All products ship from our 30,000 sq. ft. Haverhill, MA warehouse
Thermo Forma Series II 3110 CO₂ Incubator
The Thermo Forma Series II 3110 CO₂ Incubator is a full-size, water-jacketed cell culture incubator designed to provide stable temperature, CO₂, and humidity conditions for routine and demanding laboratory applications. With a 184 L (6.5 cu. ft.) chamber, the Series II 3110 provides substantially more culture capacity than compact benchtop incubators while maintaining the environmental consistency required for mammalian cell culture, pharmaceutical research, biotechnology, and other life science workflows.
Available used and refurbished from American Instrument Exchange, the Thermo Forma 3110 remains a practical choice for laboratories seeking a proven CO₂ incubation platform without the cost of purchasing a new system. Its water-jacketed chamber helps maintain temperature stability during routine operation and temporary power interruptions, while thermal conductivity CO₂ control, high-humidity operation, stainless steel construction, and adjustable shelving provide a versatile environment for day-to-day cell culture.
Key Features
184 L (6.5 cu. ft.) chamber capacity
Water-jacketed design for excellent temperature stability
Temperature range from 5°C above ambient to 55°C
±0.1°C temperature control
±0.2°C temperature uniformity at 37°C
0–20% CO₂ control range
Thermal conductivity CO₂ sensor
High-humidity operation for reduced media evaporation
Polished stainless steel chamber
User-programmable high/low temperature and CO₂ alarms
115V, 50/60 Hz electrical configuration
Professionally inspected and tested by American Instrument Exchange
90-day warranty included
Thermo Forma Series II 3110 CO₂ Incubator Overview
The Thermo Forma Series II 3110 is designed around one of the most established approaches to CO₂ incubation: a water jacket surrounding the culture chamber. The thermal mass of the water helps buffer the interior against temperature fluctuations, providing a stable environment for cultures during normal laboratory operation and helping slow temperature loss if electrical power is temporarily interrupted.
With a 184-liter chamber, the 3110 offers enough capacity for laboratories maintaining multiple cell lines or larger quantities of culture vessels without requiring a reach-in incubator. The chamber can accommodate flasks, plates, dishes, and other commonly used culture vessels while adjustable shelving allows researchers to organize the interior according to individual workflows.
The Model 3110 uses a thermal conductivity (TC) CO₂ sensor and provides CO₂ control from 0–20%. Temperature is controlled using a precision thermistor, with specified temperature uniformity of ±0.2°C at 37°C. Together with passive humidification, these systems provide the warm, humidified, CO₂-controlled environment required for routine mammalian cell culture.
For laboratories replacing an older Forma incubator, adding culture capacity, or seeking a dependable secondary incubator, the Series II 3110 continues to offer a straightforward combination of chamber capacity, environmental stability, and proven laboratory design.
Common Applications
The Thermo Forma Series II 3110 is well suited for routine cell culture applications in academic, biotechnology, pharmaceutical, clinical research, and industrial laboratories. Typical uses include mammalian cell culture, cancer research, immunology, vaccine development, molecular and cellular biology, tissue culture, pharmaceutical research, primary cell work, hybridoma production, and other applications requiring controlled temperature, humidity, and CO₂.
The relatively large 184 L chamber also makes the 3110 a good fit for shared laboratories and research groups maintaining multiple cultures simultaneously. Laboratories can dedicate individual shelves to separate projects or researchers while maintaining all cultures within the same controlled environment.
Cell culture workflows frequently pair a CO₂ incubator with biosafety cabinets for aseptic manipulation, laboratory centrifuges for cell harvesting and sample preparation, and laboratory refrigerators for media and reagent storage. For longer-term preservation of cells and biological materials, American Instrument Exchange also offers a wide selection of ultra-low temperature freezers.
Cell Types & Culture Recommendations
The Thermo Forma 3110 can support a broad range of CO₂-dependent cultures when operated within the environmental requirements established for the specific protocol. Its larger chamber makes it especially useful for laboratories running multiple cultures or experiments concurrently.
Common Cell Types
Mammalian cell lines
Primary cells
Fibroblasts
Epithelial cells
Immune cells
Hybridoma cultures
Cancer cell lines
Common biotechnology and pharmaceutical research cell lines
Typical Culture Vessels
T-flasks
Multiwell plates
Cell culture dishes
Petri dishes
Suspension culture flasks
Media bottles
Other standard cell culture vessels compatible with the chamber and shelving
For laboratories with higher culture volumes, the 184 L chamber provides considerably more flexibility than a compact CO₂ incubator. Researchers can organize different cell lines, experiments, or culture vessels across multiple shelves while retaining adequate space for air circulation throughout the chamber.
