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Thermo Steri-Cult 3307 CO₂ Incubator
Successful cell culture depends on more than maintaining the correct temperature and CO₂ concentration. Protecting valuable cultures from airborne contamination while providing a stable incubation environment is equally important, particularly when working with primary cells, stem cells, hybridomas, or long-term research projects. The Thermo Steri-Cult 3307 CO₂ Incubator was engineered with contamination control as a primary design objective, combining Class 100 HEPA-filtered air quality, a 140°C in-place sterilization cycle, and precise environmental regulation within a compact 8.2 cubic foot (232-liter) chamber.
This refurbished unit from American Instrument Exchange has also been upgraded with a new dual-beam infrared (IR) CO₂ sensor, providing improved CO₂ measurement compared with the original thermal conductivity configuration. Infrared sensing delivers accurate atmospheric control with reduced sensitivity to chamber humidity, making the incubator an excellent choice for demanding mammalian cell culture applications requiring long-term environmental stability.
Key Features
Approximately 8.2 cu. ft. (232 L) chamber capacity
Upgraded dual-beam infrared (IR) CO₂ sensor
CO₂ control from 0% to 20%
Temperature range from ambient +5°C to 50°C
Class 100 in-chamber HEPA filtration
Automatic 140°C sterilization cycle
Controlled humidity to 95% RH
Stainless steel chamber interior
Heated inner glass door
Digital microprocessor controls
LED display
Up to 22 adjustable shelf positions
Audible and visual alarms
Remote alarm contacts
Interior accessory outlet
90-day warranty from American Instrument Exchange
Designed for Contamination-Sensitive Cell Culture
The Thermo Steri-Cult 3307 was developed for laboratories where maintaining sterile culture conditions is as important as controlling temperature and CO₂. Rather than relying solely on routine cleaning procedures, the incubator continuously filters recirculated chamber air through a high-efficiency HEPA filter while providing a programmable high-temperature sterilization cycle that simplifies decontamination between research projects.
The compact chamber accommodates tissue culture flasks, Petri dishes, multiwell plates, spinner flasks, and other commonly used cell culture vessels while providing enough capacity for multiple investigators or independent research projects. Up to 22 adjustable shelf positions allow laboratories to configure the chamber for different vessel heights and maximize usable incubation space.
The upgraded infrared CO₂ sensor further improves long-term environmental stability by providing dependable CO₂ measurement that is largely unaffected by changes in chamber humidity. Combined with controlled humidity and precise temperature regulation, the Steri-Cult 3307 creates a reliable environment for maintaining sensitive mammalian cell cultures over extended incubation periods.
Common Applications
The Thermo Steri-Cult 3307 is commonly used for:
Mammalian cell culture
Primary cell culture
Stem cell research
Cancer biology
Hybridoma production
Tissue engineering
Virology research
Protein expression
Monoclonal antibody development
Pharmaceutical research
Biotechnology research
Clinical laboratory research
Academic life science research
Long-term maintenance of valuable cell lines
Its combination of HEPA filtration, infrared CO₂ sensing, and automated sterilization makes it particularly valuable for laboratories where contamination prevention is a top priority.
Ideal Laboratory Environments
The Thermo Steri-Cult 3307 is well suited for biotechnology companies, pharmaceutical laboratories, university research facilities, hospital research centers, government laboratories, and contract research organizations performing routine mammalian cell culture.
Its compact footprint makes it an excellent solution for laboratories requiring advanced contamination control without dedicating space to a larger reach-in incubator. It is equally appropriate as a primary incubator for smaller laboratories or as a dedicated incubator for sensitive cell lines requiring additional isolation.
Temperature Recovery and Environmental Stability
The Thermo Steri-Cult 3307 maintains temperatures from 5°C above ambient to 50°C using a microprocessor-controlled heating system designed to deliver excellent temperature stability and chamber uniformity. Controlled relative humidity of up to 95% RH helps minimize evaporation of cell culture media, preserving consistent osmolarity during long incubation periods.
