performance tested by American Instrument Exchange technicians to verify refrigeration performance, temperature stability, controller operation, alarms, and overall system reliability before shipment.

Testing Procedures for Used and Refurbished Ultralow Freezers
Ensure -80°C Stability and Critical Sample Protection

Used and refurbished ultralow freezers from American Instrument Exchange are rigorously tested to ensure reliable long-term storage at temperatures as low as -80°C. Each unit undergoes a detailed inspection, full system evaluation, and extended runtime testing to meet the high standards required by research, clinical, and biorepository environments.

Why Testing Used Ultralow Freezers Is Essential

Before an ultralow freezer is placed back into service, it must be carefully tested for:
• Temperature stability and uniformity at ultralow levels (e.g., -70°C to -86°C)
• Cascade refrigeration system functionality and compressor health
• Seal and insulation effectiveness to prevent thermal loss
• Electrical safety, alarm functionality, and system compliance

Thorough testing helps prevent temperature excursions and equipment failure—crucial for protecting sensitive materials such as biological samples, vaccines, enzymes, and research specimens.
Our structured ULT testing protocol ensures each refurbished unit delivers dependable performance and long-term reliability.

Key Testing Procedures for Refurbished and Used Ultralow Freezers

1. Initial Visual Inspection

• Inspect external cabinet, hinges, and door gaskets for wear, corrosion, or damage
• Check power cords, plugs, and control interfaces for signs of wear or tampering
• Verify that internal racks, shelving, and chamber components are intact and operational

2. Functional Startup and Control Testing

• Power on the unit and confirm successful initialization of digital displays and alarm systems
• Test control panels, keypad responsiveness, and user-setpoint functionality
• Verify that door seals close tightly and form a secure barrier

3. Temperature and -80°C Stability Testing

• Test and evaluate internal temperature sensors thermometers/probes
• Monitor temperature performance at multiple chamber points over extended runtimes
• Confirm uniformity and stability at key setpoints, including -70°C, -80°C, and -86°C

4. Refrigeration System and Cascade Compressor Evaluation

• Test dual-stage (cascade) compressor function, startup times, and cycling behavior
• Evaluate evaporator coils, heat exchangers, and vacuum insulation panels for performance and integrity
• Check for refrigerant leaks, low-pressure faults, and abnormal compressor noise

5. Alarm and Safety System Verification

• Test high/low temperature alarms, power failure alerts, and battery backup systems (if equipped)
• Verify lockout controls, alarm delay settings, and door-open indicators

6. Final Quality Assurance and Documentation

• Run a continuous multi-day test cycle at -80°C to confirm sustained performance
• Document temperature logs, controller accuracy, and compressor behavior
• Complete a detailed service report

Dependable Performance from American Instrument Exchange

All refurbished ultralow freezers from American Instrument Exchange are tested to exacting standards for performance, safety, and reliability. With our rigorous inspection and testing procedures, you can trust your ULT freezer to deliver consistent -80°C storage from day one—ideal for research, clinical, pharmaceutical, and biobanking applications.

Repair and Refurbishment Protocol for Used Ultralow Freezers
-80°C Storage Performance from American Instrument Exchange

At American Instrument Exchange, every used ultralow temperature freezer (ULT freezer) undergoes a rigorous, multi-step refurbishment protocol designed to restore reliable -80°C performance. Whether upright, chest-style, or undercounter, our refurbished ULT freezers are tested and restored to deliver the cold storage precision required for biological samples, vaccines, enzymes, and other temperature-sensitive materials.

Why Choose a Refurbished Ultralow Freezer from American Instrument Exchange?

• Fully tested for -80°C cold storage stability and compressor performance
• Backed by a warranty
• A cost-effective alternative to expensive new ULT units
• Environmentally responsible through equipment reuse and waste reduction
• Serviced by experienced technicians specializing in laboratory-grade refrigeration

Step-by-Step Ultralow Freezer Refurbishment Protocol

Every ultralow freezer sold by American Instrument Exchange is carefully evaluated and restored through the following process:

1. Initial Intake & Inspection

• Exterior inspection for dents, corrosion, or physical damage
• Evaluation of door seals, hinges, and internal chamber components
• Documentation of missing parts or components requiring repair

2. Cleaning and Decontamination

• Comprehensive interior and exterior cleaning using non-abrasive cleaners
• Removal of dust, frost buildup, and residual contaminants
• Decontamination of all internal surfaces and removable parts like shelves and drawers

3. Mechanical and Electrical Repairs

• Replacement or repair of worn or damaged components (door latches, hinges, gaskets, etc.)
• Inspection and repair of control boards, displays, sensors, and switches
• Electrical system evaluation, including fuses, wiring, relays, and connectors

4. Ultralow Refrigeration System Assessment

• Testing of dual-stage compressors and cascade cooling system
• Evaluation of heat exchangers, evaporator coils, and vacuum insulation panels
• Inspection for refrigerant leaks, low pressure, or signs of compressor fatigue
• Verification of thermal insulation integrity

5. Temperature and Performance Testing

• Testing of internal temperature sensors and controllers
• Multi-point testing to verify chamber temperature uniformity at -80°C
• Long-duration, multi-day operational testing to ensure sustained ultralow performance

6. Safety and Compliance Checks

• Electrical safety testing and grounding verification
• Alarm function tests for high/low temperature alerts and power failure warnings

7. Final Quality Control and Documentation

• Multi-day runtime validation at -80°C
• Recording of temperature logs, compressor cycle data, and performance benchmarks
• Final inspection and service/testing report completed

Trust the Experts at American Instrument Exchange

With over 50 years in the lab equipment industry, American Instrument Exchange is your trusted source for refurbished ultralow freezers. Our certified refurbishment protocol ensures each ULT freezer delivers the precision, performance, and reliability your lab needs—at a fraction of the cost of new equipment.

