Thermo Scientific 1335 4' Biosafety Cabinet

Thermo Scientific 1335 4' Class II Type A2 Biosafety Cabinet

Refurbished Thermo Scientific 1335 Biosafety Cabinet for Sale

The Thermo Scientific 1335 is a 4-foot Class II Type A2 biosafety cabinet designed to provide personnel, product, and environmental protection for cell culture, microbiology, pharmaceutical research, biotechnology, clinical research, and other contamination-sensitive biological applications. Part of Thermo Scientific's proven 1300 Series A2 platform, the 1335 combines a space-efficient 4-foot footprint with an 8-inch working sash opening, one-piece 304 stainless steel interior, intelligent airflow management, and energy-efficient operation.

With approximately 47.2 inches of interior width, the Thermo 1335 provides ample room for common cell culture and biological research procedures while occupying substantially less laboratory space than a 5- or 6-foot biosafety cabinet. Researchers can organize culture vessels, pipettes, media bottles, sample racks, reagent containers, and other materials within the work area without dedicating unnecessary wall space to a larger cabinet.

The 8-inch operating sash opening is an important characteristic of the 1335. This lower operating aperture distinguishes it from closely related Thermo 1300 Series cabinets configured with a 10-inch opening and can be particularly useful for laboratories replacing an existing 8-inch-opening BSC or standardizing multiple workstations around the same operating configuration.

The 1335 also features a one-piece 304 stainless steel interior and stainless steel work surface. The smooth, durable chamber is well suited to frequent cleaning and disinfection, making the cabinet an attractive option for laboratories where contamination control and routine interior cleaning are important parts of daily operations.

At American Instrument Exchange, every Thermo Scientific 1335 biosafety cabinet is inspected and tested by our technicians prior to shipment. Unless otherwise specified, new HEPA filters are installed at our facility before delivery. This refurbished unit is backed by a 90-day warranty from American Instrument Exchange. Laboratories outfitting cell culture and biological research facilities may also browse additional refurbished biosafety cabinets, CO₂ incubators, centrifuges, laboratory refrigerators, freezers, and other laboratory equipment available through American Instrument Exchange.

Key Features

• Class II Type A2 biosafety cabinet

• 4-foot nominal cabinet width

• 8-inch working sash opening

• One-piece 304 stainless steel interior

• 304 stainless steel work surface

• Approximately 47.2-inch-wide interior workspace

• Space-efficient 51.2-inch exterior width

• HEPA-filtered vertical downflow and exhaust

• 99.995% HEPA filtration at MPPS

• Intelligent airflow management and monitoring

• Energy-efficient motor technology

• Reduced-flow operating capability

• 21-inch maximum sash opening for loading and cleaning

• 120V electrical configuration

• Designed for routine cell culture and biological research

• New HEPA filters included unless otherwise specified

• 90-day warranty from American Instrument Exchange

Overview

The Thermo Scientific 1335 combines the biological containment capabilities of the 1300 Series A2 platform with a practical 4-foot footprint, stainless steel work chamber, and 8-inch operating opening.

As a Class II Type A2 biosafety cabinet, the 1335 is designed to provide three forms of protection. Inward airflow through the front opening helps protect personnel from aerosols generated within the work chamber. HEPA-filtered vertical downflow helps protect samples and materials from airborne contamination, while exhaust air passes through HEPA filtration before leaving the cabinet.

The cabinet's 4-foot format makes it particularly useful in laboratories where space must be allocated among multiple pieces of equipment. At approximately 51.2 inches wide externally, the 1335 provides nearly four feet of usable interior width while preserving valuable laboratory wall and floor space for CO₂ incubators, centrifuges, refrigerators, microscopes, and other equipment.

The one-piece stainless steel chamber is another significant advantage. The 304 stainless steel side and rear walls, together with the stainless steel work surface, provide a durable interior designed to withstand routine cleaning and disinfection associated with cell culture and other contamination-sensitive workflows.