Environmental Control & Contamination Protection
One of the defining features of the Thermo Forma Series II 3110 is its water-jacketed temperature-control system. Water surrounding the chamber provides substantial thermal mass, helping resist rapid temperature changes and maintain a stable environment for sensitive cultures. This can be particularly valuable during brief power interruptions or in laboratories where consistent long-term incubation conditions are a priority.
The Model 3110 provides a temperature range from approximately 5°C above ambient to 55°C, with ±0.1°C control and specified uniformity of ±0.2°C at 37°C. An independent over-temperature protection circuit provides an additional safeguard against excessive chamber temperatures.
CO₂ concentration is adjustable from 0–20% and monitored by a thermal conductivity sensor. User-programmable high and low alarms alert laboratory personnel to deviations in temperature or CO₂ concentration, helping protect cultures from unexpected environmental changes.
Passive humidification using a removable water pan can provide high relative humidity at typical cell culture temperatures, helping reduce evaporation from culture media. The polished stainless steel chamber provides a durable, corrosion-resistant interior that can be cleaned as part of routine contamination-control procedures.
The combination of water-jacketed temperature stability, controlled CO₂ delivery, humidification, alarms, and a cleanable stainless steel interior makes the Series II 3110 a dependable platform for laboratories performing routine and long-duration cell culture.
Thermo Forma Series II 3110 CO₂ Incubator Specifications
Specification
Details
Manufacturer
Thermo Scientific / Forma
Model
Series II 3110
Equipment Type
Water-Jacketed CO₂ Incubator
Chamber Capacity
184 L (6.5 cu. ft.)
Temperature Range
5°C above ambient to 55°C
Temperature Control
±0.1°C
Temperature Uniformity
±0.2°C at 37°C
Temperature Sensor
Precision thermistor
CO₂ Range
0–20%
CO₂ Control
Better than ±0.1%
CO₂ Sensor
Thermal conductivity (TC)
O₂ Control
None
Relative Humidity
Up to approximately 95% RH at 37°C
Humidity Source
Removable 3 L (0.8 gal.) humidity pan
Chamber Material
Polished stainless steel
Interior Dimensions (W × H × D)
21.3" × 26.8" × 20"
Electrical
115/120V, 50/60 Hz
Alarms
User-programmable high/low temperature and CO₂
Over-Temperature Protection
Independent circuit
Approximate Weight
365 lb.
Specifications may vary according to production date, configuration, and installed options. Confirm the specifications and included accessories of the individual incubator before purchase.
Thermo Forma Series II 3110 CO₂ Incubator Frequently Asked Questions
What is the advantage of a water-jacketed CO₂ incubator?
A water jacket adds substantial thermal mass around the incubation chamber, helping maintain stable temperatures and slowing temperature changes during temporary power interruptions. This makes water-jacketed incubators particularly attractive for laboratories performing long-term cell culture where environmental stability is important.
What is the chamber capacity of the Thermo Forma 3110?
The Series II 3110 provides approximately 184 L (6.5 cu. ft.) of chamber capacity, making it a full-size CO₂ incubator suitable for laboratories maintaining multiple cultures or experiments simultaneously.
What type of CO₂ sensor does the Forma 3110 use?
The Model 3110 uses a thermal conductivity (TC) CO₂ sensor. Other models in the Series II family were available with infrared sensors and oxygen-control capabilities, so buyers comparing similar Forma incubators should verify the exact model number and configuration.
Is the Thermo Forma 3110 suitable for mammalian cell culture?
Yes. Its temperature control, 0–20% CO₂ range, humidified chamber, and 184 L capacity make the 3110 well suited for many routine mammalian cell culture applications. Researchers should always verify that their specific culture protocol falls within the incubator's operating specifications.
Does the Thermo Forma 3110 provide oxygen control?
No. The Model 3110 is a temperature- and CO₂-controlled incubator without O₂ control. Other models within the Forma Series II 3100 family were produced with oxygen-control capabilities for applications requiring hypoxic or hyperoxic conditions.
Is this Thermo Forma Series II 3110 refurbished?
Yes. Used Thermo Forma Series II 3110 incubators offered by American Instrument Exchange are inspected and tested prior to shipment and include a 90-day warranty. Included shelves, accessories, and individual unit configurations should be confirmed before purchase.
Click Hereto see the Thermo Forma Series II incubator on the American Instrument Exchange YouTube channel.
Click Hereto read a review of this product on our blog!
Testing Procedures for Used and Refurbished Laboratory Incubators Ensure Quality and Stability Before Use
Used and refurbished laboratory incubators from American Instrument Exchange are thoroughly tested to ensure optimal performance and temperature stability with laboratory safety standards. Each unit undergoes a detailed inspection and functionality assessment to meet the needs of research, clinical, and industrial labs.