The upgraded dual-beam infrared CO₂ sensor accurately regulates CO₂ concentrations from 0% to 20%, while the heated inner glass door reduces condensation and allows researchers to inspect cultures without exposing the entire chamber to ambient laboratory conditions. Together, these systems provide rapid recovery following door openings while maintaining a stable environment for sensitive cell culture applications.
Advanced Contamination Control
One of the defining advantages of the Steri-Cult 3307 is its layered approach to contamination prevention. During normal operation, an in-chamber HEPA filtration system continuously removes airborne particulates to maintain Class 100 air quality, helping protect cultures from contaminants introduced during routine laboratory use.
When periodic decontamination is required, the incubator's 140°C in-place sterilization cycle sanitizes internal chamber surfaces without extensive manual disassembly. Access-code protection prevents accidental activation, while integrated safety monitoring helps ensure reliable operation throughout the sterilization process. Combined with the polished stainless steel interior and removable chamber components, these features simplify routine maintenance while supporting reproducible cell culture over the long term.
Sample Storage Recommendations
With approximately 8.2 cubic feet (232 liters) of usable chamber space, the Thermo Steri-Cult 3307 provides ample capacity for routine mammalian cell culture while maintaining a compact laboratory footprint. The chamber accommodates T-flasks, culture dishes, multiwell plates, spinner flasks, cell culture bags, roller bottles, and other commonly used tissue culture vessels, making it well suited for research laboratories managing multiple cell lines simultaneously.
The adjustable shelving system allows users to configure the interior for a variety of vessel sizes while preserving adequate airflow throughout the chamber. To maintain consistent temperature, humidity, and CO₂ conditions, culture vessels should be spaced evenly and should not obstruct air circulation. Proper organization also helps reduce unnecessary door openings, improving environmental stability during long-term incubation.
Many laboratories dedicate individual shelves or sections of the incubator to specific cell lines, investigators, or research projects. This organization minimizes handling, reduces cross-contamination risk, and streamlines daily laboratory workflows.
Energy Efficiency and Operating Costs
The Thermo Steri-Cult 3307 was engineered for continuous operation in research laboratories where dependable environmental control is required every day. Its insulated cabinet construction, digital microprocessor controls, and efficient heating system work together to maintain stable incubation conditions while minimizing unnecessary energy consumption.
The in-chamber Class 100 HEPA filtration system continuously cleans recirculated air without requiring excessive airflow, helping maintain a clean incubation environment while supporting efficient operation. The heated inner glass door reduces condensation and allows researchers to inspect cultures before opening the chamber, limiting temperature and CO₂ loss during routine observation.
The integrated 140°C sterilization cycle also helps reduce routine maintenance requirements by simplifying chamber decontamination. Rather than relying exclusively on manual cleaning procedures, laboratories can periodically perform automated sterilization between projects, improving laboratory efficiency while helping protect valuable cultures from contamination.
Key Specifications
Specification
Details
Manufacturer
Thermo
Model
Steri-Cult 3307
Incubator Type
Water-Jacketed CO₂ Incubator
Chamber Capacity
Approximately 232 L (8.2 cu. ft.)
Temperature Range
Ambient +5°C to 50°C
CO₂ Range
0% to 20%
CO₂ Sensor
Dual-Beam Infrared (IR)
Humidity Control
Up to 95% Relative Humidity
Air Quality
Class 100 HEPA Filtration
Sterilization
Automatic 140°C In-Place Sterilization Cycle
Chamber Material
Stainless Steel
Door Configuration
Heated Inner Glass Door with Insulated Outer Door
Controller
Digital Microprocessor
Display
LED Digital Display
Shelf Positions
Up to 22
Alarm System
Audible, Visual, and Remote Alarm Contacts
Interior Accessory Outlet
Yes
Electrical Requirements
120 V
Approximate Weight
Approximately 375 lb
Specifications are believed to be accurate but may vary depending on factory-installed options and the upgraded infrared CO₂ sensor installed on this unit. Please verify all specifications prior to purchase.
Frequently Asked Questions
What makes this Thermo Steri-Cult 3307 different from a standard Steri-Cult incubator?