Why Choose the Thermo Scientific 1335?

The Thermo Scientific 1335 is particularly well suited for laboratories prioritizing space efficiency, stainless steel construction, and an 8-inch operating aperture.

For many routine cell culture procedures, a 4-foot cabinet provides an effective balance between usable workspace and laboratory footprint. The 1335 offers approximately 47 inches of interior width—enough room for common culture vessels, pipettes, media bottles, tube racks, aspiration equipment, and other materials—without consuming the additional wall space required by a 5- or 6-foot BSC.

The stainless steel chamber further distinguishes the 1335 from Thermo 1300 Series configurations equipped with alternative interior finishes. Laboratories that prefer stainless steel because of its durability and straightforward cleaning characteristics may therefore find the 1335 particularly attractive.

The 8-inch operating aperture can also be advantageous when replacing an existing cabinet or standardizing multiple BSCs throughout a facility. Researchers already accustomed to an 8-inch opening can maintain a familiar working configuration while gaining the proven airflow management and energy-efficient operation of the Thermo 1300 Series platform.

For biotechnology companies, pharmaceutical laboratories, universities, hospitals, startups, and other life-science organizations, a professionally refurbished Thermo 1335 provides a strong combination of containment performance, manageable footprint, durable construction, and acquisition value.

A Space-Efficient Stainless Steel BSC for Cell Culture

Laboratory floor space is valuable, particularly in cell culture rooms where biosafety cabinets compete for space with CO₂ incubators, centrifuges, microscopes, refrigerators, freezers, and other equipment.

The Thermo 1335's 4-foot configuration helps laboratories make efficient use of that space. Its approximately 51.2-inch exterior width allows the cabinet to fit into laboratory layouts where a larger BSC might restrict equipment placement or workflow, while its 47.2-inch interior width still provides substantial room for a single operator performing routine biological procedures.

This can also make the 1335 attractive when outfitting laboratories with multiple workstations. Depending on workflow requirements, installing several 4-foot BSCs can allow different researchers, cell lines, or projects to maintain dedicated work areas rather than relying on a smaller number of larger shared cabinets.

Combined with the one-piece stainless steel chamber, the 1335 provides a particularly practical configuration for cell culture facilities where cabinets are used and cleaned frequently.

Thermo Scientific 1335 vs. Thermo Scientific 1345

The Thermo Scientific 1335 and 1345 are closely related 4-foot Class II Type A2 biosafety cabinets, and distinguishing between them is particularly useful when purchasing refurbished equipment.

Both models use one-piece 304 stainless steel interior construction and the same basic 4-foot Thermo 1300 Series platform. The major difference is the operating aperture.

The Thermo 1335 has an 8-inch working sash opening, while the Thermo 1345 has a 10-inch working opening.

For laboratories replacing an existing 8-inch-opening cabinet or standardizing around that configuration, the 1335 can be the better fit. Laboratories that prefer greater vertical access to the chamber may instead consider the 10-inch-opening 1345.

This is a useful distinction when comparing refurbished Thermo cabinets because the two models otherwise share many characteristics. The correct choice may ultimately depend on operator preference, existing laboratory standards, application requirements, and current equipment availability.

Compatible Laboratory Equipment

The Thermo Scientific 1335 fits naturally into cell culture, biotechnology, pharmaceutical, microbiology, and clinical research workflows where samples routinely move between several pieces of laboratory equipment.

For cell culture applications, the 1335 is frequently paired with CO₂ incubators for maintaining cultures, refrigerated centrifuges for harvesting and processing samples, laboratory refrigerators for storing media and reagents, and ultra-low temperature freezers for long-term preservation of biological materials.

Inverted microscopes, laboratory autoclaves, water baths, cryogenic storage systems, and additional biosafety cabinets can further support complete biological research workflows.