Why Testing Used Laboratory Incubators Is Essential
Before a laboratory incubator is reintroduced into any work environment, it must be tested for:
• Temperature uniformity and accuracy
• Airflow and humidity control (if applicable)
• Door seal integrity and insulation effectiveness
• Electrical and safety system compliance
Comprehensive testing reduces the risk of culture loss, contamination, and equipment failure—especially critical in applications involving cell culture, microbiology, pharmaceutical research, and diagnostics.
Our multi-point testing protocol for used and refurbished incubators ensures reliability, safety, and performance across all models and brands.
Key Testing Procedures for Refurbished and Used Laboratory Incubators
1. Initial Visual Inspection
• Check for cosmetic damage, corrosion, and wear to exterior surfaces, door hinges, and gasket seals
• Inspect power cord, wiring, and display panel for any defects or signs of tampering
• Confirm that shelves, chamber components, and internal fixtures are present and undamaged
2. Functional Testing
• Power on the incubator and verify all display indicators and alarms function correctly
• Test door latch and seal mechanisms for proper closure and insulation
• Run basic temperature cycles to ensure the unit maintains stability over time
3. Temperature Accuracy and Uniformity Tests
• Test internal sensors and controllers using temperature probes
• Measure temperature consistency at multiple chamber locations over several hours
• Confirm that programmable temperature settings respond accurately
4. Airflow and Humidity Control (if applicable)
• Test internal fan operation and airflow distribution
• For humidified incubators, inspect and test humidity reservoirs, sensors, and control systems
• Check for condensation buildup and confirm environmental stability
5. Electrical Safety and Compliance Checks
• Ensure all electrical components are properly grounded
• Perform insulation resistance and leakage current tests
• Verify compliance with current electrical safety standards for laboratory equipment
6. Final Quality Assurance Checklist
• Perform full cycle testing at various temperature setpoints
• Document performance benchmarks and results
All refurbished incubators from American Instrument Exchange are backed by our commitment to quality and precision. With our strict testing procedures, you can rely on your incubator to deliver dependable results from day one.
Repair and Refurbishment Protocol for Used Laboratory Incubators Performance and Quality from American Instrument Exchange
At American Instrument Exchange, every used laboratory incubator we sell goes through a repair and refurbishment protocol. Whether it’s a standard, CO₂, or refrigerated incubator, our goal is to restore each unit to laboratory-level performance—ensuring temperature accuracy, environmental stability, and long-term reliability in your lab.
Why Choose a Refurbished Laboratory Incubator from American Instrument Exchange?
• Fully tested for performance and safety
• Backed by a warranty
• Cost-effective alternative to new equipment
• Environmentally sustainable through reuse and restoration
• Refurbished by expert technicians with decades of lab equipment experience
Step-by-Step Refurbishment Protocol
Every incubator at American Instrument Exchange follows this comprehensive, multi-stage process:
1. Initial Intake & Inspection
• Visual inspection for cosmetic wear, corrosion, dents, or broken components
• Mechanical assessment of door hinges, gaskets, seals, and chamber components
• Document any missing parts or issues requiring repair or replacement
2. Cleaning and Decontamination
• Thorough internal and external cleaning using non-abrasive solutions
• Decontamination/cleaning of surfaces to remove biological, chemical, or particulate residue
• Cleaning of removable parts (e.g., shelves, trays, etc.)
3. Mechanical and Electrical Repair
• Repair or replacement of worn mechanical components such as door latches, fans, hinges, and seals
• Evaluation and repair of control panels, switches, thermostats, and displays
• Inspection and testing of wiring, fuses, relays, and power supplies
4. Environmental Control System Assessment
• For CO₂ incubators: CO₂ sensors, regulators, and gas delivery systems are tested
• For refrigerated incubators: compressors, condensers, and cooling systems are evaluated for performance and leaks
• Fan motors and airflow systems are tested to ensure uniform environmental conditions
5. Temperature and Sensor Testing
• All temperature sensors and controllers are tested using thermometers/probes
• Uniformity and accuracy tested at multiple chamber locations
• For humidified or CO₂ incubators, humidity and CO₂ stability are verified
6. Safety System Testing
• Test of over-temperature protection systems and alarms
• Evaluation of door sensors, safety interlocks, and power failure recovery
7. Final Quality Control and Documentation
• Full performance test run over 24 hours
• Validation of setpoints for temperature, CO₂ (if applicable), and humidity (if applicable)
• Final inspection and cleaning before packaging
• Complete service report
Trust the Experts at American Instrument Exchange
With over 50 years in the lab equipment industry, American Instrument Exchange is a trusted source for high-quality refurbished laboratory incubators. Our proven refurbishment protocol ensures every unit is reliable, accurate, and ready to meet the demands of your lab from day one.