This refurbished unit has been upgraded with a dual-beam infrared CO₂ sensor, providing more accurate CO₂ measurement than the original thermal conductivity sensor. Combined with Class 100 HEPA filtration and the integrated sterilization cycle, it offers enhanced environmental control for contamination-sensitive cell culture applications.
Why is an infrared CO₂ sensor preferred for many cell culture applications?
Infrared sensors provide highly accurate CO₂ measurement and are largely unaffected by humidity changes inside the chamber. This results in faster recovery following door openings and more stable atmospheric conditions for sensitive mammalian cell cultures.
What is the purpose of the Class 100 HEPA filtration system?
The HEPA filtration system continuously cleans recirculated chamber air, removing airborne particles that could contribute to contamination. Maintaining Class 100 air quality helps protect valuable cell cultures during routine laboratory operation.
What does the 140°C sterilization cycle accomplish?
The automatic sterilization cycle heats the chamber to approximately 140°C, reducing microbial contamination on interior surfaces without requiring extensive manual cleaning or chamber disassembly. Routine sterilization helps maintain a cleaner incubation environment between research projects.
What types of laboratories commonly use the Steri-Cult 3307?
The Steri-Cult 3307 is frequently found in biotechnology companies, pharmaceutical research laboratories, universities, hospital research centers, and government laboratories performing mammalian cell culture, stem cell research, cancer biology, virology, and tissue engineering.
Is the 3307 suitable for long-term maintenance of valuable cell lines?
Yes. The combination of stable temperature control, precise infrared CO₂ regulation, controlled humidity, HEPA-filtered chamber air, and integrated sterilization makes the incubator an excellent choice for maintaining valuable cell cultures over extended periods.
Can multiple users share the same incubator?
Yes. The chamber provides sufficient capacity for multiple investigators while the adjustable shelving system allows cultures to be organized by project or cell line. Proper organization and routine sterilization help maintain efficient workflows and reduce contamination risks in shared laboratory environments.
Why purchase a refurbished Thermo Steri-Cult 3307 from American Instrument Exchange?
Purchasing a refurbished Thermo Steri-Cult 3307 from American Instrument Exchange provides laboratories with an advanced CO₂ incubator featuring an upgraded infrared CO₂ sensor, Class 100 HEPA filtration, and automated sterilization at substantial savings compared with buying new equipment. Every incubator is inspected and tested prior to shipment and includes a 90-day warranty, providing confidence that it is ready to support demanding cell culture research.
Click Hereto see the Thermo 3307 on the American Instrument Exchange YouTube channel.
Testing Procedures for Used and Refurbished Laboratory Incubators Ensure Quality and Stability Before Use
Used and refurbished laboratory incubators from American Instrument Exchange are thoroughly tested to ensure optimal performance and temperature stability with laboratory safety standards. Each unit undergoes a detailed inspection and functionality assessment to meet the needs of research, clinical, and industrial labs.
Why Testing Used Laboratory Incubators Is Essential
Before a laboratory incubator is reintroduced into any work environment, it must be tested for:
• Temperature uniformity and accuracy
• Airflow and humidity control (if applicable)
• Door seal integrity and insulation effectiveness
• Electrical and safety system compliance
Comprehensive testing reduces the risk of culture loss, contamination, and equipment failure—especially critical in applications involving cell culture, microbiology, pharmaceutical research, and diagnostics.
Our multi-point testing protocol for used and refurbished incubators ensures reliability, safety, and performance across all models and brands.