American Instrument Exchange maintains a broad inventory of professionally refurbished laboratory equipment, allowing laboratories to source multiple compatible instruments through a single supplier while simplifying procurement and reducing equipment lead times.

Common Applications

• Mammalian cell and tissue culture

• Stem cell research

• Molecular biology

• Microbiology

• Virology

• Pharmaceutical research and development

• Biotechnology research

• Vaccine research

• Gene and cell therapy research

• Clinical and diagnostic laboratory procedures

• Sample preparation

• University and government life-science research

Ideal Laboratory Environments

The Thermo Scientific 1335 is an excellent choice for biotechnology companies, pharmaceutical research laboratories, university research centers, hospital laboratories, contract research organizations, clinical facilities, startup laboratories, and other life-science organizations requiring Class II Type A2 containment.

Its combination of a 4-foot footprint, 8-inch operating opening, one-piece stainless steel chamber, and proven Thermo 1300 Series airflow platform makes the 1335 particularly useful for laboratories that want a durable, space-efficient BSC for routine biological research.

Key Specifications

Specifications compiled from Thermo Scientific manufacturer literature and American Instrument Exchange product information.

Why Buy a Refurbished Thermo Scientific 1335 Biosafety Cabinet?

The Thermo Scientific 1335 offers a practical combination of Class II Type A2 containment, compact 4-foot dimensions, stainless steel construction, and the proven Thermo 1300 Series platform. For laboratories performing routine cell culture, microbiology, pharmaceutical research, and other biological procedures, a professionally refurbished 1335 can provide the capabilities of a modern research BSC while substantially reducing acquisition cost compared with purchasing new.

Its 4-foot configuration is particularly useful in laboratories where space must be shared among biosafety cabinets, CO₂ incubators, centrifuges, microscopes, refrigerators, freezers, and other equipment. The approximately 47.2-inch-wide interior provides substantial workspace for a single operator while the approximately 51.2-inch exterior width helps conserve valuable laboratory floor and wall space.

The 1335 also combines its compact footprint with an 8-inch operating opening and one-piece 304 stainless steel interior. This configuration can be particularly attractive for laboratories replacing an existing 8-inch-opening cabinet, standardizing multiple workstations around the same sash height, or simply preferring the durability and cleanability of a stainless steel chamber.

For laboratories comparing several used Thermo 1300 Series cabinets, understanding these model-specific differences is important. The 1335 is not simply another generic 4-foot Thermo BSC—it represents a specific combination of 4-foot width, 8-inch operating aperture, and stainless steel interior construction.

Refurbishment & Testing Process

Every Thermo Scientific 1335 offered by American Instrument Exchange undergoes a detailed inspection, cleaning, refurbishment, and testing process prior to shipment. Our technicians evaluate the cabinet's mechanical, electrical, control, airflow-related, and filtration systems and identify components requiring repair or replacement.

Unless otherwise specified, new HEPA filters are installed at our facility before shipment. The blower system, airflow monitoring system, alarms, lighting, electrical components, sash operation, and other cabinet functions are inspected and tested for proper operation.

The stainless steel work chamber and removable work surface are professionally cleaned and inspected. Worn or damaged functional components are repaired or replaced whenever practical as part of the refurbishment process.

Our objective is not simply to determine whether a used biosafety cabinet powers on. Each refurbished Thermo 1335 is prepared for installation and subsequent field certification so that it can return to productive laboratory service following delivery.

Certification Readiness

The Thermo Scientific 1335 is a Class II Type A2 biosafety cabinet designed to provide personnel, product, and environmental protection when properly installed, operated, and certified.

Proper field certification remains essential after installation because transportation, placement, room airflow, and other site-specific conditions can affect cabinet performance. Certification evaluates critical operating characteristics including inflow and downflow velocities, HEPA filter integrity, airflow patterns, alarms, and other parameters applicable to Class II Type A2 cabinet operation.

Biosafety cabinets should also be recertified at appropriate intervals and following events that may affect containment performance, including relocation, HEPA filter replacement, or significant internal service.