Warranty Information
All used equipment sold by American Instrument Exchange is refurbished/reconditioned, tested to meet specifications, cleaned, and comes with a 90-day warranty from the date of shipment unless otherwise specified. New products include a warranty from the manufacturer. Your warranty is only valid if you have paid your invoice. If your invoice is over 30 days old, warranty work will not be performed until your invoice is paid in full. American Instrument Exchange is not liable for any special or consequential damages that result from the use of, or the inability to use, the materials on this site or the performance of the products,
Exceptions include international sales and items that are agreed to be sold with a lesser warranty or items that are agreed to be sold “as is.”
Should there be visible shipping damage to packaging or concealed shipping damage to item(s) supplied, buyer must communicate such damage to the delivering entity or carrier upon receipt first and to American Instrument the same day item is accepted / signed for. Failure to do so voids warranty. Customer decision to involve / procure third party or OEM service other than American Instrument or AIE assigned vendors to resolve in-warranty instrument failure or defect without first placing a service call to American Instrument voids warranty.
Return & Cancellation Policy
American Instrument Exchange will authorize the return of and issue a full refund for any equipment that is not working as represented and could not be replaced or repaired to the customer’s satisfaction by AIE or a representative of AIE during the warranty period. Returns without prior authorization and an RMA number will not be accepted.
Customers are responsible for proper packaging of the equipment and all included accessories. American Instrument will pay for insurance and shipping costs. Non-inventory items bought specifically to fill customer orders may possibly be returned, but on a case by case basis.
Customers who cancel orders after refurbishing has begun or after delivery where there are no equipment performance issues may be subject to a Restocking/Cancellation Fee of 20% or more and responsible for shipping costs for both the delivery and return.
Ongoing and Extended Support Options
American Instrument Exchange is able to provide additional repair and support services outside the warranty terms for most products that sell.
For biosafety cabinets, clean benches, chemical fume hoods, and other clean air products our team at AIE Laboratory Services can provide certifications, repairs, decontaminations, and other services for customers throughout New England, and guidance and limited technical assistance for any customers in the US and Canada for new or refurbished biosafety cabinets purchased from American Instrument Exchange.
For all other products, the repair service team at American Instrument Exchange can provide both field and depot repair evaluations. We can easily schedule and coordinate the pick-up and transportation of your malfunctioning equipment. By offering hourly rates lower than most OEM service providers and eliminating travel time for technicians, we help reduce repair costs. Furthermore, our lab depot repair service minimizes or eliminates scheduling delays. Our experienced service technicians are qualified to restore your equipment to factory specifications.
Yes, all equipment from American Instrument Exchange comes with a warranty. Used/reconditioned/refurbished equipment is tested to make sure it operates to the manufacturer's specifications and comes with a 90-day warranty (unless otherwise noted). New equipment comes with a warranty from the manufacturer.
How do you test and recondition the equipment?
Testing requirements vary across all the equipment categories we sell. Temperature controlled units are tested with NIST traceable probes and data loggers. Gaskets, external housing, glass, handles, controls and control screens are all thoroughly inspected for integrity and functionality. We test all equipment using the manufacturer's parameters and specifications to confirm that the unit operates properly.
How do products ship from American Instrument Exchange?
We can ship products both nationally and internationally using a well-established network of transporters. Smaller items will ship via UPS. Larger equipment will be palletized and shipped either LTL or air-ride, depending on the specific equipment and/or delivery requirements.
Return and order cancellation policy
American Instrument Exchange will accept the return of any product that is not working as represented and could not be replaced or repaired to the customer's satisfaction during the warranty period. Customers who cancel orders after refurbishing has begun will be subject to a cancellation and restocking fee of between 20% and 40%.
Are discounts available for educational or non-profit institutions, or for bulk purchases?
Yes – call or email us today with a list of equipment you're looking for and we can potentially put together a quote that includes a discount.
How does your price matching policy work?
We strive to keep our pricing competitive and reasonable. If you find comparable items for sale at a lower price from other vendors please send us a link and or quote for the item and we'll do our best to match or beat the price.
Can I see the equipment in person before committing to a purchase?
Customers are welcome to make an appointment to view equipment in our warehouse. Given our workload we may not be able to test or clean the equipment prior to your arrival if you have not committed to an order.
1023 Western Avenue is a working warehouse, not a showroom, and customers should dress appropriately and wear closed toe shoes.
Can I ship products that I purchased elsewhere to American Instrument for repair?
Yes – please see our repair services page for more information.