Key Testing Procedures for Refurbished and Used Laboratory Incubators
1. Initial Visual Inspection
• Check for cosmetic damage, corrosion, and wear to exterior surfaces, door hinges, and gasket seals
• Inspect power cord, wiring, and display panel for any defects or signs of tampering
• Confirm that shelves, chamber components, and internal fixtures are present and undamaged
2. Functional Testing
• Power on the incubator and verify all display indicators and alarms function correctly
• Test door latch and seal mechanisms for proper closure and insulation
• Run basic temperature cycles to ensure the unit maintains stability over time
3. Temperature Accuracy and Uniformity Tests
• Test internal sensors and controllers using temperature probes
• Measure temperature consistency at multiple chamber locations over several hours
• Confirm that programmable temperature settings respond accurately
4. Airflow and Humidity Control (if applicable)
• Test internal fan operation and airflow distribution
• For humidified incubators, inspect and test humidity reservoirs, sensors, and control systems
• Check for condensation buildup and confirm environmental stability
5. Electrical Safety and Compliance Checks
• Ensure all electrical components are properly grounded
• Perform insulation resistance and leakage current tests
• Verify compliance with current electrical safety standards for laboratory equipment
6. Final Quality Assurance Checklist
• Perform full cycle testing at various temperature setpoints
• Document performance benchmarks and results
All refurbished incubators from American Instrument Exchange are backed by our commitment to quality and precision. With our strict testing procedures, you can rely on your incubator to deliver dependable results from day one.
Repair and Refurbishment Protocol for Used Laboratory Incubators Performance and Quality from American Instrument Exchange
At American Instrument Exchange, every used laboratory incubator we sell goes through a repair and refurbishment protocol. Whether it’s a standard, CO₂, or refrigerated incubator, our goal is to restore each unit to laboratory-level performance—ensuring temperature accuracy, environmental stability, and long-term reliability in your lab.
Why Choose a Refurbished Laboratory Incubator from American Instrument Exchange?
• Fully tested for performance and safety
• Backed by a warranty
• Cost-effective alternative to new equipment
• Environmentally sustainable through reuse and restoration
• Refurbished by expert technicians with decades of lab equipment experience
Step-by-Step Refurbishment Protocol
Every incubator at American Instrument Exchange follows this comprehensive, multi-stage process:
1. Initial Intake & Inspection
• Visual inspection for cosmetic wear, corrosion, dents, or broken components
• Mechanical assessment of door hinges, gaskets, seals, and chamber components
• Document any missing parts or issues requiring repair or replacement
2. Cleaning and Decontamination
• Thorough internal and external cleaning using non-abrasive solutions
• Decontamination/cleaning of surfaces to remove biological, chemical, or particulate residue
• Cleaning of removable parts (e.g., shelves, trays, etc.)
3. Mechanical and Electrical Repair
• Repair or replacement of worn mechanical components such as door latches, fans, hinges, and seals
• Evaluation and repair of control panels, switches, thermostats, and displays
• Inspection and testing of wiring, fuses, relays, and power supplies
4. Environmental Control System Assessment
• For CO₂ incubators: CO₂ sensors, regulators, and gas delivery systems are tested
• For refrigerated incubators: compressors, condensers, and cooling systems are evaluated for performance and leaks
• Fan motors and airflow systems are tested to ensure uniform environmental conditions
5. Temperature and Sensor Testing
• All temperature sensors and controllers are tested using thermometers/probes
• Uniformity and accuracy tested at multiple chamber locations
• For humidified or CO₂ incubators, humidity and CO₂ stability are verified
6. Safety System Testing
• Test of over-temperature protection systems and alarms
• Evaluation of door sensors, safety interlocks, and power failure recovery
7. Final Quality Control and Documentation
• Full performance test run over 24 hours
• Validation of setpoints for temperature, CO₂ (if applicable), and humidity (if applicable)
• Final inspection and cleaning before packaging
• Complete service report
Trust the Experts at American Instrument Exchange
With over 50 years in the lab equipment industry, American Instrument Exchange is a trusted source for high-quality refurbished laboratory incubators. Our proven refurbishment protocol ensures every unit is reliable, accurate, and ready to meet the demands of your lab from day one.
Warranty Information
All used equipment sold by American Instrument Exchange is refurbished/reconditioned, tested to meet specifications, cleaned, and comes with a 90-day warranty from the date of shipment unless otherwise specified. New products include a warranty from the manufacturer. Your warranty is only valid if you have paid your invoice. If your invoice is over 30 days old, warranty work will not be performed until your invoice is paid in full. American Instrument Exchange is not liable for any special or consequential damages that result from the use of, or the inability to use, the materials on this site or the performance of the products,
Exceptions include international sales and items that are agreed to be sold with a lesser warranty or items that are agreed to be sold “as is.”