For laboratories throughout Greater Boston and New England, AIE Lab Services provides biosafety cabinet certification, preventative maintenance, troubleshooting, repairs, HEPA filter replacement, decontamination, relocation, and recurring certification services.

This gives laboratories purchasing a refurbished Thermo Scientific 1335 from American Instrument Exchange access to an affiliated service organization that can continue supporting the cabinet after installation.

Shipping & Installation

The Thermo Scientific 1335 is professionally prepared and packaged for commercial freight shipment from American Instrument Exchange. Its 4-foot footprint can simplify placement compared with larger biosafety cabinets, but customers should still evaluate the complete delivery route before shipment.

Doorway widths, hallways, elevators, loading areas, ceiling clearance, electrical service, and the final installation location should all be verified. The cabinet should also be positioned with consideration for doors, HVAC registers, pedestrian traffic, and other sources of disruptive air movement that could affect containment performance.

The approximately 51.2-inch exterior width makes the 1335 particularly attractive for equipment-dense laboratories and smaller cell culture rooms. Adequate clearance should still be provided for installation, service access, and proper operation.

American Instrument Exchange can assist with freight coordination, inside delivery, equipment placement, and installation planning when required.

After the 1335 reaches its final operating location, the cabinet should be field certified before being placed into biological service.

Related Laboratory Equipment

The Thermo Scientific 1335 is frequently installed as part of a complete cell culture, biotechnology, pharmaceutical, microbiology, or clinical research workflow.

For cell culture applications, the 1335 can be paired with CO₂ incubators for maintaining cultures, refrigerated centrifuges for sample processing and harvesting, laboratory refrigerators for media and reagent storage, and ultra-low temperature freezers for long-term preservation of biological samples.

Inverted microscopes, laboratory autoclaves, water baths, cryogenic storage systems, and additional biosafety cabinets can further support complete research workflows.

American Instrument Exchange maintains a broad inventory of professionally refurbished laboratory equipment, allowing laboratories to source multiple pieces of compatible equipment through a single supplier while simplifying procurement and reducing equipment lead times.

Thermo Scientific 1335 Frequently Asked Questions

What type of biosafety cabinet is the Thermo Scientific 1335?

The Thermo Scientific 1335 is a 4-foot Class II Type A2 biosafety cabinet from the Thermo 1300 Series. It is designed to provide personnel, product, and environmental protection for appropriate biological laboratory procedures.

The 1335 is specifically configured with an 8-inch working sash opening and one-piece 304 stainless steel interior.

Is the Thermo Scientific 1335 a 4-foot biosafety cabinet?

Yes. The Thermo 1335 is a 4-foot nominal-width BSC. It measures approximately 51.2 inches wide externally and provides approximately 47.2 inches of interior working width.

This makes it a practical option for laboratories that need a full-size biological work area while conserving floor and wall space compared with 5- or 6-foot cabinets.

Does the Thermo 1335 have an 8-inch or 10-inch sash opening?

The Thermo Scientific 1335 has an 8-inch working sash opening.

This is an important distinction when comparing Thermo 1300 Series cabinets because closely related models are available with different operating apertures. Laboratories replacing an existing 1335 or standardizing around an 8-inch sash height should take this specification into account when comparing refurbished cabinets.

What is the difference between the Thermo 1335 and Thermo 1345?

Both are 4-foot Class II Type A2 cabinets with one-piece stainless steel interiors, but their working sash openings differ.

The Thermo 1335 uses an 8-inch working opening, while the Thermo 1345 uses a 10-inch working opening.

The 1335 may therefore appeal to laboratories that prefer an 8-inch operating aperture or are replacing an existing cabinet with that configuration. The 1345 provides additional vertical access to the work area for laboratories that prefer a 10-inch opening.

Why choose an 8-inch-opening biosafety cabinet?