Warranty Information

All used equipment sold by American Instrument Exchange is refurbished/reconditioned, tested to meet specifications, cleaned, and comes with a 90-day warranty from the date of shipment unless otherwise specified. New products include a warranty from the manufacturer. Your warranty is only valid if you have paid your invoice. If your invoice is over 30 days old, warranty work will not be performed until your invoice is paid in full. American Instrument Exchange is not liable for any special or consequential damages that result from the use of, or the inability to use, the materials on this site or the performance of the products,

Exceptions include international sales and items that are agreed to be sold with a lesser warranty or items that are agreed to be sold “as is.”

Should there be visible shipping damage to packaging or concealed shipping damage to item(s) supplied, buyer must communicate such damage to the delivering entity or carrier upon receipt first and to American Instrument the same day item is accepted / signed for. Failure to do so voids warranty. Customer decision to involve / procure third party or OEM service other than American Instrument or AIE assigned vendors to resolve in-warranty instrument failure or defect without first placing a service call to American Instrument voids warranty.

Return & Cancellation Policy

American Instrument Exchange will authorize the return of and issue a full refund for any equipment that is not working as represented and could not be replaced or repaired to the customer’s satisfaction by AIE or a representative of AIE during the warranty period. Returns without prior authorization and an RMA number will not be accepted.

Customers are responsible for proper packaging of the equipment and all included accessories. American Instrument will pay for insurance and shipping costs. Non-inventory items bought specifically to fill customer orders may possibly be returned, but on a case by case basis.

Customers who cancel orders after refurbishing has begun or after delivery where there are no equipment performance issues may be subject to a Restocking/Cancellation Fee of 20% or more and responsible for shipping costs for both the delivery and return.

Ongoing and Extended Support Options

American Instrument Exchange is able to provide additional repair and support services outside the warranty terms for most products that sell.

For biosafety cabinets, clean benches, chemical fume hoods, and other clean air products our team at AIE Laboratory Services can provide certifications, repairs, decontaminations, and other services for customers throughout New England, and guidance and limited technical assistance for any customers in the US and Canada for new or refurbished biosafety cabinets purchased from American Instrument Exchange.

For all other products, the repair service team at American Instrument Exchange can provide both field and depot repair evaluations. We can easily schedule and coordinate the pick-up and transportation of your malfunctioning equipment. By offering hourly rates lower than most OEM service providers and eliminating travel time for technicians, we help reduce repair costs. Furthermore, our lab depot repair service minimizes or eliminates scheduling delays. Our experienced service technicians are qualified to restore your equipment to factory specifications.

Please contact our team at info@americaninstrument.com for more details.

FAQ

Does the equipment come with a warranty?

Yes, all equipment from American Instrument Exchange comes with a warranty.
Used/reconditioned/refurbished equipment is tested to make sure it operates to the manufacturer's specifications and comes with a 90-day warranty (unless otherwise noted). New equipment comes with a warranty from the manufacturer.

How do you test and recondition the equipment?

Testing requirements vary across all the equipment categories we sell. Temperature controlled units are tested with NIST traceable probes and data loggers. Gaskets, external housing, glass, handles, controls and control screens are all thoroughly inspected for integrity and functionality.
We test all equipment using the manufacturer's parameters and specifications to confirm that the unit operates properly.

How do products ship from American Instrument Exchange?

We can ship products both nationally and internationally using a well-established network of transporters. Smaller items will ship via UPS. Larger equipment will be palletized and shipped either LTL or air-ride, depending on the specific equipment and/or delivery requirements.

Return and order cancellation policy

American Instrument Exchange will accept the return of any product that is not working as represented and could not be replaced or repaired to the customer's satisfaction during the warranty period. Customers who cancel orders after refurbishing has begun will be subject to a cancellation and restocking fee of between 20% and 40%.

Are discounts available for educational or non-profit institutions, or for bulk purchases?

Yes – call or email us today with a list of equipment you're looking for and we can potentially put together a quote that includes a discount.

How does your price matching policy work?

We strive to keep our pricing competitive and reasonable. If you find comparable items for sale at a lower price from other vendors please send us a link and or quote for the item and we'll do our best to match or beat the price.

Can I see the equipment in person before committing to a purchase?

Customers are welcome to make an appointment to view equipment in our warehouse. Given our workload we may not be able to test or clean the equipment prior to your arrival if you have not committed to an order.

1023 Western Avenue is a working warehouse, not a showroom, and customers should dress appropriately and wear closed toe shoes.

Can I ship products that I purchased elsewhere to American Instrument for repair?

Yes – please see our repair services page for more information.