Should there be visible shipping damage to packaging or concealed shipping damage to item(s) supplied, buyer must communicate such damage to the delivering entity or carrier upon receipt first and to American Instrument the same day item is accepted / signed for. Failure to do so voids warranty. Customer decision to involve / procure third party or OEM service other than American Instrument or AIE assigned vendors to resolve in-warranty instrument failure or defect without first placing a service call to American Instrument voids warranty.
Return & Cancellation Policy
American Instrument Exchange will authorize the return of and issue a full refund for any equipment that is not working as represented and could not be replaced or repaired to the customer’s satisfaction by AIE or a representative of AIE during the warranty period. Returns without prior authorization and an RMA number will not be accepted.
Customers are responsible for proper packaging of the equipment and all included accessories. American Instrument will pay for insurance and shipping costs. Non-inventory items bought specifically to fill customer orders may possibly be returned, but on a case by case basis.
Customers who cancel orders after refurbishing has begun or after delivery where there are no equipment performance issues may be subject to a Restocking/Cancellation Fee of 20% or more and responsible for shipping costs for both the delivery and return.
Ongoing and Extended Support Options
American Instrument Exchange is able to provide additional repair and support services outside the warranty terms for most products that sell.
For biosafety cabinets, clean benches, chemical fume hoods, and other clean air products our team at AIE Laboratory Services can provide certifications, repairs, decontaminations, and other services for customers throughout New England, and guidance and limited technical assistance for any customers in the US and Canada for new or refurbished biosafety cabinets purchased from American Instrument Exchange.
For all other products, the repair service team at American Instrument Exchange can provide both field and depot repair evaluations. We can easily schedule and coordinate the pick-up and transportation of your malfunctioning equipment. By offering hourly rates lower than most OEM service providers and eliminating travel time for technicians, we help reduce repair costs. Furthermore, our lab depot repair service minimizes or eliminates scheduling delays. Our experienced service technicians are qualified to restore your equipment to factory specifications.
Yes, all equipment from American Instrument Exchange comes with a warranty. Used/reconditioned/refurbished equipment is tested to make sure it operates to the manufacturer's specifications and comes with a 90-day warranty (unless otherwise noted). New equipment comes with a warranty from the manufacturer.
How do you test and recondition the equipment?
Testing requirements vary across all the equipment categories we sell. Temperature controlled units are tested with NIST traceable probes and data loggers. Gaskets, external housing, glass, handles, controls and control screens are all thoroughly inspected for integrity and functionality. We test all equipment using the manufacturer's parameters and specifications to confirm that the unit operates properly.
How do products ship from American Instrument Exchange?
We can ship products both nationally and internationally using a well-established network of transporters. Smaller items will ship via UPS. Larger equipment will be palletized and shipped either LTL or air-ride, depending on the specific equipment and/or delivery requirements.
Return and order cancellation policy
American Instrument Exchange will accept the return of any product that is not working as represented and could not be replaced or repaired to the customer's satisfaction during the warranty period. Customers who cancel orders after refurbishing has begun will be subject to a cancellation and restocking fee of between 20% and 40%.
Are discounts available for educational or non-profit institutions, or for bulk purchases?
Yes – call or email us today with a list of equipment you're looking for and we can potentially put together a quote that includes a discount.
How does your price matching policy work?
We strive to keep our pricing competitive and reasonable. If you find comparable items for sale at a lower price from other vendors please send us a link and or quote for the item and we'll do our best to match or beat the price.
Can I see the equipment in person before committing to a purchase?
Customers are welcome to make an appointment to view equipment in our warehouse. Given our workload we may not be able to test or clean the equipment prior to your arrival if you have not committed to an order.
1023 Western Avenue is a working warehouse, not a showroom, and customers should dress appropriately and wear closed toe shoes.
Can I ship products that I purchased elsewhere to American Instrument for repair?
Yes – please see our repair services page for more information.