An 8-inch operating aperture can provide a comfortable working configuration for many routine cell culture, microbiology, and biological research procedures while maintaining a relatively compact front opening.

It can also be important when laboratories are replacing existing equipment. Facilities with multiple 8-inch-opening cabinets may prefer to maintain consistent operating configurations across different workstations.

The appropriate sash height ultimately depends on the cabinet design, application, operator preference, and laboratory requirements.

Does the Thermo 1335 have a stainless steel interior?

Yes. The Thermo Scientific 1335 features a one-piece 304 stainless steel interior and stainless steel work surface.

Stainless steel provides a durable, smooth chamber well suited to frequent cleaning and disinfection. This can be particularly attractive in cell culture laboratories and other environments where the BSC interior is cleaned routinely.

Is the Thermo 1335 suitable for cell culture?

Yes. The Thermo 1335 is well suited to many routine mammalian cell culture and tissue culture procedures requiring Class II Type A2 containment.

Its approximately 47-inch-wide work area provides room for culture vessels, pipettes, media bottles, tube racks, aspiration equipment, and other materials commonly used during cell culture procedures.

As with any BSC, operators should organize the work area carefully and avoid blocking the front and rear airflow grilles.

Should I choose a 4-foot or 6-foot biosafety cabinet?

A 4-foot cabinet such as the Thermo 1335 provides ample workspace for many procedures while conserving laboratory floor space. It can be particularly useful for individual operators, smaller cell culture rooms, startup laboratories, and facilities that want to install several dedicated workstations.

A 6-foot cabinet may be preferable when procedures require larger equipment, numerous culture vessels, unusually high sample volumes, or more extensive working space.

Laboratories should consider both their current workflow and future equipment needs when choosing cabinet width.

What do the airflow monitoring systems on the Thermo 1335 do?

The Thermo 1300 Series incorporates airflow management and monitoring designed to help maintain appropriate cabinet operating conditions as airflow resistance changes over time.

The cabinet provides operating information and alarms to alert users when conditions such as airflow or sash position move outside acceptable parameters.

These monitoring systems are useful during routine operation, but they do not replace periodic field certification of the biosafety cabinet.

What does a CAL message or airflow alarm on a Thermo 1335 mean?

A persistent CAL message or airflow alarm indicates that the cabinet's airflow or monitoring system requires evaluation. Possible causes can include calibration status, HEPA filter loading, blower performance, sash position, or another condition affecting airflow.

A cabinet displaying a persistent warning should be evaluated rather than assumed to be operating correctly.

For laboratories throughout New England, AIE Laboratory Services can troubleshoot Thermo 1300 Series biosafety cabinets, evaluate airflow performance, perform repairs or HEPA filter replacement when required, and recertify the cabinet following service.

Are new HEPA filters installed in this refurbished Thermo 1335?

Unless otherwise specified, American Instrument Exchange installs new HEPA filters prior to shipment.

The cabinet is also inspected, cleaned, refurbished, and functionally tested before leaving our facility. Once installed at the customer's laboratory, the BSC should be field certified before being placed into biological service.

Does the Thermo 1335 need to be certified after delivery?

Yes. A biosafety cabinet should be field certified after installation and before being placed into biological service.

Transportation and placement can affect HEPA filter integrity and cabinet operating conditions, while the surrounding laboratory environment can influence airflow performance.

Periodic recertification should also be incorporated into the laboratory's BSC maintenance program and performed following relocation, HEPA filter replacement, or service that could affect containment.

Can I see the actual Thermo 1335 before purchasing it?

Yes. American Instrument Exchange sells individual refurbished laboratory instruments, and customers can contact us with questions about the specific cabinet being offered.

Additional photographs or information may be available upon request. This can be useful when verifying cosmetic condition, stand configuration, utility fixtures, electrical configuration, or other details before purchasing refurbished equipment.

Can American Instrument Exchange arrange inside or white-glove delivery?

Depending on the destination and project requirements, American Instrument Exchange can assist with freight, inside delivery, and equipment-placement planning.

Although the 4-foot Thermo 1335 is easier to accommodate than many larger BSCs, it remains a large precision laboratory instrument. Customers requiring liftgate service, inside delivery, placement, or other special handling should discuss these requirements when requesting a quote.

I already own a Thermo 1335. Is it worth repairing?

In many cases, yes. The Thermo 1300 Series is a widely installed biosafety cabinet platform, and problems involving airflow calibration, blowers, controls, alarms, sash components, lighting, or HEPA filters do not necessarily mean the entire cabinet needs to be replaced.

The decision depends on the cabinet's overall condition, the component requiring repair, parts availability, repair cost, and whether the cabinet can continue to meet certification requirements.

For laboratories throughout New England, AIE Laboratory Services can evaluate an existing Thermo 1335 and help determine whether repair or replacement is the more practical option.

I purchased my Thermo 1335 somewhere else. Can AIE Laboratory Services still service it?

Yes. The cabinet does not need to have been purchased from American Instrument Exchange for AIE Laboratory Services to certify, maintain, troubleshoot, or repair it.

This is particularly useful for laboratories operating several Thermo 1300 Series cabinets acquired at different times or from multiple equipment suppliers.

Can American Instrument Exchange help me replace an older Thermo 1335?

Yes. If an existing Thermo 1335 has reached the point where replacement is more practical than repair, American Instrument Exchange can help identify available refurbished cabinets with comparable dimensions and operating characteristics.

An exact 1335 may not always be available, but other 4-foot Thermo 1300 Series cabinets may provide similar installation dimensions. Particular attention should be paid to the 8-inch versus 10-inch working aperture and stainless steel versus alternative interior construction when selecting a replacement.

Do you sell replacement HEPA filters or parts for the Thermo 1335?

American Instrument Exchange generally retains available used components to support the refurbishment and repair of additional biosafety cabinets rather than routinely selling used parts individually. Depending on the component and current availability, however, we may be able to assist in identifying or sourcing an appropriate replacement.

Replacement HEPA filters can be sourced through manufacturers and qualified filter suppliers. Because HEPA filter replacement can involve exposure to the contaminated side of the filtration system, appropriate biosafety and decontamination procedures should be followed.

For customers in New England, AIE Laboratory Services can provide decontamination, HEPA filter replacement, testing, and subsequent certification.

Can American Instrument Exchange supply multiple matching 4-foot Thermo biosafety cabinets?

Depending on current inventory, yes. American Instrument Exchange frequently works with laboratories that need multiple biosafety cabinets for expansions, new research spaces, or replacement projects.

When several identical Thermo 1335 cabinets are not available, we may be able to identify closely related 4-foot Thermo 1300 Series models that provide compatible dimensions and performance characteristics.

Customers requiring several cabinets should contact American Instrument Exchange to discuss quantities, preferred configurations, and project timelines.

Why purchase a refurbished Thermo Scientific 1335 from American Instrument Exchange?

American Instrument Exchange specializes in professionally refurbished laboratory equipment rather than simply reselling used instruments. Each Thermo Scientific 1335 is inspected, cleaned, tested, and prepared for continued laboratory service by experienced technicians.

Unless otherwise specified, new HEPA filters are installed prior to shipment, and the unit is backed by a 90-day warranty from American Instrument Exchange. Laboratories throughout New England can also access ongoing biosafety cabinet certification, maintenance, troubleshooting, and repair services through AIE Laboratory Services.

For laboratories seeking a refurbished 4-foot Class II Type A2 biosafety cabinet with an 8-inch operating opening and stainless steel interior, the Thermo Scientific 1335 provides a particularly practical combination of space efficiency, durable construction, proven containment technology, and long-term value.

CLICK HERE to see the Thermo 1300 Series 4' A2 biosafety cabinet on our YouTube channel